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The map label for this gene is ogt [H]

Identifier: 16272351

GI number: 16272351

Start: 423567

End: 424139

Strand: Direct

Name: ogt [H]

Synonym: HI0402

Alternate gene names: 16272351

Gene position: 423567-424139 (Clockwise)

Preceding gene: 16272350

Following gene: 16272352

Centisome position: 23.14

GC content: 39.97

Gene sequence:

>573_bases
GTGTGCTTTTCTTTTTATAAAACAAGGCGAAAAATGACCGCACTTTATTACACTTATTACCCCTCGCCAGTCGGACGGCT
TTTGATTTTATCTGACGGCGAGAGCATTACACATATTGATTTTGAAAAAGAGCAATATGCGCCTAATCCAAAATGGCACA
AGCAAGATGAATTGCCTGTTTTTCAAAAAGTGCGGTTAGCTTTTGAACGATATTTTAATGGCGAAGTTGAGTGTTTTTCA
AACATTCCCTTAAAACCAGAAGGCACCGCATTTCAACAAGCCATTTGGCAAGCCTTGCGAGAAATTGATTATGGCGAGCT
TTCAACTTATGGAGAGCTGGCGTTACGGATTAATAATCCTAAAGCTGTTCGTGCCGTGGGAGGCGCGGTAGGGAGTAATC
CGATTAGTATTATTATTCCTTGTCACCGCATTTTAGGCAAAGATCGTACTTTAACTGGTTTTGGTGGTGGCTTAGAGGCA
AAACGTTTTTTATTGCAGTTAGAGAAAATTCCCTATATTGATAAAGGCACTGAAAACACGAAGCCTCGCTTTTTTAAGAA
ATATCACGAATGA

Upstream 100 bases:

>100_bases
TATACTTCTCAAGCACATGCAAATCTTTACGGTTTAAACTTAAATTATAGTTTCTAATCCGTTAAAAAATTTAGCATAAT
AAAGCACACTTCCACACCAA

Downstream 100 bases:

>100_bases
TTCCACAAACCCTTGAACAATTACTTTCCCAAGCGCAATCAATCGCTGGCTTAACTTTTGGGGAGTTAGCTGATGAACTG
CATATTCCTGTCCCGATTGA

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MCFSFYKTRRKMTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWHKQDELPVFQKVRLAFERYFNGEVECFS
NIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEA
KRFLLQLEKIPYIDKGTENTKPRFFKKYHE

Sequences:

>Translated_190_residues
MCFSFYKTRRKMTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWHKQDELPVFQKVRLAFERYFNGEVECFS
NIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEA
KRFLLQLEKIPYIDKGTENTKPRFFKKYHE
>Mature_190_residues
MCFSFYKTRRKMTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWHKQDELPVFQKVRLAFERYFNGEVECFS
NIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEA
KRFLLQLEKIPYIDKGTENTKPRFFKKYHE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=77, Percent_Identity=42.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787596, Length=101, Percent_Identity=44.5544554455446, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=51.1363636363636, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=42.5, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=42.5, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=130, Percent_Identity=36.1538461538462, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137554, Length=119, Percent_Identity=36.1344537815126, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21904; Mature: 21904

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFSFYKTRRKMTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWHKQDELPV
CCCHHHHHHHHEEEEEEEECCCCCCEEEEEECCCEEEEEECCHHHCCCCCCCCCCCCCCH
FQKVRLAFERYFNGEVECFSNIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNP
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
KAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIPYIDKGTENT
CEEHHHCCCCCCCCEEEEEECHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
KPRFFKKYHE
CCHHHHHHCC
>Mature Secondary Structure
MCFSFYKTRRKMTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWHKQDELPV
CCCHHHHHHHHEEEEEEEECCCCCCEEEEEECCCEEEEEECCHHHCCCCCCCCCCCCCCH
FQKVRLAFERYFNGEVECFSNIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNP
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
KAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIPYIDKGTENT
CEEHHHCCCCCCCCEEEEEECHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
KPRFFKKYHE
CCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]