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The map label for this gene is glgX

Identifier: 16273268

GI number: 16273268

Start: 1439071

End: 1441050

Strand: Direct

Name: glgX

Synonym: HI1358

Alternate gene names: 16273268

Gene position: 1439071-1441050 (Clockwise)

Preceding gene: 16273267

Following gene: 16273269

Centisome position: 78.63

GC content: 41.46

Gene sequence:

>1980_bases
ATGTTCAAAATCTACAACAACGGCAATCCTATTCCAATGGGATACTCACAAGCGGTCGAAAACAACGTGCAAATTACCAA
TTTCGCCCTGTTTTCCGCTGCGGCAATAGGCGTAGAACTTTGCCTTTTTGACGAACAAAATCAAGAAACACGCTTGCCGA
TGGTGCGTACTGAAAATGTGTGGCATTTGGCGGTAACTGGCGTGAAAACTGGTACGGAATATGCTTTTCGTATTCACGGC
GAATTTGCCAATCCGCAGAAATTGATACTCGATCCCTATGCGAAAGCCGTGAATGGTAAACCTGATTTAAGTAGCGAAGA
AAGTCGTTCTTGGTTCTTGCTTTCGGATAATCGCGACAACGCGCATCTTGCCCCAAGAGCGGTTGTAATTTCAGAGGAAT
TTGATTGGGAAAATGACACTTCACCCAATACGCCTTGGGCTGAAACCATTGTGTATGAATTACATGTTAAAGGGTTTAGC
CAGTTAAACGAGAAAATCCCTGCGGCGTTGCGTGGTACTTATACTGGATTAGCACATCCTGTGAATTTGGCGTATTTGAA
AGAATTAGGCGTAACTGCGGTGGAGTTGCTGCCTGTAAATTTCCATATAAATGAACCGCACTTACAGGCACGAGGCTTAC
AAAATTATTGGGGATACAATCCCTTAGCGATGTTTGCGGTAGAACCTAAATATGCGGCAACAAATAATCCATTGGCTGAA
TTTAAAACGATGGTAAAGGCATTTCACAAAGCAGGCATTGAAGTTATTTTAGACGTGGTATTTAACCATTCCGCAGAATC
AGAGCAAACTTACCCAACATTCAGCCAGCGTGGTATTGATGATCAAACTTACTATTGGCGCAACGATCAAGGGCGTTATA
TCAATTGGACAGGCTGCGGCAATATGCTCAATTTATCCTCTGATGTAGGGCGAAAATGGGTGGTGGATTGCCTGCGTTAT
TGGGTGGAGCAATGCCATATTGATGGATTCCGTTTTGATTTAGCCACTGTTTTAGGGCGTGATACACCTGATTTCAACTC
GTCAGCTCAGCTTTTTACTGACATAAAAAATGAGCCAAGTTTACAAAATATTAAATTGATTGCCGAGCCGTGGGATATTG
GACATTACGGTTATCAAGTCGGCAATTTCCCAAGTTATTTTGCCGAATGGAACGACCGATTCCGTGATGATCTCTGCCGT
TTCTGGCTATGGAAAAGTGGCGAAATTGGTGCATTTGCAGAACGTTTTGCAGGTTCTAGCGATTTATTTAAGAAAAATGA
CCGCTTGCCACATACTACACTTAATTTTATTACCGCTCACGATGGTTTTACGCTTAAAGATTTAGTGAGTTATAACCAAA
AACATAACGAAACGAATGGCGAAGAAAATCGTGATGGTCGCAATGAAAATTACAGTTATAACCATGGAGTAGAAGGTTCT
ACAGAATCCCTTTCTGAACCGCAAAAAAGTGCGGTGGAAAATAACCGCACTTTTGCACAAAGTGGATTATTAATGAGTTT
ATTGCTGGCGAATGGTACACCTATGTTGTTAGCTGGCGATGAGTTCGGCAACACTCAATATGGTAACAATAATGCTTATT
GCCAAGATAACGAGATTACTTGGCTGAAATGGGCAAATTTTAACGAAGAATTATTTGAACTGACCAAACAAACGATTGCA
CTTCGTAAGCAAATTGGCAGTTTAAATAAAGATCAATGGTGGTCGGATGAGAATGTGCAATGGCTTAACATTGTAGGCGA
ACCGATGACAGTTGAAGATTGGCAAAATCAACAAACCAAAGCATTACAGGTGGTTTTAGACAATCGTTGGCTTTTGTTGA
TTAATGCCAAAGCCGAAGGTCAAATGTTTCACTTGCCTAATAGGAAATGGAAGCCCCAAATCGGCACACATAATGTAACC
CTTGAAGCACAACAAGCCGAACTTAGTTCAATGGGCTTTTGTATGCTGAATGACGAATAA

Upstream 100 bases:

>100_bases
AATAATCCGAATAGATAAATTGTAGGGGCGAATTACATTCGCCTGATAAACCACCGATGTTTTTGGGCAAATGTAATTTG
CCTACAAAATCGATAAAAAT

Downstream 100 bases:

>100_bases
CAATAAGACTATGGAGATCCTTATGAAATCTGGCGACCTTAACAAATATGATTTAGTTAAAAACGCTTTGGTACTTGTTC
TCGCAGGTGGACGTGGTTCA

Product: glycogen operon protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYAFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHKAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIVGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNRKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE

Sequences:

>Translated_659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYAFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHKAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIVGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNRKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE
>Mature_659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYAFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHKAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIVGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNRKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=667, Percent_Identity=50.2248875562219, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789839, Length=342, Percent_Identity=24.8538011695906, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGX_HAEIN (P45178)

Other databases:

- EMBL:   L42023
- PIR:   A64119
- RefSeq:   NP_439509.1
- ProteinModelPortal:   P45178
- SMR:   P45178
- GeneID:   949470
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1358
- NMPDR:   fig|71421.1.peg.1294
- TIGR:   HI_1358
- HOGENOM:   HBG350946
- OMA:   EMALDEF
- ProtClustDB:   CLSK870152
- BioCyc:   HINF71421:HI_1358-MONOMER
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02100

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75291; Mature: 75291

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: ACT_SITE 333-333 ACT_SITE 369-369

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAAIGVELCLFDEQNQETRLPMVRTENV
CEEEECCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHCEEEEEECCCCCCCCCCEEEECCE
WHLAVTGVKTGTEYAFRIHGEFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDN
EEEEEEEECCCCEEEEEEECCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEECCCCC
AHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCHHHCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKTMVKAFHKAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNKDQWWSDENVQWLNIVGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQMFHLPNRKWKPQIGTHNVTLEAQQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSAAAIGVELCLFDEQNQETRLPMVRTENV
CEEEECCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHCEEEEEECCCCCCCCCCEEEECCE
WHLAVTGVKTGTEYAFRIHGEFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDN
EEEEEEEECCCCEEEEEEECCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEECCCCC
AHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCHHHCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKTMVKAFHKAGIEVILDVVFNHSAESEQTYPTFSQRGIDDQTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNKDQWWSDENVQWLNIVGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQMFHLPNRKWKPQIGTHNVTLEAQQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800