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The map label for this gene is ispH [H]

Identifier: 16272942

GI number: 16272942

Start: 1071996

End: 1072940

Strand: Direct

Name: ispH [H]

Synonym: HI1007

Alternate gene names: 16272942

Gene position: 1071996-1072940 (Clockwise)

Preceding gene: 16272941

Following gene: 16272943

Centisome position: 58.57

GC content: 40.74

Gene sequence:

>945_bases
ATGAAGATAATTTTAGCCAATCCACGAGGATTTTGTGCCGGTGTAGATCGTGCCATTAGTATTGTTGAATTAGCCTTAGA
AATTCACGGTGCGCCTATTTATGTTCGTCACGAAGTGGTACATAACCGATTTGTTGTGAACGGATTGCGTGAGCGTGGTG
CGATCTTTGTGGAAGAATTAAGCGAGGTGCCTGATGGTGCGATCGTGATTTTTTCTGCACACGGTGTGTCTCAAGCGGTT
CGGCAAGAAGCAAAAGATCGTAATCTCAAAGTATTTGATGCAACCTGTCCTTTGGTGACTAAAGTGCATATGCAGGTTGC
GCGCGCAAGTCGAAAGGGTACAAAAGCGATTTTGATTGGGCATAAAGGGCATCCTGAAGTTGAAGGCACAATGGGGCAGT
ATAGCAATGAAGATGGCGGTATTTTCCTAATTGAAAAAGTAGAAGATATTGCGCGTTTGCCAATGCAAGAAAATGATAAT
TTAACCTTTATGACGCAAACGACGTTGTCGCTAGATGATACGGCTGAAACTATTGCTGCATTAAAGGAAAAATATCCCGC
TATTCAAGGACCACATAAAAACGATATTTGTTATGCGACAACCAATCGTCAAGAAGCGGTGCGTGAATTAGCAAAATTAT
CAGATTTAGTGTTGGTTGTAGGTTCTAAAAATTCATCCAATTCAAATCGTTTAGCTGAATTGGCTTCTCGAATGGGGGTT
AAATCACAGCTTCTTGATGAACCTGCCGATATTCAAGCTGATTGGTTTAATGATGTAAAAACCATTGGGATTACAGCTGG
TGCTTCTGCGCCAGAGGAATTAGGTCAATCGATTATTTCTCGTTTAAAAAGGTTTGGTGCGAATTCCATAGAAGAACTGC
AAGGTTTAGAAGGAAATATGTTCTTTGAGGTGCCAAAAGAGCTGAGAATAAAAGAAGTTAATTAA

Upstream 100 bases:

>100_bases
ATATCGCCATTTGTATTGGTGCAGGGTTATTAGTATTAGATGCGTTTAAGAGCGAAAAGAAAAAAGTACAAGATAAACAA
GTAGAAAAGTGCGGTCAGAA

Downstream 100 bases:

>100_bases
TACTAAGGGCTTGTCGTAAATGATAAGCCCTTTGTTTTTTCATTCAAATTAAGAATTTTTGCGATCGAGATCGCAAAAGT
GCGGTCAGTTTCTTGAGGGT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKIILANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVNGLRERGAIFVEELSEVPDGAIVIFSAHGVSQAV
RQEAKDRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHKGHPEVEGTMGQYSNEDGGIFLIEKVEDIARLPMQENDN
LTFMTQTTLSLDDTAETIAALKEKYPAIQGPHKNDICYATTNRQEAVRELAKLSDLVLVVGSKNSSNSNRLAELASRMGV
KSQLLDEPADIQADWFNDVKTIGITAGASAPEELGQSIISRLKRFGANSIEELQGLEGNMFFEVPKELRIKEVN

Sequences:

>Translated_314_residues
MKIILANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVNGLRERGAIFVEELSEVPDGAIVIFSAHGVSQAV
RQEAKDRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHKGHPEVEGTMGQYSNEDGGIFLIEKVEDIARLPMQENDN
LTFMTQTTLSLDDTAETIAALKEKYPAIQGPHKNDICYATTNRQEAVRELAKLSDLVLVVGSKNSSNSNRLAELASRMGV
KSQLLDEPADIQADWFNDVKTIGITAGASAPEELGQSIISRLKRFGANSIEELQGLEGNMFFEVPKELRIKEVN
>Mature_314_residues
MKIILANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVNGLRERGAIFVEELSEVPDGAIVIFSAHGVSQAV
RQEAKDRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHKGHPEVEGTMGQYSNEDGGIFLIEKVEDIARLPMQENDN
LTFMTQTTLSLDDTAETIAALKEKYPAIQGPHKNDICYATTNRQEAVRELAKLSDLVLVVGSKNSSNSNRLAELASRMGV
KSQLLDEPADIQADWFNDVKTIGITAGASAPEELGQSIISRLKRFGANSIEELQGLEGNMFFEVPKELRIKEVN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=67.7215189873418, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34469; Mature: 34469

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIILANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVNGLRERGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
SEVPDGAIVIFSAHGVSQAVRQEAKDRNLKVFDATCPLVTKVHMQVARASRKGTKAILIG
HHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HKGHPEVEGTMGQYSNEDGGIFLIEKVEDIARLPMQENDNLTFMTQTTLSLDDTAETIAA
CCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHH
LKEKYPAIQGPHKNDICYATTNRQEAVRELAKLSDLVLVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCC
KSQLLDEPADIQADWFNDVKTIGITAGASAPEELGQSIISRLKRFGANSIEELQGLEGNM
HHHHHCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCE
FFEVPKELRIKEVN
EEECCCCCEEEECC
>Mature Secondary Structure
MKIILANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVNGLRERGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
SEVPDGAIVIFSAHGVSQAVRQEAKDRNLKVFDATCPLVTKVHMQVARASRKGTKAILIG
HHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HKGHPEVEGTMGQYSNEDGGIFLIEKVEDIARLPMQENDNLTFMTQTTLSLDDTAETIAA
CCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHH
LKEKYPAIQGPHKNDICYATTNRQEAVRELAKLSDLVLVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCC
KSQLLDEPADIQADWFNDVKTIGITAGASAPEELGQSIISRLKRFGANSIEELQGLEGNM
HHHHHCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCE
FFEVPKELRIKEVN
EEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA