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The map label for this gene is dusB

Identifier: 16272917

GI number: 16272917

Start: 1037121

End: 1038113

Strand: Direct

Name: dusB

Synonym: HI0979

Alternate gene names: 16272917

Gene position: 1037121-1038113 (Clockwise)

Preceding gene: 16272916

Following gene: 16272918

Centisome position: 56.67

GC content: 40.89

Gene sequence:

>993_bases
ATGCGAATCGGTTCATACCAATTAAGAAATCGTGTTTTACTTGCACCAATGGCAGGTATTACCGACCAGCCTTTCCGCCG
TCTTTGTGCATATTATGGTGCGGGGCTTACATTTTCAGAAATGATGTCCACAAATCCGCAAGTTTGGCATACCGAAAAAT
CCAAATTGCGTTTGGCACATAGCGAAGATTTAGGTCTCAATGCGGTGCAAATTGCAGGTAGTGATCCACTCGAGATGGCG
CAAGCCGCTGCTATTAACGTGGAATATGGCGCTCAAATCATTGACATTAATATGGGATGCCCTGCCAAAAAAGTAAATCG
CAAACTCGCAGGTTCTGCGTTGTTGCAATTTCCAGATTTGGTGGAAAAAATCTTGCGAGAAGTAGTGTCCGCCGTCAATG
TGCCAGTGACCTTAAAAATTCGTACAGGCTGGGATAAATCTAATCGAAACTGCGTTCAAATCGGCAAAATTGCAGAACAA
TGTGGCATACAAGCATTAACGGTGCATGGTCGTACACGCGCTTGTCTTTTTGAAGGCGAAGCGGAGTACGATAACATTAA
AGCCGTTAAACAAGCAATTGCTATACCAGTTATAGCTAATGGCGATATTGATTCTGCTCGGAAAGCAAAATTCGTTCTCA
ATTATACTGGAGCGGATGCCATTATGATCGGTCGTGCTGCCTTAGGTAACCCTTGGTTATTTCAAGCTGTGGAAAACCTA
ATTGAGCATAACTCGATAAGTCAAATGCCAAGTTTAAAAGAAAAGTGTGGTCAGATTTTGCGTCATATTCAAGAATTGCA
TCAATTCTATGGCGAACAAAAAGGCTATCGCATTGCGCGTAAACACGTAGCTTGGTATTTACAGGGAATTCAACCTGATT
CCGTTTTTAAACAGACTTTTAATGCAATTAGTGATCCTAAAGAGCAGCTGATTGTATTGGAAGATTTTTTCAATTTAATT
TTGGATAAAAAAAATGTTAGAACAACAACGTAA

Upstream 100 bases:

>100_bases
TTGCAAAGCAAGGCAAAATGCCGTATTAATAGCCAGCCTTGTCGGTTGTCGAGCAGTTCGATATAAATTAGATTTAGGCT
TGCTAGGAAAGGAACGTAAA

Downstream 100 bases:

>100_bases
TTCTGCCGATGCCTTAACGGTATCAGTATTAAATGCGCAATCACAAGTGACCAGCAAACCATTACGCGATTCAGTCAAAC
AAGCGTTGCGTAATTATTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRIGSYQLRNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPDLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLNYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKKNVRTTT

Sequences:

>Translated_330_residues
MRIGSYQLRNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPDLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLNYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKKNVRTTT
>Mature_330_residues
MRIGSYQLRNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAHSEDLGLNAVQIAGSDPLEMA
QAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPDLVEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQ
CGIQALTVHGRTRACLFEGEAEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLNYTGADAIMIGRAALGNPWLFQAVENL
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTFNAISDPKEQLIVLEDFFNLI
LDKKNVRTTT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=33.1932773109244, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=229, Percent_Identity=33.6244541484716, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=33.1932773109244, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=33.4661354581673, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.9055793991416, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=65.615141955836, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=36.8200836820084, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI145693211, Length=248, Percent_Identity=28.6290322580645, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=221, Percent_Identity=33.4841628959276, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=246, Percent_Identity=31.7073170731707, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=263, Percent_Identity=32.319391634981, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=31.4159292035398, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=315, Percent_Identity=25.0793650793651, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=263, Percent_Identity=28.1368821292776, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=244, Percent_Identity=31.9672131147541, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=34.1880341880342, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=34.1880341880342, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=30.8370044052863, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45549423, Length=293, Percent_Identity=30.3754266211604, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSB_HAEIN (P44965)

Other databases:

- EMBL:   L42023
- PIR:   C64163
- RefSeq:   NP_439142.1
- ProteinModelPortal:   P44965
- SMR:   P44965
- GeneID:   949982
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0979
- NMPDR:   fig|71421.1.peg.946
- TIGR:   HI_0979
- HOGENOM:   HBG557545
- OMA:   QLAGCEP
- ProtClustDB:   CLSK870359
- BioCyc:   HINF71421:HI_0979-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 36765; Mature: 36765

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGSYQLRNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAH
CCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHEEEEC
SEDLGLNAVQIAGSDPLEMAQAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPDL
CCCCCCEEEEECCCCHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQCGIQALTVHGRTRACLFEGE
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLNYTGADAIMIGRAALGNPWLFQAVENL
CCHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEECCCCEEEECCHHCCCCHHHHHHHHH
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTF
HHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NAISDPKEQLIVLEDFFNLILDKKNVRTTT
HHHCCHHHHEEEHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRIGSYQLRNRVLLAPMAGITDQPFRRLCAYYGAGLTFSEMMSTNPQVWHTEKSKLRLAH
CCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHEEEEC
SEDLGLNAVQIAGSDPLEMAQAAAINVEYGAQIIDINMGCPAKKVNRKLAGSALLQFPDL
CCCCCCEEEEECCCCHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VEKILREVVSAVNVPVTLKIRTGWDKSNRNCVQIGKIAEQCGIQALTVHGRTRACLFEGE
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDNIKAVKQAIAIPVIANGDIDSARKAKFVLNYTGADAIMIGRAALGNPWLFQAVENL
CCHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEECCCCEEEECCHHCCCCHHHHHHHHH
IEHNSISQMPSLKEKCGQILRHIQELHQFYGEQKGYRIARKHVAWYLQGIQPDSVFKQTF
HHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NAISDPKEQLIVLEDFFNLILDKKNVRTTT
HHHCCHHHHEEEHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800