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The map label for this gene is mscL [H]

Identifier: 16272569

GI number: 16272569

Start: 666984

End: 667370

Strand: Direct

Name: mscL [H]

Synonym: HI0626

Alternate gene names: 16272569

Gene position: 666984-667370 (Clockwise)

Preceding gene: 16272568

Following gene: 16272570

Centisome position: 36.44

GC content: 33.33

Gene sequence:

>387_bases
ATGAATTTTATTAAAGAATTTCGCGAATTTGCAATGCGTGGCAATGTCGTAGATATGGCTGTCGGTGTGATTATTGGTAG
CGCATTTGGCAAGATCGTAAGTTCACTTGTTAGTGATATTTTTACGCCCGTGTTGGGGATTTTAACTGGTGGTATTGATT
TCAAAGATATGAAATTTGTATTAGCACAAGCACAAGGCGATGTTCCTGCAGTTACGTTAAATTATGGTTTGTTTATTCAA
AATGTGATTGATTTTATTATTATTGCTTTTGCAATCTTTATGATGATCAAAGTAATCAATAAGGTAAGAAAACCAGAGGA
GAAAAAAACTGCACCAAAGGCAGAAACATTGCTTACTGAAATCCGTGATTTATTAAAGAATAAATAA

Upstream 100 bases:

>100_bases
ATTCCAACCAAGCGCATTCTTTATTTAGTTTTTTTACTTTTCAGAGTGATTTATTATAATAGTCGCTCTTTTTATGTAAT
TTTTGAAGGGGAACATTATT

Downstream 100 bases:

>100_bases
TATTTAAAGAATGAAAGTTAGCAGAAATGCTGACTTTTTTTATTACTATTTTTTAAGCTATAATCAGCCCTCAATTTTAG
GGAAATAAAATGAAAAAATA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNFIKEFREFAMRGNVVDMAVGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK

Sequences:

>Translated_128_residues
MNFIKEFREFAMRGNVVDMAVGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK
>Mature_128_residues
MNFIKEFREFAMRGNVVDMAVGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=132, Percent_Identity=69.6969696969697, Blast_Score=176, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14198; Mature: 14198

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIKEFREFAMRGNVVDMAVGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAQAQGDVPAVTLNYGLFIQNVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
IRDLLKNK
HHHHHCCC
>Mature Secondary Structure
MNFIKEFREFAMRGNVVDMAVGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAQAQGDVPAVTLNYGLFIQNVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
IRDLLKNK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA