Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 16272869

GI number: 16272869

Start: 989924

End: 991234

Strand: Reverse

Name: eno [H]

Synonym: HI0932

Alternate gene names: 16272869

Gene position: 991234-989924 (Counterclockwise)

Preceding gene: 16272870

Following gene: 30995410

Centisome position: 54.16

GC content: 41.71

Gene sequence:

>1311_bases
ATGGCAAAAATCGTTAAAGTGATTGGTCGCGAAATCATCGACTCACGCGGTAATCCAACTGTTGAAGCTGAAGTTCATCT
TGAKGGTGGTTTTGTNGGTTTAGCAGCAGCTCCATCTGGTGCATCAACTGGTTCTCGTGAAGCATTAGAATTACGTGACG
GCGACAAATCTCGTTTCTTAGGTAAAGGCGTATTAAAAGCTGTGGCGGCAGTGAACAACGAAATTGCACAAGCTATCGTT
GGTAAAGATGCAACAAACCAAGCTGAAATCGACCAAATCATGATCGATTTAGACGGAACCGAAAACAAATCTAACTTTGG
TGCAAATGCAATCTTGGCGGTATCTTTAGCAAACGCAAAAGCAGCTGCAGCATCTAAAGGTTTACCACTTTACGCTTACA
TTGCAGAATTAAATGGCACTGCTGGTGTTTATTCTATGCCATTACCAATGATGAACATCATTAACGGTGGCGAACATGCA
GATAACAACGTTGATATCCAAGAATTCATGATTCAACCAGTTGGTGCGAAAACATTACGTGAAGCACTTCGTATCGGTGC
TGAAGTATTCCACAACTTGGCGAAAGTATTAAAAGCTAAAGGCATGAGCACTGCAGTTGGTGACGAAGGTGGTTTCGCAC
CAAACTTAGCCTCTAACGCAGACGCTTTAGCCTGTATCAAAGAAGCAGTAGAAAAAGCAGGTTACGTATTAGGTAAAGAC
GTTACTTTAGCGATGGACTGCGCATCTTCTGAGTTCTATAACAAAGAAAATGGTATGTACGAAATGAAAGGTGAAGGTAA
ATCATTCACTTCTCAAGAATTCACTCACTACTTAGAAGAATTATGCAAAGAGTACCCAATCGTGTCTATCGAAGATGGTC
AAGATGAGTCAGACTGGGAAGGTTTTGCATACCAAACTAAAGTGTTAGGCGACCGCGTTCAATTAGTGGGCGATGATTTA
TTCGTCACTAACACCAAAATCTTAAAAGAAGGTATCGAAAAAGGTATCGCAAACTCTATCTTAATCAAATTCAATCAAAT
CGGTTCTTTAACTGAAACTTTAGCAGCAATTAAAATGGCGAAAGATGCAGGTTACACCGCTGTAATCTCTCACCGTTCAG
GCGAAACTGAAGATGCAACTATCGCTGATTTAGCGGTGGGTACAGCAGCAGGTCAAATCAAAACTGGTTCTATGAGCCGT
TCTGACCGTATTGCGAAATACAACCAATTAATCCGTATCGAAGAAGCATTAGAACGCGCTGGTACTCCAGCAGCATTCCC
AGGCTTAAAAGCGGTTAAAGGTCAAGCGTAA

Upstream 100 bases:

>100_bases
ACGCATTTTTTACAAGAAAAAATCTRAAAATAGCTTTATCATATCGCCTGTTTTAGGCTCGCCTAACCCGTTTTAATTAA
CTTAATTGAGGAAAATAAAA

Downstream 100 bases:

>100_bases
TTCACGCTGAATTTACTGTGAAATCTCACCGCACTTTGTATAAAAGTGCGGTGTTTTTATATCTAATATTTAAAGGAAAG
AGAATGTTACATTTAACTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLXGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIV
GKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA

Sequences:

>Translated_436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLXGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIV
GKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA
>Mature_435_residues
AKIVKVIGREIIDSRGNPTVEAEVHLXGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNNEIAQAIVG
KDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLREALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKDV
TLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDRVQLVGDDLF
VTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRIAKYNQLIRIEEALERAGTPAAFPGLKAVKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=52.3041474654378, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=52.3041474654378, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=50.9216589861751, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.3824884792627, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.4537037037037, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1789141, Length=437, Percent_Identity=84.4393592677345, Blast_Score=723, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=425, Percent_Identity=53.1764705882353, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=436, Percent_Identity=52.5229357798165, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=195, Percent_Identity=45.6410256410256, Blast_Score=168, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=49.0825688073394, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323985, Length=425, Percent_Identity=48, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324974, Length=425, Percent_Identity=48, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=425, Percent_Identity=48, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=48.1651376146789, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=51.4150943396226, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=51.4150943396226, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=51.4150943396226, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=51.4150943396226, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=418, Percent_Identity=51.9138755980861, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=418, Percent_Identity=51.9138755980861, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46061; Mature: 45930

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVKVIGREIIDSRGNPTVEAEVHLXGGFVGLAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQAIVGKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLR
HHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDRVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CCEEHHHHHCCHHHEECCCEEEEHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALERA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAAFPGLKAVKGQA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKIVKVIGREIIDSRGNPTVEAEVHLXGGFVGLAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQAIVGKDATNQAEIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAASKGLPLYAYIAELNGTAGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLR
HHHHCCCCCEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKAKGMSTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDRVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CCEEHHHHHCCHHHEECCCEEEEHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALERA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAAFPGLKAVKGQA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA