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The map label for this gene is dusC

Identifier: 16272228

GI number: 16272228

Start: 303876

End: 304808

Strand: Reverse

Name: dusC

Synonym: HI0270

Alternate gene names: 16272228

Gene position: 304808-303876 (Counterclockwise)

Preceding gene: 16272229

Following gene: 16272223

Centisome position: 16.65

GC content: 40.51

Gene sequence:

>933_bases
GTGCGTGTTATTCTTGCGCCTATGCAAGGGGTTTTAGATCCCTTTGTACGTGAACTTCTCACCGAAGTGAATGACTACGA
TTTATGTATAACAGAATTTGTTCGCGTAGTTGATCAACTTCTCCCTGAAAAAGTATTTTATCGTTTATGCCCTGAATTAA
AAAATCAGGGCTTTACTTCTTCTGGCACGCCTGTGCGAGTGCAGTTGCTAGGGCAGCATCCAAAATGCCTTGCTGAAAAT
GCAATTCGTGCGATCGATCTTGGTTCTCATGGCATTGATTTAAATTGTGGTTGCCCTTCTAAAACAGTGAATGGCAGCAA
TGGTGGTGCGGCATTATTGAAACAGCCTGAATTGATTTATCGTGCCACTCAAGCCTTACGCAGAGCCGTGCCAAGTGAAT
TTCCCGTTAGTGTAAAAGTGCGGTTAGGTTGGGATGATATTTCTCAAGCCTTTGAAATCGCTGATGCGGTAGAGCAAGGC
GGCGCAACCGAAATTACAGTTCATGGACGTACAAAAGCTGATGGTTATCGTGCTGATCGAATTAATTGGAAAAAAATTAG
CGAAGTCCGAGAGCGTTTATCCATTCCAGTTATTGCTAACGGAGAAATTTGGCATTGGCAAGATGGGCAAGATTGCTTAT
CCCAAACAGGTTGTCAGGATTTAATGGTCGGACGAGGCGCGTTGAATATTCCGAATTTAAGTCATGTTCTGAAATCAAAT
GCAGAAAAAATGCCTTGGTATGAGATTCAAAAAATCTTGCAAAAATATGCGAATGTCGAAAATGAATATGACAGCGGTTT
TTATCATGTGGCACGAATTAAACAATGGTTACGTTATTTGAATAAGGAATATAATGAAGCGAACCAAGTGTTTGATAAGA
TTAAGACTTGCCAAACTGCTGAAGATTTGAAATTACGGTTAAATGAGAAATAA

Upstream 100 bases:

>100_bases
CGAAAGGTTTACCGCCAGAAATGATGGAAATGGCAAAAGAAAAAACTCAACAGATTCAAGCCGCTTACGATTTAATTTGT
AAAGCAAAAGGCTGGAAATA

Downstream 100 bases:

>100_bases
AAAACCTGCTAATCAGCAGGTTTTCTTTTTCTAAATTATTTAAAAATTCACCGCACTTTTTAATTTTTTCAGTGCATTAG
TTTCTAATTGACGAATACGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSGTPVRVQLLGQHPKCLAEN
AIRAIDLGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKADGYRADRINWKKISEVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWYEIQKILQKYANVENEYDSGFYHVARIKQWLRYLNKEYNEANQVFDKIKTCQTAEDLKLRLNEK

Sequences:

>Translated_310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSGTPVRVQLLGQHPKCLAEN
AIRAIDLGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKADGYRADRINWKKISEVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWYEIQKILQKYANVENEYDSGFYHVARIKQWLRYLNKEYNEANQVFDKIKTCQTAEDLKLRLNEK
>Mature_310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSGTPVRVQLLGQHPKCLAEN
AIRAIDLGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKADGYRADRINWKKISEVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWYEIQKILQKYANVENEYDSGFYHVARIKQWLRYLNKEYNEANQVFDKIKTCQTAEDLKLRLNEK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=243, Percent_Identity=31.2757201646091, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=321, Percent_Identity=27.7258566978193, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI8923374, Length=265, Percent_Identity=26.7924528301887, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI239788483, Length=229, Percent_Identity=27.9475982532751, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=27.9475982532751, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=303, Percent_Identity=66.3366336633663, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789660, Length=279, Percent_Identity=31.5412186379928, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=24.2138364779874, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=280, Percent_Identity=29.6428571428571, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=171, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=207, Percent_Identity=26.5700483091787, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=165, Percent_Identity=27.8787878787879, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921524, Length=214, Percent_Identity=34.1121495327103, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24585320, Length=245, Percent_Identity=31.8367346938775, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=29.8245614035088, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=29.8245614035088, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSC_HAEIN (P44606)

Other databases:

- EMBL:   L42023
- PIR:   F64146
- RefSeq:   NP_438439.1
- ProteinModelPortal:   P44606
- SMR:   P44606
- GeneID:   950625
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0270
- NMPDR:   fig|71421.1.peg.255
- TIGR:   HI_0270
- HOGENOM:   HBG557545
- OMA:   VGGIDWC
- ProtClustDB:   CLSK789896
- BioCyc:   HINF71421:HI_0270-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 35057; Mature: 35057

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTS
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SGTPVRVQLLGQHPKCLAENAIRAIDLGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIY
CCCCEEEEEECCCCHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCHHHHCCHHHHH
RATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQGGATEITVHGRTKADGYRADR
HHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
INWKKISEVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
CCHHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCC
AEKMPWYEIQKILQKYANVENEYDSGFYHVARIKQWLRYLNKEYNEANQVFDKIKTCQTA
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDLKLRLNEK
HHHHHHCCCC
>Mature Secondary Structure
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTS
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SGTPVRVQLLGQHPKCLAENAIRAIDLGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIY
CCCCEEEEEECCCCHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCHHHHCCHHHHH
RATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQGGATEITVHGRTKADGYRADR
HHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
INWKKISEVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
CCHHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCC
AEKMPWYEIQKILQKYANVENEYDSGFYHVARIKQWLRYLNKEYNEANQVFDKIKTCQTA
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDLKLRLNEK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800