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The map label for this gene is cspD

Identifier: 16273340

GI number: 16273340

Start: 1522993

End: 1523211

Strand: Reverse

Name: cspD

Synonym: HI1434.1

Alternate gene names: 16273340

Gene position: 1523211-1522993 (Counterclockwise)

Preceding gene: 30995449

Following gene: 16273338

Centisome position: 83.23

GC content: 36.53

Gene sequence:

>219_bases
ATGGAAATTGGTATTGTAAAATGGTTCAATAATGCAAAAGGATTTGGTTTCATTTCCGCAGAAGGTGTAGATGCCGATAT
TTTTGCACATTATTCAGTGATTGAAATGGACGGCTATCGTTCATTAAAAGCGGGTCAAAAAGTACAATTTGAAGTTCTCC
ACAGCGATAAAGGATCTCACGCCACAAAAATAATCCCAATTGCGGATACTCAAGAATAA

Upstream 100 bases:

>100_bases
GYTCAMAATATTTAAACATAGCGAACCTAATTAGTAGGTAATAAGGCGAGAGTCTTGTTGATTTATTGTKTTAATAGGCC
GAAGCCTAGAGAGGTCTTTT

Downstream 100 bases:

>100_bases
TAAATACCCTAAATTTTTYCTATTAATCCTAACTTAAAAGACAAGAATTAAAAGTTAGAAAGTCAGTAAAGGACTAAGCT
TTGTGATTAAAAAAAGCTTA

Product: cold shock-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 72; Mature: 72

Protein sequence:

>72_residues
MEIGIVKWFNNAKGFGFISAEGVDADIFAHYSVIEMDGYRSLKAGQKVQFEVLHSDKGSHATKIIPIADTQE

Sequences:

>Translated_72_residues
MEIGIVKWFNNAKGFGFISAEGVDADIFAHYSVIEMDGYRSLKAGQKVQFEVLHSDKGSHATKIIPIADTQE
>Mature_72_residues
MEIGIVKWFNNAKGFGFISAEGVDADIFAHYSVIEMDGYRSLKAGQKVQFEVLHSDKGSHATKIIPIADTQE

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=68.6567164179104, Blast_Score=101, Evalue=1e-23,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=45.3125, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=48.4375, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=50, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=43.75, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=37.5, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=37.5, Blast_Score=59, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSPD_HAEIN (P46449)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_439584.1
- ProteinModelPortal:   P46449
- SMR:   P46449
- GeneID:   949903
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1434.1
- TIGR:   HI_1434.1
- HOGENOM:   HBG717773
- OMA:   HAVEIKD
- ProtClustDB:   CLSK870127
- BioCyc:   HINF71421:HI_1434.1-MONOMER
- GO:   GO:0005737
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- PRINTS:   PR00050
- SMART:   SM00357
- TIGRFAMs:   TIGR02381

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7958; Mature: 7958

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGIVKWFNNAKGFGFISAEGVDADIFAHYSVIEMDGYRSLKAGQKVQFEVLHSDKGSH
CCEEEEEEECCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
ATKIIPIADTQE
CEEEEEECCCCC
>Mature Secondary Structure
MEIGIVKWFNNAKGFGFISAEGVDADIFAHYSVIEMDGYRSLKAGQKVQFEVLHSDKGSH
CCEEEEEEECCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
ATKIIPIADTQE
CEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800