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The map label for this gene is dusA

Identifier: 16272577

GI number: 16272577

Start: 673496

End: 674479

Strand: Direct

Name: dusA

Synonym: HI0634

Alternate gene names: 16272577

Gene position: 673496-674479 (Clockwise)

Preceding gene: 30995390

Following gene: 16272581

Centisome position: 36.8

GC content: 40.55

Gene sequence:

>984_bases
ATGCCTCAAAATCTACCGCACTTTTACCGCGGCCGTTTTTCTGTTGCACCAATGTTAGATTGGACAACACGCCATTGTCG
TTATTTTCATCGCCAATTTTCAAAGCACGCATTACTTTACACTGAAATGGTCACAGCACCAGCGATTATCCACGCAAAAT
ACGACCACTTGGATTTTGATTTACAAGAAAATCCCGTTGCGTTACAGCTGGGAGGAAGCGATCCTGAACAACTCAAATAT
TGCGCAAAACTTGCTGAGGAACGAGGCTATCACGAAATTAATCTTAATGTAGGATGCCCTTCTGATCGCGTACAAAATGG
AATGTTTGGTGCTTGCTTAATGGCGAAAGCAGATTTGGTAGCAGACTGTGTAGAACAAATGCAAAGTGCGGTCAAAATTC
CAGTCACTGTGAAAACGCGTATTGGCATTGATGAATTAGACAGCTATGAATTTTTATGTGATTTTATTGAAAAGGTTCAA
GGAAAAGGTTGCCAAGAGTTTATTATCCACGCACGAAAAGCTTGGCTTTCAGGCTTAAGTCCCAAAGAAAATAGAGAAAT
TCCGCCATTAGATTATATTCGGGTGTATCAACTAAAACGTGATTTTCCACATCTAACTATTGCAATTAATGGTGGTATAA
AAACCATTGAAGAAATGAAGCAACATTTGCAATATGTGGACGGCGTGATGGTTGGGCGAGAGGCCTATCAAAATCCAAGT
TTACTTGGGCAAATTGACCAAGCCTTATTTGATCCTAACGCACCAATTGTTACCGCTCATGAAGCGGTAGAGTCAATGCT
TCCTTATATTGAACAACAACTTAGCCAAGGAATACACCTTAATCATATCGTCCGTCATATATTAGGCGCATTCCAAAATT
GCAAAGGTGCTCGTCAATGGCGACGCTATTTAAGCGAAAATGCGTTTAAACAAGGCGCAGGAATTGAAGTGGTTGAAACA
GCCTTAAGCTTTGTAGAAAGCTAA

Upstream 100 bases:

>100_bases
CAAAACCATTTTACCACTTTGCTGCAGGCTGTTTTCTCCAGCAAATGCAAGCTCGCCAAGCCGAAGAATTACGTAGCAAA
ACCGAACAAGAGAAATAAAA

Downstream 100 bases:

>100_bases
TCCTAAAAACAAAATAAGCCGCAAAAGTGCGGCTTATTTTTCAGCTGTTTTTCTTACGCAATACGCATTTGAAAAATCAC
CAATTCAGCTTGGCAACAGA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPQNLPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKY
CAKLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQ
GKGCQEFIIHARKAWLSGLSPKENREIPPLDYIRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
LLGQIDQALFDPNAPIVTAHEAVESMLPYIEQQLSQGIHLNHIVRHILGAFQNCKGARQWRRYLSENAFKQGAGIEVVET
ALSFVES

Sequences:

>Translated_327_residues
MPQNLPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKY
CAKLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQ
GKGCQEFIIHARKAWLSGLSPKENREIPPLDYIRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
LLGQIDQALFDPNAPIVTAHEAVESMLPYIEQQLSQGIHLNHIVRHILGAFQNCKGARQWRRYLSENAFKQGAGIEVVET
ALSFVES
>Mature_326_residues
PQNLPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFDLQENPVALQLGGSDPEQLKYC
AKLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQG
KGCQEFIIHARKAWLSGLSPKENREIPPLDYIRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPSL
LGQIDQALFDPNAPIVTAHEAVESMLPYIEQQLSQGIHLNHIVRHILGAFQNCKGARQWRRYLSENAFKQGAGIEVVETA
LSFVES

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40807366, Length=252, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI145693211, Length=326, Percent_Identity=69.3251533742331, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=27.8301886792453, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=26.8595041322314, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24580595, Length=314, Percent_Identity=25.796178343949, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19920448, Length=314, Percent_Identity=25.796178343949, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_HAEIN (P44794)

Other databases:

- EMBL:   L42023
- PIR:   H64155
- RefSeq:   NP_438794.1
- ProteinModelPortal:   P44794
- SMR:   P44794
- GeneID:   950134
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0634
- NMPDR:   fig|71421.1.peg.604
- TIGR:   HI_0634
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- BioCyc:   HINF71421:HI_0634-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 37186; Mature: 37055

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNLPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFD
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
LQENPVALQLGGSDPEQLKYCAKLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQGKGCQEFIIHARKAWLSGLS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PKENREIPPLDYIRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLGQIDQALFDPNAPIVTAHEAVESMLPYIEQQLSQGIHLNHIVRHILGAFQNCKGARQW
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
RRYLSENAFKQGAGIEVVETALSFVES
HHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PQNLPHFYRGRFSVAPMLDWTTRHCRYFHRQFSKHALLYTEMVTAPAIIHAKYDHLDFD
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
LQENPVALQLGGSDPEQLKYCAKLAEERGYHEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVEQMQSAVKIPVTVKTRIGIDELDSYEFLCDFIEKVQGKGCQEFIIHARKAWLSGLS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PKENREIPPLDYIRVYQLKRDFPHLTIAINGGIKTIEEMKQHLQYVDGVMVGREAYQNPS
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLGQIDQALFDPNAPIVTAHEAVESMLPYIEQQLSQGIHLNHIVRHILGAFQNCKGARQW
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
RRYLSENAFKQGAGIEVVETALSFVES
HHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800