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The map label for this gene is priA

Identifier: 16272291

GI number: 16272291

Start: 365938

End: 368130

Strand: Reverse

Name: priA

Synonym: HI0339

Alternate gene names: 16272291

Gene position: 368130-365938 (Counterclockwise)

Preceding gene: 16272300

Following gene: 161350014

Centisome position: 20.11

GC content: 41.22

Gene sequence:

>2193_bases
ATGAAAATTGTCCGAGTGGCACTGGCTGTGCCGCTTCCTCGCTTATTTGATTATTTCGTCCCCGATGATGTTTCATTACA
AATCGGTATGCGTGTTCTTGTGCCTTTTGGCACTCAAAAACGTGTCGCCATTGTGGCTGATTTTCCGACAAAATCGGACG
TGCCAGAGGATAAACTCAAGGCTATTCTTCAACCATTGGATTTAGCCCCACTTTTTACGCCCATTTATTGGGATTGGCTT
CATTGGGCAGCAAATTATTATCAAGCGGGATTAGGCGATGTGTTATTTCAAGCCTTACCTGTGAAATTGCGTAACGGCGA
AAGTGCGGTAAAAAATGACCGCACTTTTTGGCGAATTACGGATGCGGGGAAAAATGCCCTTAAACAAGGCGAATTAAAAC
GCTCGAAAAAGCAAGCTGAAGCCCTACAATACTTATCTGAAACTGATCTTGAAAAAGGCAATAATGATTTTTCCTCTGCC
ATTTGGTCAGCCTTAAAAGCCAAAGGGTTTATTGAAGAAATTACTATCCAAACAAATCCCCTAAGCTGGCAACAAAGATT
AGGCAATAACCCAATTGTTAATGCTGAAAATCGTTTAACCTTAAATAAGCAGCAAGCCTTAGCCTTCAGCCAACTGCTCT
TCCATTCTGGTTTCAATGTATGGCTGCTCGATGGCGTGACTGGTTCAGGCAAAACAGAAATTTATCTGCAATATATTGAA
GAAATTTTAAAATCGGGCAAACAAGTATTAGTGCTTGTACCCGAAATCGGGCTAACACCACAAACGGTGCAACGTTTCAA
AGTGCGTTTCAATGTAGAAATTGATGTGCTGCATTCTAATTTAACCGATACGCAACGGCTTTATGTATGGGATCGCGCCC
GCTCTGGACAAAGTGCTATTGTGATTGGCACTAGATCGGCATTGTTCACGCAATTTTCTAATCTTGGTGCAATTATTTTG
GACGAGGAACACGATTCGTCTTACAAACAGCAAGACAGTTGGCGTTATCACGCGCGAGACTTGGCGATTGTACTGGCTCA
AAAACTGAACATTTCAGTATTAATGGGTTCTGCTACGCCAAGTTTAGAAAGTATTAATAATGTACAAAACGGCAAATACC
AACATTTAGTTTTATCAAAAAGAGCGGGAAATTCTACCGCACTTCGTCATTTCGTTATTGATTTGAAAAATCAAAATATA
CAAAACGGGCTTTCAAAACCCTTGTTGGAACGAATGAAAGCCCACCTCGAAAAAGGCAATCAAGTGTTGCTTTTCTTGAA
TCGTCGTGGCTTTGCGCCCGTATTGCTTTGCCATGAATGTGGTTGGATCGCACAATGCCCGCATTGTGAAAAACCTTATA
CCTATCATCAGCATCAAAATGTATTGCGTTGTCATCATTGTGGTGCACAGAAAACTATTCCTCGACAATGTGGTGATTGT
GGCTCGACTCATTTAGTCACTACAGGTCTCGGCACAGAACAATTAGAAGAAACCTTGAAAACTCTATTTCCTCATTATTC
TGTGGCTCGAATCGACCGCGATAGCACTTCGCGTAAAGGAAAATTGGAAGGTTATTTAGAAGATATTCAACAAGGCAAAA
GTCAAATTTTGATTGGCACACAAATGCTTGCGAAAGGACATCATTTCCCGAATGTTACTCTCGTTGCCTTAGTGAATGTA
GATAGTGCATTATTTTCACTGGATTTTCGTGCAGAAGAACGTTTAGCTCAACTTTATATTCAGGTTGCTGGACGTGCAGG
ACGTGCCGATAAACAAGGTGAAGTGGTGCTACAAACACATTATCCAGATCATCCTTTGCTTACCACTTTATTGGCAAATG
GCTACCAAGCTTTTGCAAAAGAAACCTTACAATTACGCCATTCAATGGGATTGCCGCCTTTTACTTTTCAGGCGTTAATC
AAAGCACAGGCTCGACATTCTGATCTTGCAGAAAATTGCTTAAGCCAAATTGCAGATTTTTTTCAATCGAAGCAAATTAC
GGGATTACAAATGCTTGGCCCAATGCCTGCACCGTTCAGCAAAAAAGCGGGGCAATATCGCTGGCAGTTATTATTGCAGC
ATCCATCAAGAATGACGTTGCAAAAAGCATTAAGAGAATATCAACAAGCCGAACTAGAAAAAAACAGCCAAGTGCGGTTA
ATTTTGGACGTCGATCCGCAAGACTTATCATAA

