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The map label for this gene is iscA

Identifier: 16272324

GI number: 16272324

Start: 398354

End: 398677

Strand: Reverse

Name: iscA

Synonym: HI0376

Alternate gene names: 16272324

Gene position: 398677-398354 (Counterclockwise)

Preceding gene: 30995369

Following gene: 16272323

Centisome position: 21.78

GC content: 35.49

Gene sequence:

>324_bases
ATGGGTATTACATTAACAGAAAAAGCAGCGCAGCGAGTGAAAGCCTTTTTGGATAATCGTGGTAAAGGTATTGGATTACG
TTTAGGTGTGAAAACATCAGGTTGTTCTGGCTTAGCTTATGTACTTGAGTTTGTAGATGTTTTAAATTCCGAAGATCAAG
TTTTTGAGCAGTACGGTGTTAATATTATTGTCGATCCAAAAAGTCTCGTTTATCTCAATGGCATTGAGCTGGATTATGTA
AAAGAAGGCCTAAATGAAGGCTTTAAATATAATAATCCAAATGTAAAAGAATCTTGTGGTTGTGGCGAAAGTTTCCACGT
TTAA

Upstream 100 bases:

>100_bases
CAATTAAAGCAGCAATTGCTGATTATAAAGCAAAACAAGGCTAATCATGTGAAAAAGTGCGGTCAATTTTGACCGCATTT
TTAGCGGTGTTGGGGGATAG

Downstream 100 bases:

>100_bases
GGTAATAATCTGATGTTCAATCCTTTTCAAATTTTTGATTTGCCAGTTGATTTTCAGCTGGATGAAAAAGTACTAAATGC
ACGTTATTTAAAATTACAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MGITLTEKAAQRVKAFLDNRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNSEDQVFEQYGVNIIVDPKSLVYLNGIELDYV
KEGLNEGFKYNNPNVKESCGCGESFHV

Sequences:

>Translated_107_residues
MGITLTEKAAQRVKAFLDNRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNSEDQVFEQYGVNIIVDPKSLVYLNGIELDYV
KEGLNEGFKYNNPNVKESCGCGESFHV
>Mature_106_residues
GITLTEKAAQRVKAFLDNRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNSEDQVFEQYGVNIIVDPKSLVYLNGIELDYVK
EGLNEGFKYNNPNVKESCGCGESFHV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=39.622641509434, Blast_Score=99, Evalue=8e-22,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=39.7590361445783, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=70.0934579439252, Blast_Score=159, Evalue=5e-41,
Organism=Escherichia coli, GI1787974, Length=106, Percent_Identity=41.5094339622642, Blast_Score=100, Evalue=2e-23,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=40.9090909090909, Blast_Score=79, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=38.6792452830189, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=38.6792452830189, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=37.7358490566038, Blast_Score=68, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=40, Blast_Score=87, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=39.622641509434, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCA_HAEIN (P44672)

Other databases:

- EMBL:   L42023
- PIR:   E64150
- RefSeq:   NP_438537.1
- ProteinModelPortal:   P44672
- SMR:   P44672
- GeneID:   950630
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0376
- NMPDR:   fig|71421.1.peg.350
- TIGR:   HI_0376
- HOGENOM:   HBG730174
- OMA:   ELAPEDQ
- ProtClustDB:   CLSK808409
- BioCyc:   HINF71421:HI_0376-MONOMER
- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302
- Gene3D:   G3DSA:2.60.300.12
- PANTHER:   PTHR10072
- TIGRFAMs:   TIGR00049
- TIGRFAMs:   TIGR02011

Pfam domain/function: PF01521 Fe-S_biosyn; SSF89360 HesB_yadR_yfhF-like

EC number: NA

Molecular weight: Translated: 11722; Mature: 11591

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITLTEKAAQRVKAFLDNRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNSEDQVFEQYGV
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCC
NIIVDPKSLVYLNGIELDYVKEGLNEGFKYNNPNVKESCGCGESFHV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GITLTEKAAQRVKAFLDNRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNSEDQVFEQYGV
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCC
NIIVDPKSLVYLNGIELDYVKEGLNEGFKYNNPNVKESCGCGESFHV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800