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The map label for this gene is hlpA

Identifier: 16272562

GI number: 16272562

Start: 653459

End: 654280

Strand: Reverse

Name: hlpA

Synonym: HI0620

Alternate gene names: 16272562

Gene position: 654280-653459 (Counterclockwise)

Preceding gene: 16272563

Following gene: 16272557

Centisome position: 35.75

GC content: 35.4

Gene sequence:

>822_bases
ATGAAATTAAAACAACTTTTTGCAATCACTGCAATCGCATCAGCTCTCGTTTTAACAGGCTGTAAAGAAGACAAAAAACC
TGAAGCTGCAGCAGCACCGCTTAAAATCAAAGTAGGCGTGATGTCTGGCCCAGAGCATCAAGTTGCAGAAATTGCAGCAA
AAGTCGCTAAAGAAAAATATGGTTTAGACGTTCAATTCGTTGAATTCAATGACTACGCATTACCAAATGAAGCTGTATCT
AAAGGTGATTTAGATGCAAACGCAATGCAACATAAACCTTATTTAGATGAAGATGCAAAAGCGAAAAATTTAAATAACTT
AGTTATCGTGGGTAATACTTTCGTCTATCCATTAGCGGGTTATTCTAAAAAAATCAAAAATGTGAATGAATTACAAGACG
GTGCTAAAGTTGTTGTTCCTAACGATCCAACAAACCGTGGTCGTGCATTAATTCTTCTTGAAAAACAAGGTTTAATCAAA
TTAAAAGATGCAAATAACCTTCTTTCAACTGTATTAGATATTGTTGAAAATCCGAAAAAATTAAACATCACTGAAGTAGA
TACTTCTGTTGCGGCACGCGCATTAGACGACGTTGATTTAGCTGTAGTAAACAATACTTATGCGGGTCAAGTAGGCTTAA
ATGCTCAAGATGACGGTGTATTTGTAGAAGATAAAGATTCTCCATATGTGAACATTATCGTTTCTCGTACCGATAACAAA
GACAGCAAAGCTGTTCAAGATTTCATAAAATCTTACCAAACAGAAGAAGTTTACCAAGAAGCTCAAAAACACTTTAAAGA
TGGCGTTGTAAAAGGTTGGTAA

Upstream 100 bases:

>100_bases
TATTCGTTATGATTAGCCAAAAACTCGGCGATACACTGGCTAAAAAAGTGGATCATCGTTAATTAAACGCAACTCTTAAC
TTAAACAGGAAGGAAATCCT

Downstream 100 bases:

>100_bases
TTTCTACCGCACTTTAACCCATTACTAAATCCCTTGCCCAGCAAGGGATTTTCTTATCTAAGCTCGGAATGTTTTAAAGA
ATATTGGTAGCTAAAAATAA

Product: putative D-methionine-binding lipoprotein MetQ

Products: NA

Alternate protein names: 28 kDa outer membrane protein

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKLKQLFAITAIASALVLTGCKEDKKPEAAAAPLKIKVGVMSGPEHQVAEIAAKVAKEKYGLDVQFVEFNDYALPNEAVS
KGDLDANAMQHKPYLDEDAKAKNLNNLVIVGNTFVYPLAGYSKKIKNVNELQDGAKVVVPNDPTNRGRALILLEKQGLIK
LKDANNLLSTVLDIVENPKKLNITEVDTSVAARALDDVDLAVVNNTYAGQVGLNAQDDGVFVEDKDSPYVNIIVSRTDNK
DSKAVQDFIKSYQTEEVYQEAQKHFKDGVVKGW

Sequences:

