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The map label for this gene is pitB [C]

Identifier: 16273494

GI number: 16273494

Start: 1670086

End: 1671348

Strand: Direct

Name: pitB [C]

Synonym: HI1604

Alternate gene names: 16273494

Gene position: 1670086-1671348 (Clockwise)

Preceding gene: 16273493

Following gene: 16273495

Centisome position: 91.25

GC content: 40.14

Gene sequence:

>1263_bases
ATGGAAATTATTAGTCAATATGGTTCTTGGTTGGTGTGGATCACAGCAGTCTTTGGCTTTTTTATGGCGTTCGGAATTGG
AGCGAATGATGTCTCAAATTCAATGGGAACATCAGTAGGATCTGGTACCATAACCGCCAAACAAGCGATTATTATCGCAT
TAATTTTTGAATCTGCTGGTGCCTATCTAGCAGGTGGTGAAGTAACACAAACCATTAAAAGTGGTGTAATCGAACCAATA
CAATTTGTCGATACGCCAGATATTTTAGCTCTAGGTATGCTTTCAACCTTATTTGCATCGGGTGCTTGGTTATTTATTGC
AACCAAAATGGGTTGGCCAGTTTCTGGTACACACACTATTATTGGTGCAATTATTGGATTTGCCTGTATTACGATTGGCC
CTAGTTCTGTAGATTGGTCAAAGATTGGCAGCATCGTTGGTAGCTGGTTTGTCACGCCTGTCATTGCGGGTATTTTGGCT
TATGCAATCTTTGCAAGTACGCAAAAACTTATTTTTGATACCGAACAACCACTAAAAAATGCACAAAAATATGGCCCTTA
TTATATGGGGATAACCGTGTTTGTACTTTGTATCGTGACGATGAAAAAAGGCTTAAAACACGTAGGCTTAAATCTTTCAA
ACAGTGAAACTTTAATCATTTCACTTGCCATCAGTCTTATCGGAATGTTTTTCTTCCATTTCTATTTTAAAAGCAAAATC
TTCACTCAATCAGCAAACAAAGGCACTTTTGGTGCAGTAGAAAAAGTCTTCAGTATTTTAATGTTATTAACCGCTTGTGC
GATGGCATTTGCGCACGGTTCTAATGACGTAGCCAATGCAATTGGCCCTCTTTCTGCAGTGGTCTCTATTGTAAATGAAG
GCGGTAAAATTGTTTCAGGTGGAGCCTTAACTTGGTGGATTTTACCCTTAGGTGCATTAGGTATTGCTGTGGGCTTAATT
ACTATGGGACAAAAAGTGATGGCGACTGTTGGATCTGGGATCACCGATTTAACGCCAAGCCGTGGCTTTGCCGCTCAATT
TGCTACTGCGATGACCGTTGTCGTGGCATCAGGCACAGGCTTACCCATCTCAACAACACAAACACTTGTTGGAGCTATTT
TAGGTATCGGCTTTGCACGTGGTATCGCAGCACTGAATTTAACGGTTATCCGAAACATCATTAGTTCTTGGATTGTCACA
TTACCAGCAGGTGCATTTTTCGCCATTATTATTTTCTATGTGCTAAGAACAATCTTTAATTAA

Upstream 100 bases:

>100_bases
TGAATGGGTTGGCGTTTTGGCTGACCAAGCACAACGTGTCGGTTCGCGCATTGAATTAATGCTCGCGCGTTCATAATCAA
CAAAACATAGGAAATAAACT

Downstream 100 bases:

>100_bases
TTGATCTCAATAAAAAGTGCGGTTAATTTTAACCGCACTTTTGTTGTGATGTTTAAGGGGAAAAAATGAAAAAGATCTAT
AAAGCCTTAATCTCATCTTT

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIEPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Sequences:

>Translated_420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIEPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN
>Mature_420_residues
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIEPI
QFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTIIGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Specific function: Potential transporter for phosphate

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family

Homologues:

Organism=Homo sapiens, GI5803173, Length=250, Percent_Identity=33.6, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI31543630, Length=291, Percent_Identity=34.020618556701, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25146401, Length=463, Percent_Identity=32.829373650108, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71981576, Length=454, Percent_Identity=32.15859030837, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17557328, Length=494, Percent_Identity=26.5182186234818, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17539280, Length=252, Percent_Identity=37.6984126984127, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566716, Length=235, Percent_Identity=32.3404255319149, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=34.9397590361446, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319773, Length=242, Percent_Identity=34.7107438016529, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356511, Length=197, Percent_Identity=41.1167512690355, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1604_HAEIN (P45268)

Other databases:

- EMBL:   L42023
- PIR:   A64132
- RefSeq:   NP_439746.1
- GeneID:   950410
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1604
- NMPDR:   fig|71421.1.peg.1520
- TIGR:   HI_1604
- HOGENOM:   HBG681698
- OMA:   RNGIIDS
- ProtClustDB:   CLSK2517317
- BioCyc:   HINF71421:HI_1604-MONOMER
- InterPro:   IPR001204
- PANTHER:   PTHR11101

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 44026; Mature: 44026

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb07e33c)-; HASH(0xe65cc20)-; HASH(0xec4b530)-; HASH(0xeafcc1c)-; HASH(0xe578444)-; HASH(0xddeab0c)-; HASH(0xb2cda60)-; HASH(0xe2f639c)-; HASH(0xe4658e4)-; HASH(0xe44317c)-; HASH(0xeb07b14)-; HASH(0xebfe064)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIEPIQFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTI
CEEECCHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
IGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEIISQYGSWLVWITAVFGFFMAFGIGANDVSNSMGTSVGSGTITAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIEPIQFVDTPDILALGMLSTLFASGAWLFIATKMGWPVSGTHTI
CEEECCHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
IGAIIGFACITIGPSSVDWSKIGSIVGSWFVTPVIAGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800