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The map label for this gene is ruvB [H]

Identifier: 16272266

GI number: 16272266

Start: 344054

End: 345061

Strand: Reverse

Name: ruvB [H]

Synonym: HI0312

Alternate gene names: 16272266

Gene position: 345061-344054 (Counterclockwise)

Preceding gene: 16272267

Following gene: 16272261

Centisome position: 18.85

GC content: 42.66

Gene sequence:

>1008_bases
ATGATTGAAGCAGATAGAATTATTAGCGGGCAAGCTAAGGTTGATGAAGATGTTATTGACCGTGCTATTCGTCCTAAACT
ATTAGCAGATTATGTTGGGCAGCCGCAAGTGCGCGAGCAGATGGATATTTTCATCAAGGCAGCCAAACTACGTCAAGATG
CCTTAGATCATTTATTGATTTTTGGCCCTCCTGGCTTGGGAAAAACAACCCTTGCTAATATTGTGGCAAATGAAATGGGC
GTGAATATTCGTACGACATCAGGGCCTGTGTTAGAAAAAGCGGGCGATTTGGCTGCAATGCTAACCAATCTTGAACCGCA
TGATGTGTTATTTATTGATGAAATCCATCGACTTTCCCCTGCGATTGAAGAAGTCCTTTATCCAGCAATGGAAGACTATC
AGTTGGATATTATGATTGGCGAAGGACCCGCTGCTCGTTCCATTAAATTAGATTTGCCCCCTTTTACTTTGGTTGGCGCG
ACCACTAGAGCGGGTTCTTTGACTTCTCCATTGCGTGATCGTTTTGGTATTGTGCAACGTTTGGAGTTTTATTCTGTAGA
AGATTTAACATCTATCGTTGCAAGAAGTGCGGGCTGTTTAAATCTTGAATTAGAACAACAAGCCGCTTTTGAGGTGGCTC
GTCGTTCACGAGGCACGCCTCGAATTGCAAACCGTTTATTACGACGTGTGCGTGATTATGCAGATGTACGTAATGGCGGT
ATTATTTCTATAAATGTGGCAAAACAAGCACTTTCAATGCTAGATGTAGACGATGCTGGATTTGATTATTTAGATAGAAA
GTTGTTGTCTGCAGTTATTGAGCGTTTTGATGGTGGCCCTGTGGGGCTAGATAATCTTGCTGCTGCAATTGGCGAAGAAC
GTGATACCATTGAAGATGTGTTAGAGCCTTATTTGATTCAACAGGGGTTTCTACAAAGAACGCCACGTGGCCGTATTGCA
ACATCGCAAACTTATCGTCATTTTGGTTTGCAGAAATTATCAGACTAG

Upstream 100 bases:

>100_bases
CCTGTAGAAGCAGAAAAAATGGTAAAACGCGTTGCTAAACCTGAATTAACCAGTGAACAAGTTATTCGTGAAGCATTAAA
AGTCGCATTGTAGGAAAAAT

Downstream 100 bases:

>100_bases
ATAAATTTAACCACTAAAAAAGCCTTATTGTACTCAATAAGGCTTTTTTAATTACTCACTATAACTTTTCAAGTTACTTA
ATTTTGCTAAACCCACTTCA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
IISINVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSQTYRHFGLQKLSD

Sequences:

>Translated_335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
IISINVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSQTYRHFGLQKLSD
>Mature_335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
IISINVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSQTYRHFGLQKLSD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=81.5151515151515, Blast_Score=547, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37019; Mature: 37019

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
FGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
IISINVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDV
EEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LEPYLIQQGFLQRTPRGRIATSQTYRHFGLQKLSD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
FGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
IISINVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDV
EEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LEPYLIQQGFLQRTPRGRIATSQTYRHFGLQKLSD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA