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The map label for this gene is hindIIM

Identifier: 16272457

GI number: 16272457

Start: 528932

End: 530488

Strand: Reverse

Name: hindIIM

Synonym: HI0513

Alternate gene names: 16272457

Gene position: 530488-528932 (Counterclockwise)

Preceding gene: 16272458

Following gene: 16272456

Centisome position: 28.99

GC content: 26.14

Gene sequence:

>1557_bases
ATGGATGAATCAAAGAAAATATCACTAGGTCAGTTTTTTACTCCTACACATATTGTTAAATATATGATTGGACTAATGAC
GAAAAATAAAAATGCTAGTATTTTAGAACCTAGTAGTGGGAATGGTGTATTTCTAGATAGTTTAATTCAATTAGGTTATA
CAAATTTAACATCGTATGAAATAGATGGTGATATTATTTCTCATCCATTTGTAATTAACTCAAGTTTTATAACATCATAT
GATAAGCCTCAATATGATTCGATCATTGGAAATCCTCCTTATGTTCGTTGGAAAAACCTAAGTGAGCTTCAGAAGAAAGA
GCTTAAAGATAATTCTATATGGAAAATGTATTGTAATAGTCTTTGTGATTATTTCTATATTTTTATTATTAAATCTATAC
TTCAATTAAAAGTTGGAGGAGAATTAATATTTATTTGCCCTGATTATTTTTTTTCCACTAAGAACGCTGAGGGTTTAAGG
AAATTTTTGATAAATAATGGAAGTTTTGAAAAAATTATATTATTTAATGAAAGTAAAGTATTTCATGGAGTTTCTTCTTC
AGTTGTTATTTTTAAATATATAAAAGGAAAAAATATTGATAATATAAATATTATTAATATAGATTCAAAAAGCCCAATAA
AATCTGAGGATATAGAATCTTTAGGTGAATCATATTATATTCCAAGATTTTCTAGCTCAGATGTTTGGGTTACTTCGCCC
AATCATATTAAAGTTGCATTAGATAAATTTGAGAGTTACTGCAAAACTATCAAAAAAGTTCAGCCAAAATCATTATTTGA
TGATCTTGAGTTTTCTAGAATCGGAAGTGTCTGTGATATTGGGAATGGGATGGTAAGTGGTCTAGATAAGGCTTTTCAGA
TGAATGATATAAATTATTCAGAATTAGAATTGCTTAATTCAATTTGTGTAGCTAAAGCTAAACACTTAGACGCATTTTGT
TTCTCTGGATACACCAGATACAAGTTCATATTAGATGATATTAATGAGGATAAATTAATCACTTATTTTCCAAATTTCTT
TTATGAGTTTAATAATTATAAAGATTATCTATTAAAAAGATATAGTTATAATAAATATTTGCCTTATTGGAAGTGGGCTT
TTCTTAGAAATTTTTCTCTATTCAGCAAGAATGAGAAGAAAATATTTGTGCCGTGCAAAGAAAGAATTTCAAAAAAAAGT
AATTTTAGATTTAGCTTAGTTGATGAATTTATATATCCAACACAGGATGTTACTGCATTATATAAGAAAGAAAATGTAAA
GGAGAGTATTGAGTATATTACAGCATATTTAAACTCTAAAGCTGTGTTTTTATGGATGAAGTATAAGGGTGTTGTCAAAG
GTAATGTAGTTGAATTTTCAGAAAAACCATTAGCAAATATTCCTTTTAGAAGAATAGATTGGCAATTAAAAAGCGAAAAA
AAAATTCATGATGATATAACTAATTTAGTAAGAAAATATTTATCAAACAAAGAATTTTCAATTTTACATGAGATTAACTT
AAATTTAGAAAAATTAGGTATAAAGGTGGAAATATGA

Upstream 100 bases:

>100_bases
ATTGTTAAATTCTGAAATAGAAGATTAATATTTGTATCTTATGTTTAAACCCTCAGAATGAGAGTTCTGAGGGRTTTTAT
TAGAAAAATAGGATAAAAAT

Downstream 100 bases:

>100_bases
GTTTCATAAAACCTATTTATCAGGATATTAATTCAATATTAATCGGGCAAAAAGTGAAACGTCCTAAATCAGGTACTCTG
TCAGGTCATGCTGCAGGGGA

Product: modification methylase

Products: NA

Alternate protein names: M.HindII; Adenine-specific methyltransferase HindII

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFLDSLIQLGYTNLTSYEIDGDIISHPFVINSSFITSY
DKPQYDSIIGNPPYVRWKNLSELQKKELKDNSIWKMYCNSLCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLR
KFLINNGSFEKIILFNESKVFHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLGESYYIPRFSSSDVWVTSP
NHIKVALDKFESYCKTIKKVQPKSLFDDLEFSRIGSVCDIGNGMVSGLDKAFQMNDINYSELELLNSICVAKAKHLDAFC
FSGYTRYKFILDDINEDKLITYFPNFFYEFNNYKDYLLKRYSYNKYLPYWKWAFLRNFSLFSKNEKKIFVPCKERISKKS
NFRFSLVDEFIYPTQDVTALYKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLANIPFRRIDWQLKSEK
KIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI

Sequences:

>Translated_518_residues
MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFLDSLIQLGYTNLTSYEIDGDIISHPFVINSSFITSY
DKPQYDSIIGNPPYVRWKNLSELQKKELKDNSIWKMYCNSLCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLR
KFLINNGSFEKIILFNESKVFHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLGESYYIPRFSSSDVWVTSP
NHIKVALDKFESYCKTIKKVQPKSLFDDLEFSRIGSVCDIGNGMVSGLDKAFQMNDINYSELELLNSICVAKAKHLDAFC
FSGYTRYKFILDDINEDKLITYFPNFFYEFNNYKDYLLKRYSYNKYLPYWKWAFLRNFSLFSKNEKKIFVPCKERISKKS
NFRFSLVDEFIYPTQDVTALYKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLANIPFRRIDWQLKSEK
KIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI
>Mature_518_residues
MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFLDSLIQLGYTNLTSYEIDGDIISHPFVINSSFITSY
DKPQYDSIIGNPPYVRWKNLSELQKKELKDNSIWKMYCNSLCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLR
KFLINNGSFEKIILFNESKVFHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLGESYYIPRFSSSDVWVTSP
NHIKVALDKFESYCKTIKKVQPKSLFDDLEFSRIGSVCDIGNGMVSGLDKAFQMNDINYSELELLNSICVAKAKHLDAFC
FSGYTRYKFILDDINEDKLITYFPNFFYEFNNYKDYLLKRYSYNKYLPYWKWAFLRNFSLFSKNEKKIFVPCKERISKKS
NFRFSLVDEFIYPTQDVTALYKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLANIPFRRIDWQLKSEK
KIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI

Specific function: This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HindII endonuclease

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTHD_HAEIN (P44414)

Other databases:

- EMBL:   L42023
- PIR:   F64073
- RefSeq:   NP_438671.1
- ProteinModelPortal:   P44414
- SMR:   P44414
- REBASE:   3427
- GeneID:   950799
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0513
- NMPDR:   fig|71421.1.peg.484
- TIGR:   HI_0513
- OMA:   PCKERIS
- ProtClustDB:   CLSK2518360
- BioCyc:   HINF71421:HI_0513-MONOMER
- BRENDA:   2.1.1.72
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011639
- PRINTS:   PR00507

Pfam domain/function: PF07669 Eco57I; PF02384 N6_Mtase

EC number: =2.1.1.72

Molecular weight: Translated: 60410; Mature: 60410

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFLDSLIQLGYTNLTSYE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHCCCCCCEEEE
IDGDIISHPFVINSSFITSYDKPQYDSIIGNPPYVRWKNLSELQKKELKDNSIWKMYCNS
ECCCEECCCEEEECHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHH
LCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLRKFLINNGSFEKIILFNESKV
HHHHHHHHHHHHHHHEECCCEEEEECCCHHCCCCCHHHHHHHHHCCCCEEEEEEEECCCE
FHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLGESYYIPRFSSSDVWVTSP
ECCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCEECCCCCCCCEEEECC
NHIKVALDKFESYCKTIKKVQPKSLFDDLEFSRIGSVCDIGNGMVSGLDKAFQMNDINYS
CEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCHHHCCCHHHHHHHHHHEECCCCHH
ELELLNSICVAKAKHLDAFCFSGYTRYKFILDDINEDKLITYFPNFFYEFNNYKDYLLKR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHEECCCHHHHHHHH
YSYNKYLPYWKWAFLRNFSLFSKNEKKIFVPCKERISKKSNFRFSLVDEFIYPTQDVTAL
HCCCCCCCHHHHHHHHCCHHHCCCCCEEEECCHHHCCCCCCCEEEEHHHHHCCCHHHHHH
YKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLANIPFRRIDWQLKSEK
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEECCEEEECCCCCCCCCHHHCCCEECCCC
KIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCEEEEC
>Mature Secondary Structure
MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFLDSLIQLGYTNLTSYE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHCCCCCCEEEE
IDGDIISHPFVINSSFITSYDKPQYDSIIGNPPYVRWKNLSELQKKELKDNSIWKMYCNS
ECCCEECCCEEEECHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHH
LCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLRKFLINNGSFEKIILFNESKV
HHHHHHHHHHHHHHHEECCCEEEEECCCHHCCCCCHHHHHHHHHCCCCEEEEEEEECCCE
FHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLGESYYIPRFSSSDVWVTSP
ECCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCEECCCCCCCCEEEECC
NHIKVALDKFESYCKTIKKVQPKSLFDDLEFSRIGSVCDIGNGMVSGLDKAFQMNDINYS
CEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCHHHCCCHHHHHHHHHHEECCCCHH
ELELLNSICVAKAKHLDAFCFSGYTRYKFILDDINEDKLITYFPNFFYEFNNYKDYLLKR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHEECCCHHHHHHHH
YSYNKYLPYWKWAFLRNFSLFSKNEKKIFVPCKERISKKSNFRFSLVDEFIYPTQDVTAL
HCCCCCCCHHHHHHHHCCHHHCCCCCEEEECCHHHCCCCCCCEEEEHHHHHCCCHHHHHH
YKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLANIPFRRIDWQLKSEK
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEECCEEEECCCCCCCCCHHHCCCEECCCC
KIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800