Click here to switch to the map view.

The map label for this gene is rep

Identifier: 16272592

GI number: 16272592

Start: 693363

End: 695375

Strand: Reverse

Name: rep

Synonym: HI0649

Alternate gene names: 16272592

Gene position: 695375-693363 (Counterclockwise)

Preceding gene: 16272593

Following gene: 30995393

Centisome position: 38.0

GC content: 40.83

Gene sequence:

>2013_bases
ATGAAACTCAATCCTCAACAACAACAAGCCGTTGAATATGTGACAGGTCCTTGTCTTGTGCTTGCTGGTGCAGGCTCTGG
CAAAACTCGCGTAATCATTAATAAAATCGCCCATTTAATTGAAAAGTGCGGTTATTCTCCGAAACAAATTGCCGCCGTCA
CTTTTACCAATAAAGCCGCACGCGAGATGAAAGAGCGTGTGGCACATTCCATTGGCAAAGAACAATCTAAAGGCTTGCTT
GTTTCCACTTTTCATACGCTCGGTTTTGACATTCTTAAGCGTGAATATAAAGCGTTGGGCTTTAAATCAAATATGACCTT
GTTTGATGAGCACGATCAATTTGCGTTGTTAAAAGAGCTAACCGCTGATGTGTTAAAAGAAGATAAGGATTTATTGCGCG
AGTTAATTTCAGTGATTTCTAACTGGAAGAATGATTTGATTTCGCCAAAACAGGCGTTTGCGTTGGCGCGTGATGCTAAA
TATCAAACTTTCGCAAAATGTTATGAACGTTACGCCACACAAATTCGAACTTACAATGCTCTTGATTTTGATGATTTGAT
TATGCTTCCGACGTTGTTGTTCAAGCAAAATGAAGAAGTGCGGTCAAAATGGCAGGCAAAAATTCGTTATTTGTTGGTGG
ATGAATATCAAGATACCAATACCAGTCAATATGAACTGATTAAACTTTTAGTGGGTGATCGTGCTTGTTTCACTGTGGTG
GGCGATGATGACCAATCTATTTATTCGTGGCGTGGCGCACGACCAGAAAATATGGTGCGTTTACGCGATGATTTCCCTCG
TTTGAACGTGATTAAGCTAGAGCAAAATTACCGTTCAACCCAGCGTATTTTGCATTGTGCCAATATTTTGATTGATAACA
ATGAGCACGTGTTTGATAAGAAACTTTTTTCAACCATTGGTAAAGGGGAAAAATTGCTTGTCATCGAAGCAAAAAATGAA
GAACACGAAGCAGAGCGGATTGTCGCCGAGTTGATCGCCCATCGTTTTAGCCGTAAAACCAAATATAAAGATTATGCGAT
TTTGTATCGAGGCAATCATCAATCCCGATTACTCGAAAAAGTACTGATGCAAAACCGTATTCCTTACAAAATTTCGGGCG
GTACTTCTTTTTTCTCTCGTGCAGAAATCAAAGATATGATGGCGTATTTACGCTTGGTGGTAAATCAAGATGATGACGCA
GCATTCCTGCGTATTGTAAATACACCGAAGCGTGAAATTGGCACCGCAACCTTACAAAAACTTGGCGAGTTGGCTCAAGA
AAAACACATCAGTTTGTTTGAGGCTATTTTTGAGTTTGAGCTTATTCAACGCATCACGCCAAAAGCCTATGATTCATTGC
AAAAATTTGGTCGATGGATTGTAGAACTTAATGATGAAATTCAACGTTCTGAACCAGAACGTGCGGTACGTTCTATGTTA
TCTGCGATTCATTATGAAGAATATTTGTACGAATACGCGACAAGCCCTAAAGCGGCAGAAATGCAAAGTAAGAATGTCGC
CACGCTATTTGACTGGGTTGCGGATATGTTAAAAGGGGATGAAACCAATGAGCCGATGAACCTTAATCAAGTGGTAACCC
GCCTGACATTACGGGATATGTTAGAGCGAGGCGAAGACGATGATGACAGCGATCAAGTTCAACTGATGACATTACACGCA
TCCAAGGGATTGGAATTCCCTTATGTTTATTTGATTGGTATGGAGGAGGGCATTTTGCCTCACCAAACTAGCATTGATGA
AGACAACGTGGAAGAAGAACGCCGCTTGGCTTATGTGGGGATCACAAGAGCGCAAAAAGAACTCACTTTTTCCTTATGTC
GTGAGCGTCGTCAATATGGCGAATTAGTTCGCCCAGAACCAAGCCGATTTTTAGCTGAATTACCTAATGACGATGTGCTA
TGGGAACGCGATAAACCAAAACTTACCACCGAGCAAAAACAAGAAAAAACACAAAACCAACTTGATAGATTGAGGGCGAT
TTTGAAAAGTTAA

Upstream 100 bases:

