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The map label for this gene is slt

Identifier: 16272770

GI number: 16272770

Start: 879389

End: 881170

Strand: Direct

Name: slt

Synonym: HI0829

Alternate gene names: 16272770

Gene position: 879389-881170 (Clockwise)

Preceding gene: 16272769

Following gene: 16272771

Centisome position: 48.05

GC content: 35.58

Gene sequence:

>1782_bases
ATGAAAAAAGTCGCCTTAATTTCTCTCTGTATTTTTACCGCACTTTCTGCTTTTGCCGATTCGCCTAATACGGCAACAGC
ATCAATCAATCTTGAGCAAGAGAAACAAAACTGGGAGCTGGCGCAGCATCAAGATTATTTAAAACGTCTAAAACAACGTG
AAGTCTTTTTGCAAGTTGAAGGTTTGCTAAAAAGTGCGGTAAAAAAACAGCAATTTTCTGAAGCGACTCAAAATATCACA
AAAACCTTAATAGACTCATTGCAAGGCTACCCTTTGCAATATGATCTGCTTGCTCGATTTTGGGAGACAAAAATCGCCTT
TTTGCAGAATGATGATATTCAAGGAAGACAACAAGCAATAAATGAACTCAATGCGTTAGTGCAACAAAATTACCCTTTTG
TGACGCCAGCATTTCAAGCCTTATTACAAAAACTATCCACGTTAAACGAACAACAAACATCAGCAACTTCAGATAATGCT
AAAGAAAATAACAGGGTTCAAAAAGAGCAAAATCAAGTAGAAAATCCTAAACAACTTGCTGAAATTGTAAGAAAAAGTGA
TCCTAACACTTTAGATAAAACCGTATTAATTGATGCTTTCCCACGTTATCTTAAAACGCTACCTGAACAAATGAATAACC
TAAGTTTTGAATCCTATCAAAAATGGGCAAATACTTGGCAACTCTCAGAAGATGAAATTAAACAGTGGAAAATAGCCTTT
TTAAATCGTTTTTTTGATAATGAAAACACTGATTTTCAAAAATGGCGTGATGAACAAATCCGACAATTACAGACTGATAA
TTTAACCGAACGCCGTTTACGAATGGCAATTTGGCAAAAAACTGAATTAACCTCTTGGCTAAATTTGCTTTCTGCCGAAT
CAAAAAGTAAGCAAGAATGGCGTTATTGGGAAGCTAAACAAGATATATTAAAAAATACCAAAAAACTGACCGCACTTTCA
AAAGAGCGTGGCTTTTATCCAATGTTAGCGGCTACACAATTAAAACAAGCATATCAACTGAATGTCCCTATTGCGCCAAG
TTTTACTCAAGCTGAACAACTTCCTTTTAAGCAAGTTTTTGCAATGATTACAGAATTAAGAGAACTTGGACGAAATGGTT
TAGCAAAACAACGCTGGCGAATTTTACTTGATAATGTTGATTTCACAACTCAGCTAAAACTTTCTGAATATGCCAAAAAT
CAGCAATGGTTTGAATTAGCTGTTGATGCTTCTATTGTTGCAAAAGCGTGGGATTACCTGTCTCTTCGTCTGCCAAATGC
TTATTCTGAATACTTCAATGCTGCATTACAGAATTTGAATATCAGCAAAACTTTTGCTATGGCAATCGCTCGTCAAGAAA
GTGCTTGGAATCCAATGGCACAATCTTCAGCAAATGCTCGAGGCTTAATGCAGCTTTTGCCAAGTACGGCAAAATTGACT
GCAGAGAATAATCAACTACCTTATCAAGGCGAACAAGATTTATTCAAACCATTGAATAATATTTTGCTAGGTACCGCACA
TCTTAATGAACTCAATGGAAAATATCCTAATAATCGAATATTGATTGCAGCCGCTTATAATGCAGGGGCAAATCGAGTAG
GAAAATGGTTAAGTCGTGCGAGTGGAAAATTAGCATTAGATGAATTTGTTGCATCTATTCCTTTTTATGAAACTCGTGGT
TATGTGCAAAATGTAGTCGCTTATGATTTTTACTATCAAATTTTGCAAAATAAAGAAAACCCACAAATCTTTAGCCAAGA
AGAATTGAATCGGCTATACTAA

Upstream 100 bases:

