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The map label for this gene is gyrB

Identifier: 16272510

GI number: 16272510

Start: 585576

End: 587996

Strand: Reverse

Name: gyrB

Synonym: HI0567

Alternate gene names: 16272510

Gene position: 587996-585576 (Counterclockwise)

Preceding gene: 16272511

Following gene: 30995386

Centisome position: 32.13

GC content: 41.06

Gene sequence:

>2421_bases
ATGTCAGAAACGACCAATGATAACTATGGTGCATCGAGCATTAAAGTATTAAAAGGCCTTGATGCGGTGCGTAAGCGTCC
TGGTATGTATATCGGCGATACCGATGACGGTACTGGTTTGCACCATATGGTATTTGAAGTGGTGGATAATGCCATTGATG
AAGCCCTCGCTGGCCATTGTTCCGATATTATCGTGACAATTCACGATGATAATTCTGTATCGGTGCAAGATGATGGGGGC
GGGATTCCTGTGGATATTCATCCTGAAGAAGGCGTTTCTGCAGCAGAAGTTATTATGACTGTGCTTCACGCAGGCGGTAA
ATTTGACGATAACTCTTATAAAGTATCGGGCGGTTTACACGGCGTGGGCGTATCTGTAGTGAATGCGCTTTCTGATAAGT
TACAATTAACCATTCGTCGTCAAGGTCACGTTCACGAGCAGTTTTATCATTTAGGGGAGCCTCAATCGCCATTAACAGTG
ATTGGCGAAACAGAGGCAACGGGGACAACGGTTCGTTTCTGGCCAAGCTCAGATATTTTTGCTATCACAACCTTTGATTA
CAAAATCTTAGCAAAACGCTTACGTGAGCTTTCTTTCTTGAATTCTGGCGTATCTATTCGTTTAATCGATAAACGTGATG
GATCTGAAGATCATTTCCATTATGAAGGCGGTATTCAAGCATTCGTAGAATATTTGAACAAAAATAAAAACCCAATTCAT
CCAAAGCCATTTTATTTTACCGCTGAGAAAGATGGCATCGGCGTAGAAGTCGCCTTGCAATGGAATGATGGTGTAAATGA
AAACGTGTACTGCTTTACCAATAACATTCCTCAACGTGATGGCGGTACTCACTTAGCTGGTTTTCGTGGAGCTTTAACCC
GTAGCTTAAATAGCTATATGGAAAACGAAGGCATGCTGAAAAAAGAAAAAGTGGCGACTTCAGGCGATGATGCACGTGAA
GGTTTGGTGGCGATTATTTCCGTTAAAGTGCCTGATCCAAAATTCTCATCGCAAACGAAAGACAAACTCGTGTCTTCAGA
AGTTAAAAGCGCGGTGGAATCTGCGATGAATGAAAAAATGCAGGAATATTTATTGGAAAATCCTGCAGATGCAAAAATCA
TTGTAAATCAAATTATTATGGCAGCGCGTGCGCGTGAAGCAGCACGTAAAGCGCGTGAAATGACGCGCCGTAAAGGGGCA
TTAGATATTGCAGGTTTACCAGGTAAACTAGCAGACTGCCAAGAAAAAGATCCTGCTCTTTCTGAACTTTACTTGGTGGA
GGGGGACTCCGCGGGTGGTTCTGCAAAAGTTGGTCGTGATCGTAAAACTCAAGCAATTTTGCCATTGAAAGGTAAAATCT
TAAACGTAGAAAAAGCACGTTTCGACAAAATGTTGTCTTCCCAAGAAGTGGGCACATTAATTACTGCTTTAGGCTGCGGT
ATTGGTCGTGATGAATATAATCCAGATAAATTACGTTATCATCATATCATTATTATGACCGATGCTGATGTGGATGGTTC
TCACATCCGTACGTTGCTTTTAACCTTCTTCTATCGTCAAATGCCTGAGCTGATTGAGCGTGGTTATGTTTACATTGCTC
AGCCGCCACTTTACAAAGTGAAAAAAGGAAAACAAGAACGCTACATTAAAGATGCGGATGAAATGGAGCAATATGAATTA
ACCCTTGCGTTAGATGGCGCAGAATTACATATCAGCACAAATGCACCAGCAATGAATGCCCTTGTATTTGAGAAATTGGT
GGCAGAATATAACAGCGTACAAAAACTTATCGGTCGTTTAAACCGTCATTACCCAGCCCCTGTATTGCAAGGATTAATTT
ATCAGTCGCCAATTTCTATTGAAATGATGAAAGAGGAAAGTGCGGTGGAAAATTGGGGTAAATCTTTTGTTGAACAACTG
ACTGCAAAAGAAACTGAAGCACATCAATATTCAGTGCGTACACAATTTAATGCGGAACGCCAAGTGTATGAAGCGGTGAT
TACTGTTCGCAAACACGGTATTGATACCGATTATTTCTTGAATTTTGATTTTGTTCACGGCAATGAATACGCAAAAATTG
TTTCTCTTAATAAACAACTCAATGGTTTATTAGAAGAGGGTGCTTATGTTATTCGTGGCGAAAAAGTACAACCAGTGCGT
AGTTTTGAACAAGCCGTTGAATGGTTAGTGAAAGAATCTCGTAAAGGTCTTGAAGTACAACGTTATAAAGGGCTTGGGGA
AATGAATGCAGATCAACTTTGGGAAACCACAATGGATCCAAATTCACGTCGTATGTTGAAAGTTTCTATTAAAGATGCTG
TTGCAGCAGATCAATTATTCACTACCTTAATGGGAGATGAAGTTGAGCCACGTCGTGAGTTCATTGAGCTGAATGCGTTA
AGAGCAAATTTGGACGTGTAA