>100_bases
TTGAAATGGCGCAACAATGGGGATTGCCAATCTTAGATTTGCCTCTCTCATTTTTATTAGACACTGTATTACTGCCCTAT
GCGTGGGCTCAATAATTCTT

Downstream 100 bases:

>100_bases
TAAAAATCGGCAAGAAATTTTCTGCCGATTTTTTATTTTAAAAGTGCGGTCATTTTTCGTTCGATTTCCACCAATTTTTT
ACCGAATTTTCAATTCCAGC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAHLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDILKREYKALGFKSNMTLFDEHDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRTYNALDFDDLIMLPTLLFKQNEEVRSKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGKGEKLLVIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFEAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSEPERAVRSML
SAIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELTFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKS

Sequences:

>Translated_670_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAHLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDILKREYKALGFKSNMTLFDEHDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRTYNALDFDDLIMLPTLLFKQNEEVRSKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGKGEKLLVIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFEAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSEPERAVRSML
SAIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELTFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKS
>Mature_670_residues
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAHLIEKCGYSPKQIAAVTFTNKAAREMKERVAHSIGKEQSKGLL
VSTFHTLGFDILKREYKALGFKSNMTLFDEHDQFALLKELTADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAK
YQTFAKCYERYATQIRTYNALDFDDLIMLPTLLFKQNEEVRSKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDKKLFSTIGKGEKLLVIEAKNE
EHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEKVLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDA
AFLRIVNTPKREIGTATLQKLGELAQEKHISLFEAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSEPERAVRSML
SAIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDMLERGEDDDDSDQVQLMTLHA
SKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVGITRAQKELTFSLCRERRQYGELVRPEPSRFLAELPNDDVL
WERDKPKLTTEQKQEKTQNQLDRLRAILKS

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=67.0149253731343, Blast_Score=938, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=643, Percent_Identity=39.3468118195956, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=733, Percent_Identity=27.1487039563438, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324477, Length=678, Percent_Identity=23.3038348082596, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): REP_HAEIN (P44804)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_438809.1
- ProteinModelPortal:   P44804
- SMR:   P44804
- GeneID:   949695
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0649
- NMPDR:   fig|71421.1.peg.619
- TIGR:   HI_0649
- HOGENOM:   HBG467281
- OMA:   INKIAYL
- ProtClustDB:   CLSK870351
- BioCyc:   HINF71421:HI_0649-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016
- Gene3D:   G3DSA:1.10.10.160
- PANTHER:   PTHR11070
- TIGRFAMs:   TIGR01074

Pfam domain/function: PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 77747; Mature: 77747

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAHLIEKCGYSPKQIAAVTFTNKAA
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHH
REMKERVAHSIGKEQSKGLLVSTFHTLGFDILKREYKALGFKSNMTLFDEHDQFALLKEL
HHHHHHHHHHHCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHH
TADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAKYQTFAKCYERYATQIRTYNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
LDFDDLIMLPTLLFKQNEEVRSKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDK
CCCCCHHHHCCCCCCHHHEEHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
KLFSTIGKGEKLLVIEAKNEEHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTATLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
LGELAQEKHISLFEAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSEPERAVRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHH
SAIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LERGEDDDDSDQVQLMTLHASKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
ITRAQKELTFSLCRERRQYGELVRPEPSRFLAELPNDDVLWERDKPKLTTEQKQEKTQNQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHH
LDRLRAILKS
HHHHHHHHCC
>Mature Secondary Structure
MKLNPQQQQAVEYVTGPCLVLAGAGSGKTRVIINKIAHLIEKCGYSPKQIAAVTFTNKAA
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHH
REMKERVAHSIGKEQSKGLLVSTFHTLGFDILKREYKALGFKSNMTLFDEHDQFALLKEL
HHHHHHHHHHHCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHH
TADVLKEDKDLLRELISVISNWKNDLISPKQAFALARDAKYQTFAKCYERYATQIRTYNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
LDFDDLIMLPTLLFKQNEEVRSKWQAKIRYLLVDEYQDTNTSQYELIKLLVGDRACFTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPENMVRLRDDFPRLNVIKLEQNYRSTQRILHCANILIDNNEHVFDK
CCCCCHHHHCCCCCCHHHEEHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
KLFSTIGKGEKLLVIEAKNEEHEAERIVAELIAHRFSRKTKYKDYAILYRGNHQSRLLEK
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
VLMQNRIPYKISGGTSFFSRAEIKDMMAYLRLVVNQDDDAAFLRIVNTPKREIGTATLQK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHH
LGELAQEKHISLFEAIFEFELIQRITPKAYDSLQKFGRWIVELNDEIQRSEPERAVRSML
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHH
SAIHYEEYLYEYATSPKAAEMQSKNVATLFDWVADMLKGDETNEPMNLNQVVTRLTLRDM
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LERGEDDDDSDQVQLMTLHASKGLEFPYVYLIGMEEGILPHQTSIDEDNVEEERRLAYVG
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
ITRAQKELTFSLCRERRQYGELVRPEPSRFLAELPNDDVLWERDKPKLTTEQKQEKTQNQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHH
LDRLRAILKS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800