Click here to switch to the map view.

The map label for this gene is rfaF

Identifier: 16273031

GI number: 16273031

Start: 1168738

End: 1169778

Strand: Reverse

Name: rfaF

Synonym: HI1105

Alternate gene names: 16273031

Gene position: 1169778-1168738 (Counterclockwise)

Preceding gene: 16273032

Following gene: 16273027

Centisome position: 63.92

GC content: 40.73

Gene sequence:

>1041_bases
ATGAATATTCTAATTATTGGCCCATCTTGGGTTGGCGATATGATGATGTCGCACAGCTTGTACCAGCAACTGAAAATTCA
ATATCCAAACTGCAATATTGATGTGATGGCACCGAATTGGTGTAAGCCTTTGCTTGCGCGAATGCCAGAGGTGCGTAAGG
CGATTGAAATGCCATTAGGGCACGGGGCCTTTGAATTGGGAACGCGTTATCGTTTAGGAAAGTCTTTGCGTGAACAATAT
GATATGGCAATTGTTTTGCCTAATTCATTGAAATCGGCGTTTATTCCTTTCTTCGCAAAAATTGTCCATCGTCGCGGTTG
GAAAGGCGAAAGCCGTTATATTTTATTGAATGATTTACGAGCCAATAAAAAAGATTATCCAATGATGGTGCAGCGTTATG
TTGCATTAGCTTTTGAAAAAGATGTTATACCAAAAGCTGATGATATTCCTGTCTTGAAACCCTATTTGACGGTTGAGCCA
GCGCAACAAGCTGAAACCTTAAAAAAATTTGAAAAACAAACCGCACTTTTAGGCGAGCGTCCAATTATTGGTTTTTGTCC
TGGTGCGGAATTTGGCCCAGCAAAACGTTGGCCACATTATCATTATGCAAAATTAGCAGAAATGTTAATTACCCAAGGCT
ATGCTGTGGCGCTATTTGGTTCTGCTAAAGATGAACCTGTGGGCGAAGAAATTCGTCAAGCGCTGCCAGAAGAGTTGCGT
GAGTTTTGTGTCAATTTAGCAGGAAAAACCAACTTGAATGAAGCGGTGGATTTAATTGCAAATTGTACGGCAGTCGTGAC
CAATGACAGTGGTTTAATGCATATTGCGGCAGCAGTAAATCGTCCATTGATTGCACTTTATGGACCAACTAGCCCACAAT
ATACGCCGCCGCTTTCAGATAAAGCAACGATTATTCGTTTAATTGAAGGTGAATTAATTAAAGTCCGTAAAGGCGACAAA
GAGGGTGGGTATCATCAAAGTTTGATTGATATTACGCCAGAAATGGCATTAGAAAAATTGAACGAACTGTTGGCAAAATA
A

Upstream 100 bases:

>100_bases
TAAAGAGTTTCGACTAAATTGTTAAAGGAGCATATTTTTATTCAACTCTGTGCCATAATAACAAGTATTTTTGACCGCAC
TTTTATCTGAAAGACAGAAA

Downstream 100 bases:

>100_bases
TGAAAGTTTGCTTAGTGAAAAGAAAACTTATAAAGATATTACTTATAAGATGACTTGTTTCATAAAAAATGCTCGCACTT
GGCGAACATTTTTATCTTTT

Product: ADP-heptose-LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKSLREQY
DMAIVLPNSLKSAFIPFFAKIVHRRGWKGESRYILLNDLRANKKDYPMMVQRYVALAFEKDVIPKADDIPVLKPYLTVEP
AQQAETLKKFEKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEELR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGELIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK

Sequences:

>Translated_346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKSLREQY
DMAIVLPNSLKSAFIPFFAKIVHRRGWKGESRYILLNDLRANKKDYPMMVQRYVALAFEKDVIPKADDIPVLKPYLTVEP
AQQAETLKKFEKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEELR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGELIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK
>Mature_346_residues
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLGHGAFELGTRYRLGKSLREQY
DMAIVLPNSLKSAFIPFFAKIVHRRGWKGESRYILLNDLRANKKDYPMMVQRYVALAFEKDVIPKADDIPVLKPYLTVEP
AQQAETLKKFEKQTALLGERPIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEELR
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSDKATIIRLIEGELIKVRKGDK
EGGYHQSLIDITPEMALEKLNELLAK

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family

Homologues:

Organism=Escherichia coli, GI1790050, Length=348, Percent_Identity=63.2183908045977, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAF_HAEIN (P45042)

Other databases:

- EMBL:   L42023
- PIR:   G64182
- RefSeq:   NP_439262.1
- ProteinModelPortal:   P45042
- SMR:   P45042
- GeneID:   950079
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1105
- NMPDR:   fig|71421.1.peg.1062
- TIGR:   HI_1105
- HOGENOM:   HBG527482
- OMA:   MWIGDFV
- ProtClustDB:   CLSK870635
- BioCyc:   HINF71421:HI_1105-MONOMER
- InterPro:   IPR002201
- InterPro:   IPR011910
- TIGRFAMs:   TIGR02195

Pfam domain/function: PF01075 Glyco_transf_9

EC number: 2.4.1.-

Molecular weight: Translated: 38848; Mature: 38848

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLG
CEEEEECCHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHCHHHHHHHHCCCC
HGAFELGTRYRLGKSLREQYDMAIVLPNSLKSAFIPFFAKIVHRRGWKGESRYILLNDLR
CCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ANKKDYPMMVQRYVALAFEKDVIPKADDIPVLKPYLTVEPAQQAETLKKFEKQTALLGER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCC
PIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEELR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSD
HHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCCCCCCCCC
KATIIRLIEGELIKVRKGDKEGGYHQSLIDITPEMALEKLNELLAK
CHHEEEECCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNILIIGPSWVGDMMMSHSLYQQLKIQYPNCNIDVMAPNWCKPLLARMPEVRKAIEMPLG
CEEEEECCHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHCHHHHHHHHCCCC
HGAFELGTRYRLGKSLREQYDMAIVLPNSLKSAFIPFFAKIVHRRGWKGESRYILLNDLR
CCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ANKKDYPMMVQRYVALAFEKDVIPKADDIPVLKPYLTVEPAQQAETLKKFEKQTALLGER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCC
PIIGFCPGAEFGPAKRWPHYHYAKLAEMLITQGYAVALFGSAKDEPVGEEIRQALPEELR
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EFCVNLAGKTNLNEAVDLIANCTAVVTNDSGLMHIAAAVNRPLIALYGPTSPQYTPPLSD
HHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCCCCCCCCC
KATIIRLIEGELIKVRKGDKEGGYHQSLIDITPEMALEKLNELLAK
CHHEEEECCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800