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The map label for this gene is rlmN [H]

Identifier: 16272313

GI number: 16272313

Start: 388752

End: 389924

Strand: Direct

Name: rlmN [H]

Synonym: HI0365

Alternate gene names: 16272313

Gene position: 388752-389924 (Clockwise)

Preceding gene: 16272307

Following gene: 16272314

Centisome position: 21.24

GC content: 39.3

Gene sequence:

>1173_bases
ATGTGTAATAACGAGGCTAAAATGTCAGAATTATTGTCTGTGCAATCAGATGCACCCGCTAAAAAAATTAATCTAATGGA
TTTAACGCGCCAACAAATGCGCGAATTTTTTAAAGAACTCGGCGAAAAACCATTTCGAGCAGATCAGTTAGTTAAATGGA
TTTATCATTTTGGCGAAGATAATTTCGACAATATGACAAACATTAATAAAAAATTACGTGAAAAACTTAAGGCGGTTGCA
GAAATTAAAGCCCCAGAAGTTGCCGTTGAGCAGCGTTCAGCCGATGGCACGATAAAATGGGCAATGCAAGTGGGCGAGCA
GCAAGTTGAAACTGTGTATATTCCCGAAGCAGATCGCGCGACGCTTTGTGTATCTTCCCAAGTTGGCTGTGCCTTAGCTT
GTACCTTCTGTTCAACCGCACAACAAGGCTTTAACCGCAATTTAACGGTTTCAGAAATTATCGGTCAAGTATGGCGAGCC
TCTAAAATTATTGGCAACTTTGGCGTAACTGGCGTTAGACCCATTACAAATGTGGTAATGATGGGAATGGGCGAACCCTT
ATTGAATGTTGCTAATGTTGTTCCTGCAATGGAAATTATGTTAGATGATTTTGCCTACGGTTTATCAAAACGTCGTGTAA
CCTTATCGACTTCTGGTGTTGTGCCAGCATTAGATAATTTGAGCAAAATGATCGATGTGGCTTTGGCTATTTCATTACAT
GCCCCAAACGATGAATTACGTGATGAAATTGTGCCAATCAACAAAAAATACAATATCAAAACATTGATTGACTCGGTAAA
TCGTTATTTAAATGTATCAAATGCAAATCATGGCAAAGTGACGATTGAATATGTGATGCTCGATCATGTAAATGATGGCG
TAGAACACGCTCATCAACTTGCAGAAGTATTAAAAAATACGCCTTGTAAAATTAATTTAATTCCGTGGAATCCGTTCCCA
GAAGCACCTTATGCAAAAAGCTCGAATACGCGTATCGATCGCTTCCAAAAAACCTTAATGGAATATGATTTTACCGTGAT
TATCCGTAAAACCCGTGGAGATGATATTGACGCAGCCTGTGGGCAATTAGCGGGAGATGTTATCGATCGTACTAAACGTA
CGGCAATGAAACGCCAATTTGGACAAAATATTGGGGTGACAGAAGTTAATTAA

Upstream 100 bases:

>100_bases
TTATGCGGTGACAAATCTGTACAATCGCCGTTCATTTTTTAATTGCGAATTTTTCGTTGTGAGTTTTTGATAAAAACACC
TGCACAATGCTATCGCCACA

Downstream 100 bases:

>100_bases
TATTCTAGGTATGTGAATTAATCAAGTTCACATACCCCTATGTTTTCATACAAATTAAAGCATATTAAGTATTTCTTCCT
GCCCAATTAAAAGGAGGAAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MCNNEAKMSELLSVQSDAPAKKINLMDLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLREKLKAVA
EIKAPEVAVEQRSADGTIKWAMQVGEQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRA
SKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDNLSKMIDVALAISLH
APNDELRDEIVPINKKYNIKTLIDSVNRYLNVSNANHGKVTIEYVMLDHVNDGVEHAHQLAEVLKNTPCKINLIPWNPFP
EAPYAKSSNTRIDRFQKTLMEYDFTVIIRKTRGDDIDAACGQLAGDVIDRTKRTAMKRQFGQNIGVTEVN

Sequences:

>Translated_390_residues
MCNNEAKMSELLSVQSDAPAKKINLMDLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLREKLKAVA
EIKAPEVAVEQRSADGTIKWAMQVGEQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRA
SKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDNLSKMIDVALAISLH
APNDELRDEIVPINKKYNIKTLIDSVNRYLNVSNANHGKVTIEYVMLDHVNDGVEHAHQLAEVLKNTPCKINLIPWNPFP
EAPYAKSSNTRIDRFQKTLMEYDFTVIIRKTRGDDIDAACGQLAGDVIDRTKRTAMKRQFGQNIGVTEVN
>Mature_390_residues
MCNNEAKMSELLSVQSDAPAKKINLMDLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLREKLKAVA
EIKAPEVAVEQRSADGTIKWAMQVGEQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRA
SKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDNLSKMIDVALAISLH
APNDELRDEIVPINKKYNIKTLIDSVNRYLNVSNANHGKVTIEYVMLDHVNDGVEHAHQLAEVLKNTPCKINLIPWNPFP
EAPYAKSSNTRIDRFQKTLMEYDFTVIIRKTRGDDIDAACGQLAGDVIDRTKRTAMKRQFGQNIGVTEVN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=78.125, Blast_Score=626, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43598; Mature: 43598

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNNEAKMSELLSVQSDAPAKKINLMDLTRQQMREFFKELGEKPFRADQLVKWIYHFGED
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
NFDNMTNINKKLREKLKAVAEIKAPEVAVEQRSADGTIKWAMQVGEQQVETVYIPEADRA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCHHHEEEEECCCCCCC
TLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGVTGVRPITNVVM
EEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDNLSKMIDVALAISLH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEE
APNDELRDEIVPINKKYNIKTLIDSVNRYLNVSNANHGKVTIEYVMLDHVNDGVEHAHQL
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEEEEECCCHHHHHHHHH
AEVLKNTPCKINLIPWNPFPEAPYAKSSNTRIDRFQKTLMEYDFTVIIRKTRGDDIDAAC
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHH
GQLAGDVIDRTKRTAMKRQFGQNIGVTEVN
HHHHHHHHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MCNNEAKMSELLSVQSDAPAKKINLMDLTRQQMREFFKELGEKPFRADQLVKWIYHFGED
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
NFDNMTNINKKLREKLKAVAEIKAPEVAVEQRSADGTIKWAMQVGEQQVETVYIPEADRA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCHHHEEEEECCCCCCC
TLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGVTGVRPITNVVM
EEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDNLSKMIDVALAISLH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEE
APNDELRDEIVPINKKYNIKTLIDSVNRYLNVSNANHGKVTIEYVMLDHVNDGVEHAHQL
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEEEEECCCHHHHHHHHH
AEVLKNTPCKINLIPWNPFPEAPYAKSSNTRIDRFQKTLMEYDFTVIIRKTRGDDIDAAC
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHH
GQLAGDVIDRTKRTAMKRQFGQNIGVTEVN
HHHHHHHHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA