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The map label for this gene is tagI

Identifier: 16272597

GI number: 16272597

Start: 698164

End: 698721

Strand: Reverse

Name: tagI

Synonym: HI0654

Alternate gene names: 16272597

Gene position: 698721-698164 (Counterclockwise)

Preceding gene: 16272598

Following gene: 16272595

Centisome position: 38.18

GC content: 39.25

Gene sequence:

>558_bases
ATGACTACTCGATGCCCTTGGGTTGGCGAACAATCTATTTATATTGATTATCATGACAAGGAATGGGGAAAGCCTGAATT
CGACAGCCAAAAGCTATTTGAAAAAATTTGTTTGGAAGGGCAGCAAGCGGGGCTTTCGTGGATTACGGTTTTGAAAAAAC
GTGAATCTTACCGTGAGGCTTTTCATCAATTTGATCCGAAAAAAATTGCAAAAATGACCGCACTTGATATTGATGCTTGC
ATGCAAAATTCAGGATTAATTCGCCATCGAGCCAAGTTAGAAGCCATTGTTAAAAATGCGAAAGCTTATTTGGCGATGGA
AAAGTGCGGTGAAAATTTCAGTGATTTTATTTGGTCATTTGTAAATCATAAACCGATCGTCAATGATGTGCCTGATTTGC
GATCTGTGCCAACTAAAACAGAAGTATCGAAAGCACTTTCTAAAGCCTTAAAAAAACGTGGTTTCGTCTTTATTGGCGAA
ACCACGTGCTATGCGTTTATGCAATCTATGGGGCTGGTGGACGATCATTTAAATGATTGTCCTTGCAAAACTAGCTAG

Upstream 100 bases:

>100_bases
TTGGAATGTTAGTCGCACAAGCAGCACATTCTTTCCATTTATGGCGAGGCGTAATGCCAGATTTTGTCTCGGTTTATGAA
CAGCTTAAAAAGGCGATGTT

Downstream 100 bases:

>100_bases
TGTTTATGCTGATCGCGCTCCCATAAATCGGCGTATTTTACAAAGGTTGAATGCGCCGATAACACCGCTAATAAAAATCC
TTGTTTGCCATCTAAAAAAC

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTTRCPWVGEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREAFHQFDPKKIAKMTALDIDAC
MQNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSFVNHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGE
TTCYAFMQSMGLVDDHLNDCPCKTS

Sequences:

>Translated_185_residues
MTTRCPWVGEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREAFHQFDPKKIAKMTALDIDAC
MQNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSFVNHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGE
TTCYAFMQSMGLVDDHLNDCPCKTS
>Mature_184_residues
TTRCPWVGEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREAFHQFDPKKIAKMTALDIDACM
QNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSFVNHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGET
TCYAFMQSMGLVDDHLNDCPCKTS

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=179, Percent_Identity=62.5698324022346, Blast_Score=248, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 3MGA_HAEIN (P44321)

Other databases:

- EMBL:   L42023
- PIR:   G64084
- RefSeq:   NP_438814.1
- PDB:   1V1N
- PDBsum:   1V1N
- ProteinModelPortal:   P44321
- SMR:   P44321
- GeneID:   949931
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0654
- NMPDR:   fig|71421.1.peg.624
- TIGR:   HI_0654
- HOGENOM:   HBG747469
- OMA:   YVAYHDT
- ProtClustDB:   CLSK870542
- BioCyc:   HINF71421:HI_0654-MONOMER
- BRENDA:   3.2.2.20
- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597
- Gene3D:   G3DSA:1.10.340.30
- TIGRFAMs:   TIGR00624

Pfam domain/function: PF03352 Adenine_glyco; SSF48150 DNA_glycsylse

EC number: =3.2.2.20

Molecular weight: Translated: 21194; Mature: 21062

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRCPWVGEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREA
CCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FHQFDPKKIAKMTALDIDACMQNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSF
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VNHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGETTCYAFMQSMGLVDDHLNDC
HCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHCCCCC
PCKTS
CCCCC
>Mature Secondary Structure 
TTRCPWVGEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREA
CCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FHQFDPKKIAKMTALDIDACMQNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSF
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VNHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGETTCYAFMQSMGLVDDHLNDC
HCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHCCCCC
PCKTS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800