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The map label for this gene is purB

Identifier: 16272582

GI number: 16272582

Start: 680324

End: 681694

Strand: Direct

Name: purB

Synonym: HI0639

Alternate gene names: 16272582

Gene position: 680324-681694 (Clockwise)

Preceding gene: 16272581

Following gene: 16272583

Centisome position: 37.17

GC content: 38.22

Gene sequence:

>1371_bases
ATGCAACTCTCTACACTGACCGCACTTTCCCCTCTTGATGGTCGTTATCAAGATAAAGTAACGCCATTACGTGCTATTTT
TAGCGAATTCGGGTTAATGAAATTCCGTGTAGCTGTGGAAGTTCGTTGGTTACAAAAATTAGCTTCAACGGCAGACATTA
CCGAAGTACCGCCATTTTCTACGCAAGCAAATGCTTTTTTAGATGGAATTGTGGCTAATTTTAATGAGGCCGATGCTGCG
CGTATTAAAGAAATTGAACGCACAACTAATCACGATGTGAAAGCCGTTGAGTATTTTCTGAAAGAGAAAATTCAAAACGA
AGTGGAACTAGTGAAGGTTTCTGAATTTATTCACTTCGCTTGCACCTCAGAAGATATTAACAATCTCTCTCACGCATTAA
TGTTAAGCACCGCTCGTGACGAAGTGATTTTACCAGAATGGCAAAAACTGATTGATGAAATTACTCGTCTTGCAGAAGAA
TATAAAACGATTCCATTACTTTCTCGCACACATGGCCAGCCAGCTTCACCAAGCACCGTGGGCAAAGAAATGGCGAATGT
CGTTTACCGCCTAAAACGTCAATTTAAACAACTCCAAAATGCGGAAATCTTAGGTAAAATCAATGGTGCAGTAGGTAACT
ACAATGCGCATTTATCGGCATATCCTAATATTGATTGGCATAAATTCAGCGAAGAATTTGTCACTTCATTAGGTATTCAA
TGGAATCCTTATACCACACAAATTGAGCCGCACGATTACATCACTGAATTTTTTGATGCTGTAGTTCGCTTTAATACCAT
TATCATTGATTTTGATCGTGATTTATGGGGATACATTGCATTAAATCACTTTAAACAACGAACGATTGCAGGCGAAATTG
GTTCTTCTACCATGCCACACAAAGTCAATCCAATTGATTTTGAAAACTCAGAAGGCAATCTAGGATTAGCCAATGCAGTG
ATGACCCATTTAGGTCAAAAATTACCAATTTCTCGCTGGCAGCGTGACTTAACAGATTCAACTGTATTACGTAATTTAGG
CGTAGGATTAGGTTATTGTTTGATCGCTTATGCTTCAACACGCAAAGGCATCAGTAAACTTGAAGTAAATCAACCGCACT
TATTAGAAGAACTCAATCAAAACTGGGAAGTTTTGGCTGAACCAATTCAAACCGTAATGCGCCGCTATGGAATAGAAAAA
CCTTACGAAAAATTAAAAGAATTAACGCGAGGTAAACGAGTGACAGAACAGGCTATGCGTGAATTTATTGATAAGTTGGA
TATTCCACAAGAAGAAAAATTACGTTTACAAAAATTAACACCTGCTACTTATATTGGGGCTGCAGTGGAATTAGTAGAAA
AACTATCATAG

Upstream 100 bases:

>100_bases
GGAACAAAATGGCAAATTTTATTTTTCCGCAGAAAATTACTTGCCACCGCAAAACAGATTTATTCTTCTATTTATTAATA
CAATTCTGGAGCAAACATTT

Downstream 100 bases:

>100_bases
GAATTTGGCTCTTTTTTCAACAATATTCTTTACAAGAACATAGCTTTTCTCTATAATCCTGCGCCTGTGTTAGTCAATCT
AACAAATTTCTGGTTGGTGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEVELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYITEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS

Sequences:

>Translated_456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEVELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYITEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
>Mature_456_residues
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFSTQANAFLDGIVANFNEADAA
RIKEIERTTNHDVKAVEYFLKEKIQNEVELVKVSEFIHFACTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEE
YKTIPLLSRTHGQPASPSTVGKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
WNPYTTQIEPHDYITEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYASTRKGISKLEVNQPHLLEELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVTEQAMREFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=77.1428571428572, Blast_Score=755, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_HAEIN (P44797)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_438799.1
- ProteinModelPortal:   P44797
- SMR:   P44797
- GeneID:   949686
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0639
- NMPDR:   fig|71421.1.peg.609
- TIGR:   HI_0639
- HOGENOM:   HBG290028
- OMA:   KVSEFIH
- ProtClustDB:   PRK09285
- BioCyc:   HINF71421:HI_0639-MONOMER
- BRENDA:   4.3.2.2
- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 51994; Mature: 51994

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 91-91 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQANAFLDGIVANFNEADAARIKEIERTTNHDVKAVEYFLKEKIQNEVELVKVSEFIHFA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEEYKTIPLLSRTHGQPASPSTV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHH
GKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYITEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAST
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNQPHLLEELNQNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVTEQAMR
HCCHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
EFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLSTLTALSPLDGRYQDKVTPLRAIFSEFGLMKFRVAVEVRWLQKLASTADITEVPPFS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TQANAFLDGIVANFNEADAARIKEIERTTNHDVKAVEYFLKEKIQNEVELVKVSEFIHFA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLSTARDEVILPEWQKLIDEITRLAEEYKTIPLLSRTHGQPASPSTV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHH
GKEMANVVYRLKRQFKQLQNAEILGKINGAVGNYNAHLSAYPNIDWHKFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYITEFFDAVVRFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMTHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAST
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNQPHLLEELNQNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVTEQAMR
HCCHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
EFIDKLDIPQEEKLRLQKLTPATYIGAAVELVEKLS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800