Upstream 100 bases:

>100_bases
CCTTTTTTTATCGTTTTCCTTTCCCGAAAAGCATCGCCAAAACGGCGATTTTTTGCTATAATCTCGCCCAATTTTTATTT
ACAAAAGAATGAGATAAATT

Downstream 100 bases:

>100_bases
ATTTTCGTCTATTTATATTCATGACTGAAAGGGCACCAAAGAGCCCTTTCTTTGTTTATCTTGTTATTTCATATAAGGAT
TACTGTTGGTTTTTTGACGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGG
GIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQSPLTV
IGETEATGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGSEDHFHYEGGIQAFVEYLNKNKNPIH
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDARE
GLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGA
LDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYEL
TLALDGAELHISTNAPAMNALVFEKLVAEYNSVQKLIGRLNRHYPAPVLQGLIYQSPISIEMMKEESAVENWGKSFVEQL
TAKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
SFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNAL
RANLDV

Sequences:

>Translated_806_residues
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGG
GIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQSPLTV
IGETEATGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGSEDHFHYEGGIQAFVEYLNKNKNPIH
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDARE
GLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGA
LDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYEL
TLALDGAELHISTNAPAMNALVFEKLVAEYNSVQKLIGRLNRHYPAPVLQGLIYQSPISIEMMKEESAVENWGKSFVEQL
TAKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
SFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNAL
RANLDV
>Mature_805_residues
SETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGGG
IPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQSPLTVI
GETEATGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGSEDHFHYEGGIQAFVEYLNKNKNPIHP
KPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDAREG
LVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGAL
DIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCGI
GRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYELT
LALDGAELHISTNAPAMNALVFEKLVAEYNSVQKLIGRLNRHYPAPVLQGLIYQSPISIEMMKEESAVENWGKSFVEQLT
AKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVRS
FEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNALR
ANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=604, Percent_Identity=25.1655629139073, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI19913406, Length=566, Percent_Identity=25.4416961130742, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=74.9688667496887, Blast_Score=1243, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=555, Percent_Identity=41.4414414414414, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=25.974025974026, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=514, Percent_Identity=24.124513618677, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=34.8387096774194, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=571, Percent_Identity=25.3940455341506, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17136538, Length=543, Percent_Identity=25.9668508287293, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_HAEIN (P43701)

Other databases:

- EMBL:   L42023
- PIR:   A64078
- RefSeq:   NP_438724.1
- ProteinModelPortal:   P43701
- GeneID:   949613
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0567
- NMPDR:   fig|71421.1.peg.537
- TIGR:   HI_0567
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK14939
- BioCyc:   HINF71421:HI_0567-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 89886; Mature: 89755

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SDIIVTIHDDNSVSVQDDGGGIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLH
CEEEEEEECCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQSPLTVIGETEATGTTVRFWPSSDIF
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCEE
AITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGSEDHFHYEGGIQAFVEYLNKNKNPIH
EEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHCCCCCCC
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYM
CCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
ENEGMLKKEKVATSGDDAREGLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKM
HCCCCCCHHHCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
SELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
EEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKV
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KKGKQERYIKDADEMEQYELTLALDGAELHISTNAPAMNALVFEKLVAEYNSVQKLIGRL
HCCCHHHHHCCHHHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRHYPAPVLQGLIYQSPISIEMMKEESAVENWGKSFVEQLTAKETEAHQYSVRTQFNAER
HCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHH
QVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
HHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHCCEEEEECCCCCHHH
SFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLF
HHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHH
TTLMGDEVEPRREFIELNALRANLDV
HHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SDIIVTIHDDNSVSVQDDGGGIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLH
CEEEEEEECCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQSPLTVIGETEATGTTVRFWPSSDIF
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCEE
AITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGSEDHFHYEGGIQAFVEYLNKNKNPIH
EEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHCCCCCCC
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYM
CCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
ENEGMLKKEKVATSGDDAREGLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKM
HCCCCCCHHHCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
SELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
EEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKV
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KKGKQERYIKDADEMEQYELTLALDGAELHISTNAPAMNALVFEKLVAEYNSVQKLIGRL
HCCCHHHHHCCHHHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRHYPAPVLQGLIYQSPISIEMMKEESAVENWGKSFVEQLTAKETEAHQYSVRTQFNAER
HCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHH
QVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
HHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHCCEEEEECCCCCHHH
SFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLF
HHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHH
TTLMGDEVEPRREFIELNALRANLDV
HHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800