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The map label for this gene is pyrF

Identifier: 16273143

GI number: 16273143

Start: 1293871

End: 1294563

Strand: Direct

Name: pyrF

Synonym: HI1224

Alternate gene names: 16273143

Gene position: 1293871-1294563 (Clockwise)

Preceding gene: 16273142

Following gene: 16273144

Centisome position: 70.7

GC content: 39.54

Gene sequence:

>693_bases
ATGACAAGTAAAATTATTGTGGCATTGGATTTCGAGAAAGAAGCAGAGGCTCTTGCTTTAGTTGATCAAATTGATCCAAG
TTTGTGTCGATTAAAAGTGGGTAAAGAAATGTTTACTACACTTGGTATTAATTTTGTAAAACAATTACATCAACGTAATT
TTGATGTTTTTTTGGATCTGAAATATCACGATATTCCAAATACGGTAGCAAGAGCAGTTCGTTCTGCTGCTGATTTAGGC
GTATGGATGGTGGATTTACACGCAAGTGGTGGCTTGCGTATGATGGAAGACGCAAAGAAAATTCTTGAACCTTATGGGAA
AGATGCGCCATTGCTTATTGCTGTGACGGTATTAACCAGTATGGAAGACTTGGATTTATTACAAATTGGGATTAATGCAT
CGCCAATGGAACAAGTTTTACGCTTAGCTCATTTAACCCAACGGGCAGGTTTGGATGGTGTGGTTTGCTCTCCGCAAGAA
GTTGAAATTTTACGTAATGCTTGTGGAGAAGACTTTAAATTAGTGACCCCAGGGATTCGTCCAATAGGAACTGATTTTGG
CGATCAACGACGTGTAATGACACCTACCGCAGCAATTCGTGCTGGTTCAGATTATTTAGTTATTGGACGCCCAATAACTC
AAGCGGATAATCCTGCGGAGGTTCTCCGTTCTATTAATGTATCTATTGGGTAA

Upstream 100 bases:

>100_bases
ATAAGCTATTGTGGAATTGCCCATCTTGCCGTCAATGGGAAAGTATTAAACCGGTTTCAAATCAAGAACATAATTAATTG
ACACTCAGTGAGGTAACATT

Downstream 100 bases:

>100_bases
TCATAAATGTCAGATTCTGTTTTAGTGTATTCAACAGATGTGGGTCGAATTAAAGAGGAAAAGGCTTCTGTAGTTCGCCC
AAAAGGAGACGGTGTTGTAC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTSKIIVALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQE
VEILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Sequences:

>Translated_230_residues
MTSKIIVALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQE
VEILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
>Mature_229_residues
TSKIIVALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLGV
WMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEV
EILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=62.280701754386, Blast_Score=291, Evalue=2e-80,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_HAEIN (P43812)

Other databases:

- EMBL:   L42023
- PIR:   B64111
- RefSeq:   NP_439380.1
- ProteinModelPortal:   P43812
- SMR:   P43812
- GeneID:   950791
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1224
- TIGR:   HI_1224
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   HINF71421:HI_1224-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25193; Mature: 25061

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 119-119 BINDING 180-180 BINDING 189-189 BINDING 209-209 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKIIVALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDL
CCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHC
MEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCC
PIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCCCCCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSKIIVALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDL
CCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHC
MEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCC
PIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCCCCCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800