Upstream 100 bases:

>100_bases
GGCAAAAGTTTGTGTCATAAAAATCTTGTTCGTGAAAAAATTGGCGGCGATTATACCAATTTTTCTCGTATAATAGGGCA
AATTTTTTCCAAGGTTGATT

Downstream 100 bases:

>100_bases
AAAATGCGGTGAAAATTCACCGCACTTTTATCTCTAAAATCTATTTGAAATCTTCGACATCTCCTAATAGACAGGCATAT
TTCGCTGTATTGGGACTGGA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALKQGELKRSKKQAEALQYLSETDLEKGNNDFSSA
IWSALKAKGFIEEITIQTNPLSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVQRFKVRFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDSSYKQQDSWRYHARDLAIVLAQKLNISVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTSRKGKLEGYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALI
KAQARHSDLAENCLSQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS

Sequences:

>Translated_730_residues
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALKQGELKRSKKQAEALQYLSETDLEKGNNDFSSA
IWSALKAKGFIEEITIQTNPLSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVQRFKVRFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDSSYKQQDSWRYHARDLAIVLAQKLNISVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTSRKGKLEGYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALI
KAQARHSDLAENCLSQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS
>Mature_730_residues
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALKQGELKRSKKQAEALQYLSETDLEKGNNDFSSA
IWSALKAKGFIEEITIQTNPLSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVQRFKVRFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDSSYKQQDSWRYHARDLAIVLAQKLNISVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTSRKGKLEGYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALI
KAQARHSDLAENCLSQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=737, Percent_Identity=52.1031207598372, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=211, Percent_Identity=25.1184834123223, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_HAEIN (P44647)

Other databases:

- EMBL:   L42023
- PIR:   G64062
- RefSeq:   NP_438503.1
- ProteinModelPortal:   P44647
- SMR:   P44647
- GeneID:   950761
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0339
- NMPDR:   fig|71421.1.peg.318
- TIGR:   HI_0339
- HOGENOM:   HBG725563
- OMA:   RMTVHQR
- ProtClustDB:   PRK05580
- BioCyc:   HINF71421:HI_0339-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C

EC number: NA

Molecular weight: Translated: 82337; Mature: 82337

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLK
CCEEEEEHHCCHHHHHHHCCCCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
AILQPLDLAPLFTPIYWDWLHWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRIT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCHHHCCCCEEEEEE
DAGKNALKQGELKRSKKQAEALQYLSETDLEKGNNDFSSAIWSALKAKGFIEEITIQTNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
LSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
CHHHHHCCCCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
EILKSGKQVLVLVPEIGLTPQTVQRFKVRFNVEIDVLHSNLTDTQRLYVWDRARSGQSAI
HHHHCCCEEEEEECCCCCCHHHHHHEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCEE
VIGTRSALFTQFSNLGAIILDEEHDSSYKQQDSWRYHARDLAIVLAQKLNISVLMGSATP
EEECHHHHHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
SLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNIQNGLSKPLLERMKAHLEKGN
CHHHHHHCCCCCEEEEEEECCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
QVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
EEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCHHHCCC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTSRKGKLEGYLEDIQQGKSQILIGT
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEH
QMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTH
HHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
YPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALIKAQARHSDLAENCLSQIADF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
HHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
ILDVDPQDLS
EEECCCCCCC
>Mature Secondary Structure
MKIVRVALAVPLPRLFDYFVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLK
CCEEEEEHHCCHHHHHHHCCCCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
AILQPLDLAPLFTPIYWDWLHWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRIT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCHHHCCCCEEEEEE
DAGKNALKQGELKRSKKQAEALQYLSETDLEKGNNDFSSAIWSALKAKGFIEEITIQTNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
LSWQQRLGNNPIVNAENRLTLNKQQALAFSQLLFHSGFNVWLLDGVTGSGKTEIYLQYIE
CHHHHHCCCCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
EILKSGKQVLVLVPEIGLTPQTVQRFKVRFNVEIDVLHSNLTDTQRLYVWDRARSGQSAI
HHHHCCCEEEEEECCCCCCHHHHHHEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCEE
VIGTRSALFTQFSNLGAIILDEEHDSSYKQQDSWRYHARDLAIVLAQKLNISVLMGSATP
EEECHHHHHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
SLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQNIQNGLSKPLLERMKAHLEKGN
CHHHHHHCCCCCEEEEEEECCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
QVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
EEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCHHHCCC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTSRKGKLEGYLEDIQQGKSQILIGT
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEH
QMLAKGHHFPNVTLVALVNVDSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTH
HHHHCCCCCCCEEEEEEEECCHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
YPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALIKAQARHSDLAENCLSQIADF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
HHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
ILDVDPQDLS
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800