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The map label for this gene is sspA

Identifier: 16273348

GI number: 16273348

Start: 1529727

End: 1530365

Strand: Reverse

Name: sspA

Synonym: HI1441

Alternate gene names: 16273348

Gene position: 1530365-1529727 (Counterclockwise)

Preceding gene: 16273349

Following gene: 16273347

Centisome position: 83.62

GC content: 34.43

Gene sequence:

>639_bases
ATGTCCAGCGCATCAAGTAAACGTTCAGTAATGACCCTTTTTTCAAATAAAGATGATATTTATTGCCATCAGGTAAAAAT
TGTTTTGGCAGAAAAAGGTGTGCTTTATGAAAACGCAGAAGTTGATTTGCAAGCTCTACCAGAAGATTTAATGGAATTAA
ATCCATACGGCACTGTGCCTACGTTGGTTGATCGTGATCTCGTATTATTTAATTCACGCATCATAATGGAATATCTTGAT
GAACGTTTTCCACATCCTCCATTAATGCAAGTGTATCCTGTTTCTCGTGCTAAAGATCGACTTTTAATGTTACGTATTGA
ACAAGATTGGTATCCAACCCTTGCAAAAGCAGAAAATGGCACAGAAAAAGAAAAAACTTCAGCATTAAAACAGTTAAAAG
AAGAACTATTAGGGATTGCACCAATTTTTCAACAGATGCCTTATTTTATGAATGAAGAATTTGGTTTGGTTGATTGCTAT
GTTGCACCATTATTATGGAAATTAAAACATTTAGGTGTGGAATTTACAGGCACAGGAAGTAAAGCGATTAAAGCTTATAT
GGAACGTGTGTTTACACGTGATTCTTTTTTGCAATCTGTAGGTGAAGCTGCACCAAAAAATTTAATGGATGATAAATAA

Upstream 100 bases:

>100_bases
TAAAACAATAAAAAAGATTATATTGGCAAATAGGTAATGCTTTATGGTAAAATGTTGCCCCTTAAAAACCTAGAATATTT
TTGGATCTTCGGAGGAATAA

Downstream 100 bases:

>100_bases
TGGAATATAAATCTTCTCCTAAACGCCCTTATTTATTACGCGCCTATTATGATTGGCTAGTAGATAATAGTTTTACGCCT
TATTTAGTTGTTGATGCGAC

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSSASSKRSVMTLFSNKDDIYCHQVKIVLAEKGVLYENAEVDLQALPEDLMELNPYGTVPTLVDRDLVLFNSRIIMEYLD
ERFPHPPLMQVYPVSRAKDRLLMLRIEQDWYPTLAKAENGTEKEKTSALKQLKEELLGIAPIFQQMPYFMNEEFGLVDCY
VAPLLWKLKHLGVEFTGTGSKAIKAYMERVFTRDSFLQSVGEAAPKNLMDDK

Sequences:

>Translated_212_residues
MSSASSKRSVMTLFSNKDDIYCHQVKIVLAEKGVLYENAEVDLQALPEDLMELNPYGTVPTLVDRDLVLFNSRIIMEYLD
ERFPHPPLMQVYPVSRAKDRLLMLRIEQDWYPTLAKAENGTEKEKTSALKQLKEELLGIAPIFQQMPYFMNEEFGLVDCY
VAPLLWKLKHLGVEFTGTGSKAIKAYMERVFTRDSFLQSVGEAAPKNLMDDK
>Mature_211_residues
SSASSKRSVMTLFSNKDDIYCHQVKIVLAEKGVLYENAEVDLQALPEDLMELNPYGTVPTLVDRDLVLFNSRIIMEYLDE
RFPHPPLMQVYPVSRAKDRLLMLRIEQDWYPTLAKAENGTEKEKTSALKQLKEELLGIAPIFQQMPYFMNEEFGLVDCYV
APLLWKLKHLGVEFTGTGSKAIKAYMERVFTRDSFLQSVGEAAPKNLMDDK

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain

Homologues:

Organism=Escherichia coli, GI1789624, Length=203, Percent_Identity=62.5615763546798, Blast_Score=269, Evalue=1e-73,
Organism=Drosophila melanogaster, GI21355779, Length=165, Percent_Identity=30.9090909090909, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SSPA_HAEIN (P45207)

Other databases:

- EMBL:   L42023
- PIR:   E64123
- RefSeq:   NP_439593.1
- PDB:   3LYK
- PDBsum:   3LYK
- ProteinModelPortal:   P45207
- SMR:   P45207
- GeneID:   950832
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1441
- NMPDR:   fig|71421.1.peg.1375
- TIGR:   HI_1441
- HOGENOM:   HBG289890
- OMA:   MERQFAR
- ProtClustDB:   CLSK870144
- BioCyc:   HINF71421:HI_1441-MONOMER
- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:1.20.1050.10
- Gene3D:   G3DSA:3.40.30.10

Pfam domain/function: PF00043 GST_C; PF02798 GST_N; SSF47616 GST_C_like; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 24290; Mature: 24159

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50405 GST_CTER; PS50404 GST_NTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSASSKRSVMTLFSNKDDIYCHQVKIVLAEKGVLYENAEVDLQALPEDLMELNPYGTVP
CCCCCHHHHHHHEECCCCCEEEEEEEEEEECCCEEECCCCCCHHHCCHHHHHCCCCCCCC
TLVDRDLVLFNSRIIMEYLDERFPHPPLMQVYPVSRAKDRLLMLRIEQDWYPTLAKAENG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHCCCC
TEKEKTSALKQLKEELLGIAPIFQQMPYFMNEEFGLVDCYVAPLLWKLKHLGVEFTGTGS
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCH
KAIKAYMERVFTRDSFLQSVGEAAPKNLMDDK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSASSKRSVMTLFSNKDDIYCHQVKIVLAEKGVLYENAEVDLQALPEDLMELNPYGTVP
CCCCHHHHHHHEECCCCCEEEEEEEEEEECCCEEECCCCCCHHHCCHHHHHCCCCCCCC
TLVDRDLVLFNSRIIMEYLDERFPHPPLMQVYPVSRAKDRLLMLRIEQDWYPTLAKAENG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHCCCC
TEKEKTSALKQLKEELLGIAPIFQQMPYFMNEEFGLVDCYVAPLLWKLKHLGVEFTGTGS
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCH
KAIKAYMERVFTRDSFLQSVGEAAPKNLMDDK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800