>100_bases
AAAAGACTGGGCTGCACAAGATCCTTATGTAGAAGCTGGCGTATATGCGGATGTTATTGTAAAGCCGTTTAAAAAAGTCT
TTTAATTTAAATAACGTAAT

Downstream 100 bases:

>100_bases
ATGCACTAGTTTAATAGTGTAAAATGGGAGCATAATTATGTATATCAGCCGTAATTTAGAGCAATGGAATGCATTTCTTC
AAATGCTAAAAATTGCCTTT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Peptidoglycan lytic exotransglycosylase

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKKVALISLCIFTALSAFADSPNTATASINLEQEKQNWELAQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNIT
KTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGRQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNA
KENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWQLSEDEIKQWKIAF
LNRFFDNENTDFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTALS
KERGFYPMLAATQLKQAYQLNVPIAPSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKN
QQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKLT
AENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVGKWLSRASGKLALDEFVASIPFYETRG
YVQNVVAYDFYYQILQNKENPQIFSQEELNRLY

Sequences:

>Translated_593_residues
MKKVALISLCIFTALSAFADSPNTATASINLEQEKQNWELAQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNIT
KTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGRQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNA
KENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWQLSEDEIKQWKIAF
LNRFFDNENTDFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTALS
KERGFYPMLAATQLKQAYQLNVPIAPSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKN
QQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKLT
AENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVGKWLSRASGKLALDEFVASIPFYETRG
YVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
>Mature_593_residues
MKKVALISLCIFTALSAFADSPNTATASINLEQEKQNWELAQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNIT
KTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGRQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNA
KENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWQLSEDEIKQWKIAF
LNRFFDNENTDFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTALS
KERGFYPMLAATQLKQAYQLNVPIAPSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKN
QQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKLT
AENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVGKWLSRASGKLALDEFVASIPFYETRG
YVQNVVAYDFYYQILQNKENPQIFSQEELNRLY

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI87082441, Length=383, Percent_Identity=39.9477806788512, Blast_Score=261, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLT_HAEIN (P44888)

Other databases:

- EMBL:   L42023
- PIR:   C64097
- RefSeq:   NP_438989.1
- ProteinModelPortal:   P44888
- SMR:   P44888
- GeneID:   949843
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0829
- NMPDR:   fig|71421.1.peg.797
- TIGR:   HI_0829
- HOGENOM:   HBG495034
- OMA:   PNAYSEY
- ProtClustDB:   CLSK808452
- BioCyc:   HINF71421:HI_0829-MONOMER
- GO:   GO:0005488
- InterPro:   IPR011989
- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189
- Gene3D:   G3DSA:1.25.10.10
- Gene3D:   G3DSA:1.10.1240.20
- Gene3D:   G3DSA:1.25.20.10

Pfam domain/function: PF01464 SLT; SSF48435 Lytic_TGlycosylase_suprhlx_U/L

EC number: 3.2.1.- [C]

Molecular weight: Translated: 68692; Mature: 68692

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALISLCIFTALSAFADSPNTATASINLEQEKQNWELAQHQDYLKRLKQREVFLQVE
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLLKSAVKKQQFSEATQNITKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGRQQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNAKENNRVQKEQNQVENPKQLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWQLSEDEIKQWKIAF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LNRFFDNENTDFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEW
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RYWEAKQDILKNTKKLTALSKERGFYPMLAATQLKQAYQLNVPIAPSFTQAEQLPFKQVF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHH
AMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKNQQWFELAVDASIVAKAWDYL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKLT
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHEEE
AENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVGKWLSRA
ECCCCCCCCCCHHHHHHHHHHEECHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHC
SGKLALDEFVASIPFYETRGYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKKVALISLCIFTALSAFADSPNTATASINLEQEKQNWELAQHQDYLKRLKQREVFLQVE
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLLKSAVKKQQFSEATQNITKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGRQQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNAKENNRVQKEQNQVENPKQLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWQLSEDEIKQWKIAF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LNRFFDNENTDFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEW
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RYWEAKQDILKNTKKLTALSKERGFYPMLAATQLKQAYQLNVPIAPSFTQAEQLPFKQVF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHH
AMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKNQQWFELAVDASIVAKAWDYL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKLT
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHEEE
AENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVGKWLSRA
ECCCCCCCCCCHHHHHHHHHHEECHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHC
SGKLALDEFVASIPFYETRGYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800