>Translated_273_residues
MKLKQLFAITAIASALVLTGCKEDKKPEAAAAPLKIKVGVMSGPEHQVAEIAAKVAKEKYGLDVQFVEFNDYALPNEAVS
KGDLDANAMQHKPYLDEDAKAKNLNNLVIVGNTFVYPLAGYSKKIKNVNELQDGAKVVVPNDPTNRGRALILLEKQGLIK
LKDANNLLSTVLDIVENPKKLNITEVDTSVAARALDDVDLAVVNNTYAGQVGLNAQDDGVFVEDKDSPYVNIIVSRTDNK
DSKAVQDFIKSYQTEEVYQEAQKHFKDGVVKGW
>Mature_273_residues
MKLKQLFAITAIASALVLTGCKEDKKPEAAAAPLKIKVGVMSGPEHQVAEIAAKVAKEKYGLDVQFVEFNDYALPNEAVS
KGDLDANAMQHKPYLDEDAKAKNLNNLVIVGNTFVYPLAGYSKKIKNVNELQDGAKVVVPNDPTNRGRALILLEKQGLIK
LKDANNLLSTVLDIVENPKKLNITEVDTSVAARALDDVDLAVVNNTYAGQVGLNAQDDGVFVEDKDSPYVNIIVSRTDNK
DSKAVQDFIKSYQTEEVYQEAQKHFKDGVVKGW

Specific function: This protein is a component of a D-methionine permease, a binding protein-dependent, ATP-driven transport system

COG id: COG1464

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface antigen

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpA lipoprotein family

Homologues:

Organism=Escherichia coli, GI1786396, Length=275, Percent_Identity=60, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1790093, Length=240, Percent_Identity=54.1666666666667, Blast_Score=266, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METQ_HAEIN (P31728)

Other databases:

- EMBL:   M59804
- EMBL:   L42023
- PIR:   B64082
- RefSeq:   NP_438778.1
- ProteinModelPortal:   P31728
- SMR:   P31728
- GeneID:   950676
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0620
- NMPDR:   fig|71421.1.peg.589
- TIGR:   HI_0620
- HOGENOM:   HBG661773
- OMA:   NIICART
- ProtClustDB:   CLSK892375
- BioCyc:   HINF71421:HI_0620-MONOMER
- InterPro:   IPR004872
- InterPro:   IPR004478
- TIGRFAMs:   TIGR00363

Pfam domain/function: PF03180 Lipoprotein_9

EC number: NA

Molecular weight: Translated: 29828; Mature: 29828

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKQLFAITAIASALVLTGCKEDKKPEAAAAPLKIKVGVMSGPEHQVAEIAAKVAKEKY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
GLDVQFVEFNDYALPNEAVSKGDLDANAMQHKPYLDEDAKAKNLNNLVIVGNTFVYPLAG
CCCEEEEECCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEECCCEEEECCC
YSKKIKNVNELQDGAKVVVPNDPTNRGRALILLEKQGLIKLKDANNLLSTVLDIVENPKK
HHHHHCCHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHCCCCE
LNITEVDTSVAARALDDVDLAVVNNTYAGQVGLNAQDDGVFVEDKDSPYVNIIVSRTDNK
EEEEECHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCEEEECCCCCEEEEEEEECCCC
DSKAVQDFIKSYQTEEVYQEAQKHFKDGVVKGW
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLKQLFAITAIASALVLTGCKEDKKPEAAAAPLKIKVGVMSGPEHQVAEIAAKVAKEKY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
GLDVQFVEFNDYALPNEAVSKGDLDANAMQHKPYLDEDAKAKNLNNLVIVGNTFVYPLAG
CCCEEEEECCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEECCCEEEECCC
YSKKIKNVNELQDGAKVVVPNDPTNRGRALILLEKQGLIKLKDANNLLSTVLDIVENPKK
HHHHHCCHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHCCCCE
LNITEVDTSVAARALDDVDLAVVNNTYAGQVGLNAQDDGVFVEDKDSPYVNIIVSRTDNK
EEEEECHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCEEEECCCCCEEEEEEEECCCC
DSKAVQDFIKSYQTEEVYQEAQKHFKDGVVKGW
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1987077; 7542800; 10675023