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The map label for this gene is tyrS

Identifier: 16273500

GI number: 16273500

Start: 1675940

End: 1677145

Strand: Reverse

Name: tyrS

Synonym: HI1610

Alternate gene names: 16273500

Gene position: 1677145-1675940 (Counterclockwise)

Preceding gene: 16273501

Following gene: 30995460

Centisome position: 91.64

GC content: 39.83

Gene sequence:

>1206_bases
ATGACTGACATTAATACCGTTCTCGCGGAACTAAAGCGTGGTACTGATGAAATCCTTTCAGAAGCGGATTTAATCGAAAA
ACTGAAAGAAAATCGCCCATTAAAAGTAAAATTAGGCGCAGATCCAACCGCACCAGATATTCATTTAGGACATACTGTTG
TATTAAACAAATTACGTCAATTTCAACAACTCGGTCATGAAGTCTATTTCTTAATTGGCGATTTTACTGGAATGGTGGGC
GATCCATCGGGCAAAAATGCGACTCGTCCTCCACTTAGCCGTGAAGATGTATTGCGCAATGCGGAAACCTATAAAGAGCA
AATTTATAAAATTCTCGATCCACAAAAAACCAAAATCGTCTTCAACTCTGAATGGTTAAGCAAACTTGGTACAGRAGGTA
TGATTCGCCTTGCAAGCAACTATACGGTTGCGCGTATGCTTGAACGCGACGACTTCAAAAAACGCTTTGGAAACAACCAA
CCCATTGCCATCCATGAATTTATTTATCCATTACTACAAGGCTACGATTCCGTTGCATTAGACGCAGATGTGGAACTAGG
CGGTACAGACCAAAAATTCAACTTACTTGTTGGTCGTGAATTACAAAAATCAGCAGGCAAAAAACCACAGGTTGCGATTA
CACTCCCATTACTCGTTGGCTTAGATGGCGAGAAAAAAATGTCTAAATCACTTGGTAACTATATTGGCGTAACGGAAGCA
CCAAGCGATATGTTCGGTAAAGTGATGTCAATTTCTGATGAACTCATGTGGGATTGGTATAACCTTCTTTCATTCCGTCC
ATTAAACGAAATTGCACAATTAAAATCGGAAGTAGAAAACGGCAAAAATCCTCGTGATGTCAAAATCTTACTAGCAAAAG
AATTAATTGCACGTTTCCACAATGAAGAGGCAGCAAACGCTGCAGAACAAGAATTTATTAACCGTTTCCAAAAAGGCGCA
ATGCCTGATGAAATGCCTGAATTTACGTTCTCTGGCGAAATGGGTTTAGCCACCTTATTAAAAGAAGCAGGTCTTGTTCC
TTCCACTTCTGAAGCGATTCGCTCTGCTCAACAAGGCGGTGTAAAAATCAATGGTGAAAAAGTCGATAACGTAAAAGACA
ATGCACCGAAAGGTACAAATGTTTACCAAGTGGGTAAACGAAAATTTGCGCGTGTACGGTTGAATAAAGTAGATACAGTT
AAATAA

Upstream 100 bases:

>100_bases
ATTCCCGCCTATTTCCCCACAGATAGGCGTTTTTTTTTATTCACAAATTGCTAAGAATCCCTATAATAAAACCTTTTAAA
ACATAATTTAGGAAATAATA

Downstream 100 bases:

>100_bases
AGTAAATAAAAAAATACCGCACTTTGGCAATAAAAGTGCGGTATTTTTTATTGAAATTTTAATTTAGTTAAACATCGCAG
AAATAGATTCTTCGTTGCTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVG
DPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTXGMIRLASNYTVARMLERDDFKKRFGNNQ
PIAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
PSDMFGKVMSISDELMWDWYNLLSFRPLNEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGA
MPDEMPEFTFSGEMGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVRLNKVDTV
K

Sequences:

>Translated_401_residues
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVG
DPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTXGMIRLASNYTVARMLERDDFKKRFGNNQ
PIAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
PSDMFGKVMSISDELMWDWYNLLSFRPLNEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGA
MPDEMPEFTFSGEMGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVRLNKVDTV
K
>Mature_400_residues
TDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVYFLIGDFTGMVGD
PSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIVFNSEWLSKLGTXGMIRLASNYTVARMLERDDFKKRFGNNQP
IAIHEFIYPLLQGYDSVALDADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEAP
SDMFGKVMSISDELMWDWYNLLSFRPLNEIAQLKSEVENGKNPRDVKILLAKELIARFHNEEAANAAEQEFINRFQKGAM
PDEMPEFTFSGEMGLATLLKEAGLVPSTSEAIRSAQQGGVKINGEKVDNVKDNAPKGTNVYQVGKRKFARVRLNKVDTVK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=412, Percent_Identity=25.2427184466019, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787925, Length=376, Percent_Identity=28.4574468085106, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=223, Percent_Identity=31.8385650224215, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=419, Percent_Identity=25.5369928400955, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321624, Length=259, Percent_Identity=29.3436293436293, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=27.0471464019851, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_HAEIN (P43836)

Other databases:

- EMBL:   L42023
- PIR:   D64132
- RefSeq:   NP_439752.1
- GeneID:   950464
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1610
- NMPDR:   fig|71421.1.peg.1526
- TIGR:   HI_1610
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   HINF71421:HI_1610-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44722; Mature: 44591

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQ
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQQLGHEVYFLIGDFTGMVGDPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIV
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
FNSEWLSKLGTXGMIRLASNYTVARMLERDDFKKRFGNNQPIAIHEFIYPLLQGYDSVAL
ECHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
DADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
CCCEEECCCCCHHHHHHCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCC
PSDMFGKVMSISDELMWDWYNLLSFRPLNEIAQLKSEVENGKNPRDVKILLAKELIARFH
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NEEAANAAEQEFINRFQKGAMPDEMPEFTFSGEMGLATLLKEAGLVPSTSEAIRSAQQGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
VKINGEKVDNVKDNAPKGTNVYQVGKRKFARVRLNKVDTVK
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDINTVLAELKRGTDEILSEADLIEKLKENRPLKVKLGADPTAPDIHLGHTVVLNKLRQ
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQQLGHEVYFLIGDFTGMVGDPSGKNATRPPLSREDVLRNAETYKEQIYKILDPQKTKIV
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
FNSEWLSKLGTXGMIRLASNYTVARMLERDDFKKRFGNNQPIAIHEFIYPLLQGYDSVAL
ECHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
DADVELGGTDQKFNLLVGRELQKSAGKKPQVAITLPLLVGLDGEKKMSKSLGNYIGVTEA
CCCEEECCCCCHHHHHHCHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCCCC
PSDMFGKVMSISDELMWDWYNLLSFRPLNEIAQLKSEVENGKNPRDVKILLAKELIARFH
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NEEAANAAEQEFINRFQKGAMPDEMPEFTFSGEMGLATLLKEAGLVPSTSEAIRSAQQGG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
VKINGEKVDNVKDNAPKGTNVYQVGKRKFARVRLNKVDTVK
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800