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The map label for this gene is rsmD

Identifier: 16272708

GI number: 16272708

Start: 829898

End: 830479

Strand: Reverse

Name: rsmD

Synonym: HI0767

Alternate gene names: 16272708

Gene position: 830479-829898 (Counterclockwise)

Preceding gene: 16272712

Following gene: 16272707

Centisome position: 45.38

GC content: 37.11

Gene sequence:

>582_bases
ATGAAAAAAATACAAACGCCAAATGCAAAGGGCGAGGTTCGCATTATTGCAGGTCTATGGCGAGGGCGAAAACTTCCCGT
ATTAAATTCTGAAGGCTTACGCCCAACTGGCGATAGAGTGAAAGAAACGCTTTTTAATTGGTTAATGCCTTATATCCACC
AATCTGAATGTTTAGATGGCTTTGCGGGGAGTGGTTCATTAGGTTTTGAAGCACTTTCTCGCCAAGCGAAAAAAGTGACT
TTTTTAGAACTAGATAAAACTGTCGCCAATCAATTAAAAAAGAATTTACAAACACTCAAATGCTCATCAGAACAGGCTGA
AGTAATTAATCAAAGTAGTTTGGATTTTCTAAAACAGCCACAAAATCAACCGCACTTTGATGTGGTATTTTTAGATCCTC
CATTTCATTTTAATTTGGCAGAACAAGCAATTAGTTTGCTTTGTGAAAATAACTGGCTAAAACCCAATGCGTTAATTTAC
GTAGAAACAGAGAAAGATAAACCGCTCATCACTCCTGAAAACTGGACGTTATTGAAAGAAAAAACGACGGGAATAGTGAG
CTATCGTTTATATCAGAATTAG

Upstream 100 bases:

>100_bases
CCATAAAATGCTCTCTTAACTGTAATCCGATAAAAATGTCGTTCATTCTAGCAAAAAATCGTCTAAACTAAGCACAATTT
TCAATAAAAATCGAATCTTT

Downstream 100 bases:

>100_bases
AGAGAAAATTATGTTAGATATCGTTTTATATGAACCAGAAATCCCACAAAATACTGGTAACATTATTCGACTTTGTGCCA
ACACAGGATTTCGCTTGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGIVSYRLYQN

Sequences:

>Translated_193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGIVSYRLYQN
>Mature_193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGIVSYRLYQN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family

Homologues:

Organism=Escherichia coli, GI1789875, Length=192, Percent_Identity=54.6875, Blast_Score=204, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMD_HAEIN (P44869)

Other databases:

- EMBL:   L42023
- PIR:   F64158
- RefSeq:   NP_438926.1
- PDB:   2IFT
- PDBsum:   2IFT
- ProteinModelPortal:   P44869
- SMR:   P44869
- GeneID:   949786
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0767
- NMPDR:   fig|71421.1.peg.735
- TIGR:   HI_0767
- HOGENOM:   HBG706795
- OMA:   IRPTADR
- ProtClustDB:   CLSK870517
- BioCyc:   HINF71421:HI_0767-MONOMER
- BRENDA:   2.1.1.52
- InterPro:   IPR002052
- InterPro:   IPR004398
- PIRSF:   PIRSF004553
- TIGRFAMs:   TIGR00095

Pfam domain/function: PF03602 Cons_hypoth95

EC number: =2.1.1.171

Molecular weight: Translated: 22002; Mature: 22002

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAGSGSLGFEALSRQAKKVTFLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQP
CCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIYVETEKDKPLITPENWTLLKE
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCEEEEE
KTTGIVSYRLYQN
CCCCEEEEEEECC
>Mature Secondary Structure
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAGSGSLGFEALSRQAKKVTFLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQP
CCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIYVETEKDKPLITPENWTLLKE
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCEEEEE
KTTGIVSYRLYQN
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800