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The map label for this gene is queA [H]

Identifier: 16272205

GI number: 16272205

Start: 273092

End: 274183

Strand: Reverse

Name: queA [H]

Synonym: HI0245

Alternate gene names: 16272205

Gene position: 274183-273092 (Counterclockwise)

Preceding gene: 16272206

Following gene: 16272204

Centisome position: 14.98

GC content: 37.0

Gene sequence:

>1092_bases
ATGCGTGTTTCAGACTTTAACTTTGATTTACCCGATGAATTAATTGCTCGTTATCCTAAAACTGATCGTGTTTCTTGTCG
TTTATTACAGTTAAATGGCGAAAACGGAGAAATTTTTCACCGCACTTTTTCTGATGTATTAGATTTAATTGATGAAGGCG
ATTTGTTGATTTTTAATAATACACGCGTTATTCCTGCAAGAATGTTTGGGCGTAAAGCCAGCGGTGGGAAAATTGAAGTT
TTAGTTGAACGGATGTTAGATGAACATCGTTTCTTAGCGCATATTCGTTCATCGAAATCCCCTAAAGAAGGGGCTGAATT
ATTTTTAGGGGAAGATAAATTAGGCGAAAATAATGGCATTAAAGCGGTAATGAAAGCGCGTCATGGTGCATTGTTTGAAG
TGGAATTAAGTGATAAATCAACCGCTCTTTTAGATGTGTTGCAAACCATTGGGCATATGCCATTGCCGCCTTATATTGAC
CGCCCAGATGAAGAAGCCGATCAAGAATGTTATCAAACGGTTTATAGTAAAGTACCAGGTGCGGTAGCAGCACCAACAGC
AGGCTTACATTTTGATGAAAATTTACTTGAAAAACTCAAAGCAAAGGGTGTGAATTTTGAATTTGTTACATTACATGTAG
GCGCAGGAACATTCCAGCCTGTTCGTGTGGAAAATATTGAAGATCACGTAATGCATGCGGAGTATGTGGAAGTTTCTCAA
GAAGTCTGTAATGCGATTATCGCAACAAAAAAAGCGGGCAAACGTGTAATTGCAGTGGGAACTACTTCTGTTCGTTCTAT
TGAAAGTGCGGCACTTTCTGCGGAAGAATTTGGCAATCCAGATTTAATTGAGCCTTATTTTTCTGATACCTCTATTTTTA
TTTATCCAGGGAAAAAATTCCGAGTGGTGGATTGCTTAATTACTAATTTCCACTTACCTGAAAGCACTTTGATTATGTTA
GTTTCAGCTTTTGCTGGTTATAAAAATACCATGAATGCATATAAGCATGCAGTAAAAGAAAAATATCGTTTCTTTAGTTA
TGGCGATGCGATGTTTATTAATAAAAATTCTAATGTGAGAGGATTGGAATAA

Upstream 100 bases:

>100_bases
TTGCTGGTTATTTTATCTTCCCCGAGGGGAGATTTTTTCTTGAGATCATTTTCAATTCAAACTAGACTTACCGCCTATTT
TATTTAACCTAAATTTTGTT

Downstream 100 bases:

>100_bases
GTTGTTTGACTTGTAATTAGTCAGCACCTACAATAAAGAACAAGAGATGGCGGTAACCATCTCTTGTCGGCATCAACTAC
TAATAGCCAGTGCTACTTTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADQECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFINKNSNVRGLE

Sequences:

>Translated_363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADQECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFINKNSNVRGLE
>Mature_363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADQECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFINKNSNVRGLE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=71.0674157303371, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40630; Mature: 40630

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKIEVLVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGI
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCH
KAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYIDRPDEEADQECYQTVYSKVPG
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHH
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKF
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCE
RVVDCLITNFHLPESTLIMLVSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFINKNSNVR
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCC
GLE
CCC
>Mature Secondary Structure
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKIEVLVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGI
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCH
KAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYIDRPDEEADQECYQTVYSKVPG
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHH
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKF
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCE
RVVDCLITNFHLPESTLIMLVSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFINKNSNVR
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCC
GLE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA