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The map label for this gene is birA

Identifier: 16272182

GI number: 16272182

Start: 247910

End: 248818

Strand: Reverse

Name: birA

Synonym: HI0220.1

Alternate gene names: 16272182

Gene position: 248818-247910 (Counterclockwise)

Preceding gene: 16272186

Following gene: 30995363

Centisome position: 13.6

GC content: 34.54

Gene sequence:

>909_bases
ATGATGAATTTTACGTTATTGACATATTTAGCGGATTGTCAGCCGAAAGTGCGGTCTGAATTGGAAAAATTTTCTAAAAA
TCTAGAGGAAGATATTCAACAATTACGTGAAATTGGTTTGGATATTCTCGTTGATGGACAAGATTATCGCTTAGTGCCAA
TGCTTCCTTTATTAAATCCTCAGCAAATTTCAACCGCACTTTTTCCTTATAGTATCCATTATCAGCCGATTATTTCCTCT
ACAAATGAATGGATACTACAAAATATTCTTTCATTAAAAAAAGGCGATCTTTGTGTGGCTGAATATCAAACTGCAGGACG
AGGTCGGCGTGGTCGCCAATGGTTATCGCCTTTTGCCGGGCAAATCATGTTTAGTTTTTATTGGGCATTTGATCCTAAAA
AATCAATTGAGGGGTTAAGTTTAGTCATCGGTTTGGCGATTGCGGAAGTGCTAAATGTACAAGTGAAATGGCCAAATGAT
ATTTTGTTTGATGAAAGAAAGTTAGGTGGCATTTTAGTTGAAATTGCTAATCATAAAAATGGCATGCTCAATTTAGTGAT
TGGCATAGGTATTAATGTGTCGTTGTCAAAACAAACAGAAATTAGTCAGCCTTATGCGGAAGTGTGTGAAATTGATCCTG
ATGTAGAGCGACAAACTTTATTACCCAAACTTATACAACATTTATATACACGTTTAAATATCTTTGAACAAAATGGTATT
GATGAGGAATTTCAACAAGCATGGCAATCATATAATGCGTTTTCAAATAGTGAAATAAATGTGCTTACTGAGCAAGGCGT
TATTTCAGGTATTGAACAAGGCATAGATGAACGTGGTTATTTAAAAGTATTATGTGGCAATAAAATTCAGATGTTTAATG
GTGGAGAAGTTTCATTACGTAAGAAATAA

Upstream 100 bases:

>100_bases
AAAAAATATTGCGACGAGATTATACAGATTTCACTTTATTTTGAAAACGAAATTTTAGGATTTATGCTATGATTTTCATA
CTATTTGCCATAAGGAATGA

Downstream 100 bases:

>100_bases
TAGCAATAAAAGTTTGACTATTCTAACCGCACTTTTTAATAAAATGTTTATATTTCCATCAAAAGATCATTTTTTTAAAT
ATTTTTACAAAAAAGTACTT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MMNFTLLTYLADCQPKVRSELEKFSKNLEEDIQQLREIGLDILVDGQDYRLVPMLPLLNPQQISTALFPYSIHYQPIISS
TNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPND
ILFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGI
DEEFQQAWQSYNAFSNSEINVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK

Sequences:

>Translated_302_residues
MMNFTLLTYLADCQPKVRSELEKFSKNLEEDIQQLREIGLDILVDGQDYRLVPMLPLLNPQQISTALFPYSIHYQPIISS
TNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPND
ILFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGI
DEEFQQAWQSYNAFSNSEINVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK
>Mature_302_residues
MMNFTLLTYLADCQPKVRSELEKFSKNLEEDIQQLREIGLDILVDGQDYRLVPMLPLLNPQQISTALFPYSIHYQPIISS
TNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPND
ILFDERKLGGILVEIANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGI
DEEFQQAWQSYNAFSNSEINVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLRKK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family

Homologues:

Organism=Escherichia coli, GI1790408, Length=284, Percent_Identity=39.7887323943662, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI212645369, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32563942, Length=218, Percent_Identity=27.9816513761468, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32563944, Length=218, Percent_Identity=27.9816513761468, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIRA_HAEIN (P46363)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_438391.1
- ProteinModelPortal:   P46363
- GeneID:   951136
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0220.1
- NMPDR:   fig|71421.1.peg.209
- TIGR:   HI_0220.1
- HOGENOM:   HBG750923
- OMA:   ISSTNEW
- ProtClustDB:   PRK11886
- BioCyc:   HINF71421:HI_0220.1-MONOMER
- BRENDA:   6.3.4.15
- GO:   GO:0006350
- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988
- PANTHER:   PTHR12835
- TIGRFAMs:   TIGR00121
- TIGRFAMs:   TIGR00122

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; SSF50037 Transcr_rep_C

EC number: =6.3.4.15

Molecular weight: Translated: 34342; Mature: 34342

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNFTLLTYLADCQPKVRSELEKFSKNLEEDIQQLREIGLDILVDGQDYRLVPMLPLLNP
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCH
QQISTALFPYSIHYQPIISSTNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAG
HHHHHHHCCEEEEECHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHH
QIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDILFDERKLGGILVEIANHKN
HHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHCEEEEEEECCCC
GMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGI
CEEEEEEEECCEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DEEFQQAWQSYNAFSNSEINVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLR
CHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCEEEE
KK
CC
>Mature Secondary Structure
MMNFTLLTYLADCQPKVRSELEKFSKNLEEDIQQLREIGLDILVDGQDYRLVPMLPLLNP
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCH
QQISTALFPYSIHYQPIISSTNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAG
HHHHHHHCCEEEEECHHHCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHH
QIMFSFYWAFDPKKSIEGLSLVIGLAIAEVLNVQVKWPNDILFDERKLGGILVEIANHKN
HHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHCEEEEEEECCCC
GMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNIFEQNGI
CEEEEEEEECCEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DEEFQQAWQSYNAFSNSEINVLTEQGVISGIEQGIDERGYLKVLCGNKIQMFNGGEVSLR
CHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCEEEE
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800