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The map label for this gene is proB [H]

Identifier: 16272838

GI number: 16272838

Start: 956115

End: 957221

Strand: Direct

Name: proB [H]

Synonym: HI0900

Alternate gene names: 16272838

Gene position: 956115-957221 (Clockwise)

Preceding gene: 16272834

Following gene: 16272839

Centisome position: 52.24

GC content: 39.93

Gene sequence:

>1107_bases
ATGAACAAAAAAACAATCGTTGTGAAATTCGGTACAAGCACGCTAACTCAGGGTTCGCCAAAACTGAATTCGCCACATAT
GATGGAAATCGTTCGTCAAATTGCACAACTGCACAATGATGGATTTCGTATAGTGATCGTGACTTCTGGTGCGATTGCAG
CGGGTCGCCATTATTTAAATCATCCTCAATTACCACCCACTATCGCCTCAAAGCAACTGCTTGCAGCGGTTGGTCAGAGC
CAATTAATTCAGGCTTGGGAAAAATTATTTGCTATTTATGATATCCATATCGGGCAACTTTTATTAACCCGTGCAGATAT
TGAAGATCGCGAACGTTTTTTAAATGCGCGAGATACTTTATACGCACTTTTAGATAACCACATTATTCCTGTGATTAATG
AAAATGATGCGGTAGCGACTGCGGAAATTAAAGTAGGAGACAATGACAATTTATCGGCATTAGTCGCTATTCTTGTGCAA
GCGGAACAGCTTTATTTATTGACTGATCAACAAGGTTTATTTGATAGTGATCCACGTAAAAATCCTGAAGCGAAATTAAT
TCCAGTTGTAGAACAAATCACCGATCATATTCGATCAATTGCAGGAGGAAGTGGTACAAATCTTGGTACTGGTGGAATGA
TGACCAAAATCATTGCTGCCGATGTTGCGACTCGCTCTGGAATTGAAACCATTATTGCTCCAGGTAATCGCCCAAATGTA
ATTGCAGATCTTGCTTATGAACAAAATATTGGTACAAAATTTATTGCACACCAATCTGATCGATTAGAAAGCCGCAAACA
ATGGCTTTTCGCCGCACCATCGGCAGGTATTATTACCATTGATAACGGTGCGCAAAATGCCATTCTTGAACAAAATAAAT
CTTTATTACCCGCTGGTATTATTAACGTGGAAGGGCGTTTTTCGCGTGGTGAAGTAGTCAAAATACGCACTCAATCAGGT
AAAGACATTGCGCTGGGTATGCCACGCTATAACAGCGATGCTCTACAATTAATTAAGGGCAGAAAATCAGCAGACATTGA
GAATGTTTTAGGCTATGAATATGGCGCAGTGGCAATGCATCGTGATGATATGATTATTTTGTCATAA

Upstream 100 bases:

>100_bases
AAATCCCCCTAAAATTTGCCGTTACTATACCTGATATTAAGTGATTTTTCATTGATTTAGTGATAACGTTAATGCCAATT
TTAAGAACTTGGAAAGGATT

Downstream 100 bases:

>100_bases
TGTCCTTTGGTTGATAAAAGCACTGATTTATGAAACAATCTTGAGCTATTTTTATAGGAAAAATTTAAGGTAAAACGATA
GTGATCGATTTTGATGGCTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLYALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNPEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDALQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS

Sequences:

>Translated_368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLYALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNPEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDALQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS
>Mature_368_residues
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQS
QLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTLYALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQ
AEQLYLLTDQQGLFDSDPRKNPEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGIINVEGRFSRGEVVKIRTQSG
KDIALGMPRYNSDALQLIKGRKSADIENVLGYEYGAVAMHRDDMIILS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=33.2167832167832, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=33.2167832167832, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=65.9340659340659, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17569967, Length=266, Percent_Identity=31.9548872180451, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=266, Percent_Identity=31.9548872180451, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=36.3855421686747, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=35.7487922705314, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI21357643, Length=284, Percent_Identity=31.6901408450704, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40135; Mature: 40135

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLN
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHCHHHCC
HPQLPPTIASKQLLAAVGQSQLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTL
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDSDPRK
HHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCC
NPEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCE
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGI
EEEEEECCCCCCEEEECCHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHCCCCCCCEEE
INVEGRFSRGEVVKIRTQSGKDIALGMPRYNSDALQLIKGRKSADIENVLGYEYGAVAMH
EEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEE
RDDMIILS
CCCEEEEC
>Mature Secondary Structure
MNKKTIVVKFGTSTLTQGSPKLNSPHMMEIVRQIAQLHNDGFRIVIVTSGAIAAGRHYLN
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHCHHHCC
HPQLPPTIASKQLLAAVGQSQLIQAWEKLFAIYDIHIGQLLLTRADIEDRERFLNARDTL
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YALLDNHIIPVINENDAVATAEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDSDPRK
HHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCC
NPEAKLIPVVEQITDHIRSIAGGSGTNLGTGGMMTKIIAADVATRSGIETIIAPGNRPNV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCE
IADLAYEQNIGTKFIAHQSDRLESRKQWLFAAPSAGIITIDNGAQNAILEQNKSLLPAGI
EEEEEECCCCCCEEEECCHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHCCCCCCCEEE
INVEGRFSRGEVVKIRTQSGKDIALGMPRYNSDALQLIKGRKSADIENVLGYEYGAVAMH
EEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEE
RDDMIILS
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA