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The map label for this gene is topB

Identifier: 16272392

GI number: 16272392

Start: 465334

End: 467289

Strand: Direct

Name: topB

Synonym: HI0444

Alternate gene names: 16272392

Gene position: 465334-467289 (Clockwise)

Preceding gene: 16272391

Following gene: 16272393

Centisome position: 25.43

GC content: 40.29

Gene sequence:

>1956_bases
ATGCGTTTATTTATCGCCGAAAAACCAAGCTTAGCTCGCGCTATTGCTGATGTGCTCCCGAAACCCCATCAACGTGGGGA
TGGTTTTATTAAATGCGGCGATAATGATGTGGTAACTTGGTGTGTAGGGCATTTGCTCGAGCAAGCAGAACCCGATGCCT
ATGATCCTAAATTCAAACAATGGCGTTTAGAACATCTCCCTATCATTCCTGAAAAATGGCAACTTTTACCGCGAAAAGAA
GTAAAAAAACAACTTTCTGTGGTAGAAAAACTGATCCATCAAGCGGATACACTTGTTAATGCAGGAGACCCTGATAGAGA
AGGACAATTACTTGTAGATGAAGTGTTCAGTTATGCCAATTTATCTGCCGAAAAACGCGATAAAATTTTACGCTGTTTGA
TTAGCGACCTAAATCCAAGTGCGGTAGAAAAAGCGGTTAAAAAATTACAACCCAACCGTAATTTTATTCCTCTTGCCACA
TCCGCTCTCGCACGAGCTCGCGCCGATTGGCTTTATGGCATTAATATGACCAGAGCTTATACCATTCGAGGTAGACAAAC
TGGCTATGATGGCGTGCTTTCTGTTGGACGAGTGCAAACCCCTGTGCTAGGTTTAATTGTACGTCGAGATTTAGAAATTG
AGCATTTCCAGCCCAAAGACTTCTTTGAAGTACAGGCTTGGGTTAATCCAGAGAGCAAAGAAGAAAAAACTCCAGAAAAA
TCAACCGCACTTTTCAGTGCCTTATGGCAACCTAGCAAAGCTTGCGAGGATTACCAAGATGATGACGGCAGGGTGCTCTC
TAAAGGATTAGCAGAAAAAGTTGTAAAACGTATTACAAATCAACCTGCAGAAGTCACCGAATACAAAGACGTTCGTGAAA
AAGAAACGGCGCCCTTGCCTTATTCACTTTCTGCGTTACAAATTGATGCAGCAAAGCGATTTGGTATGTCCGCTCAAGCC
GTATTAGATACGTGCCAACGCCTATATGAAACTCATCGTTTGATTACTTATCCGCGTTCTGATTGTCGCTATTTGCCCGA
AGAACATTTTGCCGAACGCCATAATGTATTAAATGCAATTTCAACCCATTGTGAAGCTTATCAAGTCTTACCAAATGTTA
TTTTAACTGAACAGAGAAATCGCTGTTGGAATGATAAAAAAGTAGAGGCTCACCATGCAATCATTCCTACTGCCAAAAAT
CGTCCAGTAAATCTAACACAAGAAGAACGTAATATTTACAGCCTTATCGCTCGCCAATATTTAATGCAATTTTGCCCTGA
CGCAGAATATCGTAAAAGTAAAATCACATTAAATATCGCTGGCGGTACTTTTATAGCTCAAGCACGAAATTTACAAACGG
CTGGGTGGAAAGAATTGTTAGGCAAAGAAGACGACACAGAAAATCAAGAACCCTTATTACCAATAGTAAAGAAAGGACAA
ATTTTGCATTGTGAACGCGGAGAAGTGATGAGTAAAAAAACTCAGCCACCAAAGCCCTTCACTGATGCAACCTTGCTTTC
AGCAATGACGGGCATTGCGCGATTTGTGCAGGATAAAGAACTCAAAAAAATTCTGCGAGAAACCGATGGATTGGGTACAG
AGGCTACTCGCGCTGGCATCATTGAATTACTTTTTAAACGCGGTTTTTTAACTAAAAAAGGGCGAAATATTCATAGCACA
GAAACAGGAAGAATTTTAATTCAGGCATTACCAAATATTGCCACCCAACCAGATATGACGGCTCATTGGGAATCGCAACT
CACAGACATTAGTCAAAAACAAGCAACCTATCAACAATTTATGCATAACTTGAATCAAATATTGCCTGATTTAGTCCGTT
TTGTAGATCTTAATGCATTAAGACAATTAAGCCGCATTAAAATGATTAAATCCGATAGAGCGAAACCTAAAAGTGCGGTA
AAAAAATCGAGTAAATCAAACGGTGAGACTGATTAA

Upstream 100 bases:

>100_bases
CGGTATCCCTGTCGGTGGTGAACTGGAAACTGTGGACGGCACAACGCTTACTCACTCTTTTCTTGGTCGTCGTCAAATCG
ACTAATCTTCTCATACCATT

Downstream 100 bases:

>100_bases
AGTTAAACCAATTAATATTTTTTTTATGCAAAGCACTTGCAAGAAAATTGAATTTTCTATAACATTCACGACCAGTTTTC
TTGCAAAATTGCAAATTAGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVQAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAEKVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQVLPNVILTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
ETGRILIQALPNIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQILPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGETD

Sequences:

>Translated_651_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVQAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAEKVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQVLPNVILTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
ETGRILIQALPNIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQILPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGETD
>Mature_651_residues
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQWRLEHLPIIPEKWQLLPRKE
VKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYANLSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLAT
SALARARADWLYGINMTRAYTIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVQAWVNPESKEEKTPEK
STALFSALWQPSKACEDYQDDDGRVLSKGLAEKVVKRITNQPAEVTEYKDVREKETAPLPYSLSALQIDAAKRFGMSAQA
VLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAISTHCEAYQVLPNVILTEQRNRCWNDKKVEAHHAIIPTAKN
RPVNLTQEERNIYSLIARQYLMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGIIELLFKRGFLTKKGRNIHST
ETGRILIQALPNIATQPDMTAHWESQLTDISQKQATYQQFMHNLNQILPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAV
KKSSKSNGETD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=651, Percent_Identity=24.1167434715822, Blast_Score=120, Evalue=5e-27,
Organism=Escherichia coli, GI1788061, Length=648, Percent_Identity=65.4320987654321, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=557, Percent_Identity=24.5960502692998, Blast_Score=109, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=642, Percent_Identity=22.5856697819315, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323263, Length=599, Percent_Identity=22.3706176961603, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640096, Length=684, Percent_Identity=23.0994152046784, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP3_HAEIN (P43704)

Other databases:

- EMBL:   L42023
- PIR:   G64068
- RefSeq:   NP_438605.1
- ProteinModelPortal:   P43704
- SMR:   P43704
- GeneID:   949541
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0444
- NMPDR:   fig|71421.1.peg.418
- TIGR:   HI_0444
- HOGENOM:   HBG585507
- OMA:   NDVVTWC
- ProtClustDB:   PRK07726
- BioCyc:   HINF71421:HI_0444-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01056

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 74068; Mature: 74068

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLLPRKEVKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
LSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCEEEE
TIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVQAWVNPESKEEKTPEK
EECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCHH
STALFSALWQPSKACEDYQDDDGRVLSKGLAEKVVKRITNQPAEVTEYKDVREKETAPLP
HHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YSLSALQIDAAKRFGMSAQAVLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAI
CCCHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHH
STHCEAYQVLPNVILTEQRNRCWNDKKVEAHHAIIPTAKNRPVNLTQEERNIYSLIARQY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
HHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCC
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGI
EEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IELLFKRGFLTKKGRNIHSTETGRILIQALPNIATQPDMTAHWESQLTDISQKQATYQQF
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MHNLNQILPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAVKKSSKSNGETD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLFIAEKPSLARAIADVLPKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEPDAYDPKFKQ
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
WRLEHLPIIPEKWQLLPRKEVKKQLSVVEKLIHQADTLVNAGDPDREGQLLVDEVFSYAN
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
LSAEKRDKILRCLISDLNPSAVEKAVKKLQPNRNFIPLATSALARARADWLYGINMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCEEEE
TIRGRQTGYDGVLSVGRVQTPVLGLIVRRDLEIEHFQPKDFFEVQAWVNPESKEEKTPEK
EECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCHH
STALFSALWQPSKACEDYQDDDGRVLSKGLAEKVVKRITNQPAEVTEYKDVREKETAPLP
HHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YSLSALQIDAAKRFGMSAQAVLDTCQRLYETHRLITYPRSDCRYLPEEHFAERHNVLNAI
CCCHHHEEHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHH
STHCEAYQVLPNVILTEQRNRCWNDKKVEAHHAIIPTAKNRPVNLTQEERNIYSLIARQY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFCPDAEYRKSKITLNIAGGTFIAQARNLQTAGWKELLGKEDDTENQEPLLPIVKKGQ
HHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCC
ILHCERGEVMSKKTQPPKPFTDATLLSAMTGIARFVQDKELKKILRETDGLGTEATRAGI
EEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IELLFKRGFLTKKGRNIHSTETGRILIQALPNIATQPDMTAHWESQLTDISQKQATYQQF
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MHNLNQILPDLVRFVDLNALRQLSRIKMIKSDRAKPKSAVKKSSKSNGETD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800