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The map label for this gene is miaA [H]

Identifier: 16272042

GI number: 16272042

Start: 72111

End: 73046

Strand: Direct

Name: miaA [H]

Synonym: HI0068

Alternate gene names: 16272042

Gene position: 72111-73046 (Clockwise)

Preceding gene: 16272041

Following gene: 16272043

Centisome position: 3.94

GC content: 43.06

Gene sequence:

>936_bases
ATGAAACCAACCGCAATTTTTTTAATGGGCCCAACAGCCTCAGGTAAAACAGATTTAGCCATTCAACTACGAAGCCAACT
TCCAGTCGAAGTGATTAGTGTGGATTCAGCTCTTATTTATAAGGGAATGGATATTGGCACAGCGAAACCATCAAAAGAAG
AACTTGCCCTTGCGCCTCACCGTTTAATTGATATTTTAGATCCATCAGAAAGCTATTCCGCAATGAATTTCCGCGATGAT
GCGCTACGCGAAATGGCGGATATTACCGCACAAGGCAAAATTCCGTTACTAGTGGGCGGCACGATGTTGTATTACAAAGC
CTTAATCGAAGGGCTTTCGCCCTTGCCTTCTGCTGATGAAAATATTCGCGCCGAGCTTGAACAAAAAGCAGCTCAACAAG
GTTGGGCAGCATTGCATACAGAACTCGCCAAAATCGATCCCATTTCTGCCGCCCGAATTAATCCCAGTGATTCTCAACGA
ATCAATCGCGCTTTAGAAGTTTTTTACATCACAGGAAAATCACTCACAGAGCTGACTGAAGAAAAAGGCGAAGCCTTGCC
TTATGATTTTGTGCAATTTGCAATTGCGCCGCAAGATCGCCATGTTTTGCATGAACGCATCGAACAACGCTTTCATAAAA
TGATTGAACTAGGCTTTCAAGCAGAAGTGGAAAAACTTTATGCGCGTGGCGATTTAAATATTAATCTGCCGTCAATTCGC
TGTGTAGGCTATCGTCAAATGTGGGAATATTTGCAAGGTGATTACGCTTACGAGGAAATGATTTTCCGTGGTATTTGCGC
TACGCGCCAACTTGCAAAACGCCAGCTTACTTGGCTGCGTGGTTGGAAAACACCAATACAATGGCTAGATAGTTTACAAC
CTCAACAAGCGAAAGAAACTGTATTACGTCACTTAGATTCTTATCAAAAAGGTTAG

Upstream 100 bases:

>100_bases
GAGACGGAACGTTTACTCACGCAAACAAATCGCACAGCATTCACCCAATTACTCAAACCTGTTAATTGGCAGCCTTTATT
AGACGAAATTTAAATTCTTC

Downstream 100 bases:

>100_bases
AAAAAATGACCGCACTTTCTGCCCTCATCGAACAAAAATTACAATCTCTTGGCACAATCCTGTGCCAATCTTTTCCAGAA
CTTTCCCCAGAAAAAATTCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKPTAIFLMGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPHRLIDILDPSESYSAMNFRDD
ALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADENIRAELEQKAAQQGWAALHTELAKIDPISAARINPSDSQR
INRALEVFYITGKSLTELTEEKGEALPYDFVQFAIAPQDRHVLHERIEQRFHKMIELGFQAEVEKLYARGDLNINLPSIR
CVGYRQMWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKETVLRHLDSYQKG

Sequences:

>Translated_311_residues
MKPTAIFLMGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPHRLIDILDPSESYSAMNFRDD
ALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADENIRAELEQKAAQQGWAALHTELAKIDPISAARINPSDSQR
INRALEVFYITGKSLTELTEEKGEALPYDFVQFAIAPQDRHVLHERIEQRFHKMIELGFQAEVEKLYARGDLNINLPSIR
CVGYRQMWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKETVLRHLDSYQKG
>Mature_311_residues
MKPTAIFLMGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPHRLIDILDPSESYSAMNFRDD
ALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADENIRAELEQKAAQQGWAALHTELAKIDPISAARINPSDSQR
INRALEVFYITGKSLTELTEEKGEALPYDFVQFAIAPQDRHVLHERIEQRFHKMIELGFQAEVEKLYARGDLNINLPSIR
CVGYRQMWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKETVLRHLDSYQKG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=29.6774193548387, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790613, Length=301, Percent_Identity=69.7674418604651, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25144712, Length=297, Percent_Identity=31.3131313131313, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=30.5084745762712, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24649830, Length=332, Percent_Identity=27.710843373494, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35205; Mature: 35205

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTAIFLMGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPH
CCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCHHHHCCCCCCCCCCCCHHHHHHCHH
RLIDILDPSESYSAMNFRDDALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADE
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCCH
NIRAELEQKAAQQGWAALHTELAKIDPISAARINPSDSQRINRALEVFYITGKSLTELTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHH
EKGEALPYDFVQFAIAPQDRHVLHERIEQRFHKMIELGFQAEVEKLYARGDLNINLPSIR
HHCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCEE
CVGYRQMWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKET
EHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
VLRHLDSYQKG
HHHHHHHHCCC
>Mature Secondary Structure
MKPTAIFLMGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPH
CCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCHHHHCCCCCCCCCCCCHHHHHHCHH
RLIDILDPSESYSAMNFRDDALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADE
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCCH
NIRAELEQKAAQQGWAALHTELAKIDPISAARINPSDSQRINRALEVFYITGKSLTELTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHH
EKGEALPYDFVQFAIAPQDRHVLHERIEQRFHKMIELGFQAEVEKLYARGDLNINLPSIR
HHCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCEE
CVGYRQMWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKET
EHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
VLRHLDSYQKG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA