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The map label for this gene is napD

Identifier: 16272295

GI number: 16272295

Start: 370110

End: 370391

Strand: Direct

Name: napD

Synonym: HI0343

Alternate gene names: 16272295

Gene position: 370110-370391 (Clockwise)

Preceding gene: 16272293

Following gene: 16272296

Centisome position: 20.22

GC content: 35.23

Gene sequence:

>282_bases
ATGAATAATACAAATTTAATATTAGAAAATGCGAGAGATTGGCACGTNGTTGGATTGATCGTGCAAGGTAATCCAAAAAA
ATTATCAGCAATTCAAACCGCACTTTTAGCGATTGAGCATACTGAAATTCCGACTCTTGATGAAAAACTTGGAAAATTTG
TGGTGGTAATGCAATCGAACGATCAGCATCTCTTGCTTGAAAAAATGGAAAGTGTGAAAGATATCGACGGTGTGATTAAT
GTATCGTTGGTGTATCACGAGCAAGATGAACAAAATAAATAG

Upstream 100 bases:

>100_bases
GTAGCACAACCTTTAGTTAATTTCGATGCTTGTAATGGCTGTGGGGCTTGTGTACAAGGCTGCCCCGTGAATGCAATAAC
AATGAATGATCTAAAACAAA

Downstream 100 bases:

>100_bases
TTTTAAAATCAAACGTTTAACGTGGGGGAAAACGCGATGAATTTAAGTCGTCGAGACTTTATGAAAGCCAATGCGGCTAT
GGCAGCCGCAACGGCAGCGG

Product: NapD

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MNNTNLILENARDWHVVGLIVQGNPKKLSAIQTALLAIEHTEIPTLDEKLGKFVVVMQSNDQHLLLEKMESVKDIDGVIN
VSLVYHEQDEQNK

Sequences:

>Translated_93_residues
MNNTNLILENARDWHVVGLIVQGNPKKLSAIQTALLAIEHTEIPTLDEKLGKFVVVMQSNDQHLLLEKMESVKDIDGVIN
VSLVYHEQDEQNK
>Mature_93_residues
MNNTNLILENARDWHVVGLIVQGNPKKLSAIQTALLAIEHTEIPTLDEKLGKFVVVMQSNDQHLLLEKMESVKDIDGVIN
VSLVYHEQDEQNK

Specific function: Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme

COG id: COG3062

COG function: function code P; Uncharacterized protein involved in formation of periplasmic nitrate reductase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788535, Length=81, Percent_Identity=38.2716049382716, Blast_Score=70, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPD_HAEIN (P44651)

Other databases:

- EMBL:   L42023
- PIR:   I64148
- RefSeq:   NP_438507.1
- ProteinModelPortal:   P44651
- SMR:   P44651
- GeneID:   949452
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0343
- NMPDR:   fig|71421.1.peg.321
- TIGR:   HI_0343
- HOGENOM:   HBG466530
- OMA:   IEHTEIP
- ProtClustDB:   CLSK789810
- BioCyc:   HINF71421:HI_0343-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005623

Pfam domain/function: PF03927 NapD

EC number: NA

Molecular weight: Translated: 10516; Mature: 10516

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTNLILENARDWHVVGLIVQGNPKKLSAIQTALLAIEHTEIPTLDEKLGKFVVVMQSN
CCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHEECCCCCCHHHCCCEEEEEEECC
DQHLLLEKMESVKDIDGVINVSLVYHEQDEQNK
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNNTNLILENARDWHVVGLIVQGNPKKLSAIQTALLAIEHTEIPTLDEKLGKFVVVMQSN
CCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHEECCCCCCHHHCCCEEEEEEECC
DQHLLLEKMESVKDIDGVINVSLVYHEQDEQNK
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800