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The map label for this gene is mazG

Identifier: 16272408

GI number: 16272408

Start: 480783

End: 481574

Strand: Direct

Name: mazG

Synonym: HI0460

Alternate gene names: 16272408

Gene position: 480783-481574 (Clockwise)

Preceding gene: 16272393

Following gene: 16272410

Centisome position: 26.27

GC content: 38.26

Gene sequence:

>792_bases
ATGCGCTACTCCATTCAAGATTTTATTCAACTCATCGCCCAACTTCGCAATCCAAATGGCGGATGCCCTTGGGATTTAAA
ACAAAATTATGAATCTATGATTTCTTGTTTAACGGAAGAAACTTACGAAGTAATTGAAGCCATTGAGAAAAAAGACATAC
CAAATTTACGCGAAGAATTAGGGGATTTATTGTTGCAAGTCGTTTTCTTCAGTCAGCTTGCTAGGGAAGATAAATACTTT
GCTTTTGACGATGTATTGCAAGATGTTGCTGAAAAAATTGTACGCCGTCATCCTCATGTGTTTGGTGATGCAAAAGCGGG
GGACGAAACAGAAGCTCTTTCCCGTTGGAATGAAATGAAAGCGAAAGAAAAACAAGGTAAAAGTGAAGAAACCTCTATTT
TAGATAATGTGCCTCGTGCTTTGCCTTCTCTTACGCGAGCGGCAAAATTACAAAAACGTTGTTCAAAAGTAGGGTTTGAT
TGGGAAGAAATTTCACCCGTATTTGACAAAGTGCGGGAAGAATTAGAAGAAGTTCAAGCTGAAATTAACCGCACTTCGAT
TGAACAAAATAAAGTGGAAGAGGAAATCGGCGATTTATTGTTCGCAACCGTCAATCTTGCTCGCCACTTAAAATGTGATC
CTGAAGATGCATTGCGGAAAGCAAATTTAAAATTTGAACGTCGTTTTCGAGCAGTAGAGCAAGCGGTTCAACAACAAGGT
AAGCAAGTGAATAATGTGCCACTTATTGAATTAGATTTGTTATGGGATGAAGTGAAAAAACAAGAAAACTAA

Upstream 100 bases:

>100_bases
CACAAAGTATAAAGAAAATACGCCTAAAAAGCGACGGCATTTGCACCATTTTTCTTGTTCGTTATACTATCCAAAACATT
CCATCAAAGGAAACCAAATT

Downstream 100 bases:

>100_bases
TTGAAAAGTGCGGTAAAAATTTCTCTCATTTTTTACCGCACTTTCTTCGTTAAAGTTGCTGAATATCCAGTGCCATTTGG
TCTAACATTTCATAACGTTT

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMISCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLAREDKYF
AFDDVLQDVAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN

Sequences:

>Translated_263_residues
MRYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMISCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLAREDKYF
AFDDVLQDVAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN
>Mature_263_residues
MRYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMISCLTEETYEVIEAIEKKDIPNLREELGDLLLQVVFFSQLAREDKYF
AFDDVLQDVAEKIVRRHPHVFGDAKAGDETEALSRWNEMKAKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFD
WEEISPVFDKVREELEEVQAEINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
KQVNNVPLIELDLLWDEVKKQEN

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family

Homologues:

Organism=Escherichia coli, GI1789144, Length=258, Percent_Identity=49.2248062015504, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAZG_HAEIN (P44723)

Other databases:

- EMBL:   L42023
- PIR:   H64069
- RefSeq:   NP_438621.1
- ProteinModelPortal:   P44723
- SMR:   P44723
- GeneID:   949607
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0460
- NMPDR:   fig|71421.1.peg.434
- TIGR:   HI_0460
- HOGENOM:   HBG616899
- OMA:   LRAYRVQ
- ProtClustDB:   PRK09562
- BioCyc:   HINF71421:HI_0460-MONOMER
- InterPro:   IPR004518
- InterPro:   IPR011551
- TIGRFAMs:   TIGR00444

Pfam domain/function: PF03819 MazG

EC number: =3.6.1.8

Molecular weight: Translated: 30520; Mature: 30520

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: BINDING 177-177 BINDING 198-198 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMISCLTEETYEVIEAIEKKDIPNLREEL
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDLLLQVVFFSQLAREDKYFAFDDVLQDVAEKIVRRHPHVFGDAKAGDETEALSRWNEMK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFDWEEISPVFDKVREELEEVQA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHC
KQVNNVPLIELDLLWDEVKKQEN
CCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYSIQDFIQLIAQLRNPNGGCPWDLKQNYESMISCLTEETYEVIEAIEKKDIPNLREEL
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDLLLQVVFFSQLAREDKYFAFDDVLQDVAEKIVRRHPHVFGDAKAGDETEALSRWNEMK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AKEKQGKSEETSILDNVPRALPSLTRAAKLQKRCSKVGFDWEEISPVFDKVREELEEVQA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EINRTSIEQNKVEEEIGDLLFATVNLARHLKCDPEDALRKANLKFERRFRAVEQAVQQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHC
KQVNNVPLIELDLLWDEVKKQEN
CCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800