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The map label for this gene is hisH [H]

Identifier: 16272420

GI number: 16272420

Start: 495356

End: 495955

Strand: Direct

Name: hisH [H]

Synonym: HI0472

Alternate gene names: 16272420

Gene position: 495356-495955 (Clockwise)

Preceding gene: 16272419

Following gene: 16272421

Centisome position: 27.07

GC content: 38.17

Gene sequence:

>600_bases
ATGATAAACATCACGATTATAGACACAGGTTGTGCCAACCTTTCTTCTGTAAAATTCGCCTTTGACCGATTAGGTTACAA
TACCGAGATCACTTTTGATCTCAATAAAATCAAATCTGCCGATAAGCTGATTTTACCTGGCGTAGGCACTGCAAATGCAG
CAATGTATAATTTACAAGAACGTCAATTGATCGAGACCATTCAAAATCTAACACAACCAGTGCTAGGAATTTGTTTGGGT
ATGCAGTTAATGACCGAATTTTCCGAAGAGGGTAATGTGCCGACCTTAAACCTAATAAGCGGAAAAACTAATCGAATTCC
CGATACGGGTTTACCGTTACCTCAAATGGGATGGAATCGCGTGCAATTTGTAAAAAACTGTCCGTTATTTGATGGTATTG
TGCAAAATAGTCACTTCTATTTTGTGCATAGTTATGCAGTTAGCCCGAATGAACATTCGGTGGCAATAAGCAATTATGGT
GTGAATTTTTCTGCTGCGATAGCGAAAGAGAATTTTTACGGTGTACAATTTCATCCTGAACGTTCGGGTAAAAATGGTGC
GTTGTTGCTAAAAAATTTTGTGGAAAAAGTACCGTTTTAA

Upstream 100 bases:

>100_bases
GAAATGAAATGCCAAGTTCAAAAGGAGTTTTATAGTTGAAAGAGCGGTTAATTTTTGTCGTATTTTTGCAAAATTCGACC
GATTGTAAAAGGATAAAAAA

Downstream 100 bases:

>100_bases
TCACTAGATCATATAGTCATACAGGATCAAACACAATGAAACAATCTATTATTATTCCTGCTCTTGATCTTATCAATGGT
CAAGTTGTGCGGTTACACCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MINITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLG
MQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYG
VNFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF

Sequences:

>Translated_199_residues
MINITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLG
MQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYG
VNFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF
>Mature_199_residues
MINITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLG
MQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYG
VNFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=55.8974358974359, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=34.5971563981043, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22049; Mature: 22049

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQE
CEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEECCCCCCCHHHHHHHH
RQLIETIQNLTQPVLGICLGMQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
VQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYGVNFSAAIAKENFYGVQFHPE
HHHHHCCCCHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEECCCEEEEEEECC
RSGKNGALLLKNFVEKVPF
CCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MINITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQE
CEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEECCCCCCCHHHHHHHH
RQLIETIQNLTQPVLGICLGMQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
VQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYGVNFSAAIAKENFYGVQFHPE
HHHHHCCCCHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEECCCEEEEEEECC
RSGKNGALLLKNFVEKVPF
CCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA