Click here to switch to the map view.

The map label for this gene is greB

Identifier: 16272512

GI number: 16272512

Start: 590531

End: 591007

Strand: Direct

Name: greB

Synonym: HI0569

Alternate gene names: 16272512

Gene position: 590531-591007 (Clockwise)

Preceding gene: 16272507

Following gene: 16272515

Centisome position: 32.27

GC content: 41.3

Gene sequence:

>477_bases
ATGGCAAAATCAAATTATATTACCCGTCAAGGCTGGCTTGCGCTTGACCAAGAACTCAAGTTTTTGTGGAAAGAAGAACG
CCCAAAAGTCACTCAAGCCGTTTCAGATGCAGCTGCGCTAGGCGATCGTAGCGAAAATGCCGAATATATTTATGGTAAAC
GTCGTTTACGTGAAATTGATCGCCGCGTGCGTTTTTTAACTAAAAGATTAGAAGTGCTACAAATTGTCGATTACAACCCA
AAGCAAGAGGGAAAAGTTTTCTTCGGTGCGTGGGTGGAATTAGAAAATGAAGAAGGCGAAACCAAGCAATATCGTATCGT
CGGTTGTGATGAATTTGCCCCTGCGAAAAATTGGATATCCATTGATTCCCCAGTTGCTCGAGCTTTAATCGGAAAAACAT
TAGACGACGAAGTGCGGGTAGAAACGCCCTCAGGTTTTATCACACTTTATGTGAATAAAATTTGGTATGAAAAATAA

Upstream 100 bases:

>100_bases
AACATTGTCGTACTTCTTTATTATAAAAAAACGAAGATAGAATACTGGTTTTAGCGGGATGGCTCAAATATAATTCAAAA
AACTTTCTTTTATTTTTATT

Downstream 100 bases:

>100_bases
CTTGGAAGAATAAAAGATAACAAATACCTAAGCACAAAGAAAAACTAATTGACTAGATATAAAAACTATCTTACTTGCCT
CTTTCCTTATTCGCTGCAAA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNP
KQEGKVFFGAWVELENEEGETKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK

Sequences:

>Translated_158_residues
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNP
KQEGKVFFGAWVELENEEGETKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
>Mature_157_residues
AKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYNPK
QEGKVFFGAWVELENEEGETKQYRIVGCDEFAPAKNWISIDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=61.5384615384615, Blast_Score=190, Evalue=3e-50,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=37.5, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_HAEIN (P43882)

Other databases:

- EMBL:   L42023
- PIR:   B64078
- RefSeq:   NP_438726.1
- ProteinModelPortal:   P43882
- SMR:   P43882
- GeneID:   950647
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0569
- NMPDR:   fig|71421.1.peg.539
- TIGR:   HI_0569
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- BioCyc:   HINF71421:HI_0569-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18464; Mature: 18333

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYNPKQEGKVFFGAWVELENEEGETKQYRIVGCDEFAPAKNWIS
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCEE
IDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEECCC
>Mature Secondary Structure 
AKSNYITRQGWLALDQELKFLWKEERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYNPKQEGKVFFGAWVELENEEGETKQYRIVGCDEFAPAKNWIS
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCEE
IDSPVARALIGKTLDDEVRVETPSGFITLYVNKIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800