BacMap

Definition	Haemophilus influenzae Rd KW20 chromosome, complete genome.
Accession	NC_000907
Length	1,830,138

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The map label for this gene is idnO [C]

Identifier: 16272023

GI number: 16272023

Start: 48991

End: 49848

Strand: Reverse

Name: idnO [C]

Synonym: HI0048

Alternate gene names: 16272023

Gene position: 49848-48991 (Counterclockwise)

Preceding gene: 16272024

Following gene: 16272022

Centisome position: 2.72

GC content: 34.38

Gene sequence:

>858_bases
ATGGAGTTTACTATGAATATTGCAGCAAACCATAACTTAGAAAATAAACTAATTATAATCACGGGGGCTGGCGGAGTGTT
ATGTTCATTTCTCGCTAAGCAATTAGCGTATACTAAAGCTAATATTGCCTTGTTAGATTTGAATTTCGAGGCTGCAGACA
AAGTAGCAAAGGAAATTAACCAATCAGGAGGAAAAGCAAAAGCCTATAAAACTAATGTGTTAGAACTTGAAAATATTAAA
GAAGTGAGAAATCAAATTGAAACCGATTTTGGCACTTGTGATATTTTAATTAATGGTGCTGGTGGGAATAATCCAAAAGC
GACAACAGATAATGAGTTCCATCAATTTGATTTAAATGAAACGACAAGAACATTCTTTGATTTAGATAAATCTGGTATAG
AATTTGTATTTAATCTCAATTACTTAGGTTCATTATTACCAACCCAAGTTTTTGCAAAAGATATGCTTGGTAAACAAGGG
GCAAATATTATTAATATTTCCAGTATGAATGCCTTTACGCCACTCACAAAGATTCCTGCTTACTCTGGTGCAAAAGCTGC
AATCAGTAATTTTACTCAATGGCTTGCTGTATATTTCTCGAAAGTTGGTATTCGTTGTAATGCTATCGCACCAGGATTTT
TAGTTAGTAATCAAAACCTCGCTTTATTATTTGATACTGAAGGAAAACCAACAGATAGAGCAAATAAAATCCTCACTAAC
ACACCAATGGGACGTTTTGGTGAGTCAGAAGAATTGCTCGGTGCATTACTTTTCTTAATAGATGAAAATTATTCTGCTTT
TGTCAATGGCGTGGTTTTACCTGTTGATGGCGGTTTTTCTGCGTATAGTGGAGTCTAA

Upstream 100 bases:

>100_bases
GTTGTCAGCAAGGCAATCGTATTGCTGGTATCGTCATTCAACATAAAGGTGCGATTATAGATAAAGTCGCAATTTCTCAT
CTTCAATCTGAATTTAATTA

Downstream 100 bases:

>100_bases
TAGGAGCAAATTAAAAAAATGTCATACACAACTCAACAAATTATTGAAAAACTTCGTGAACTCAAAATTGTGCCTGTAAT
TGCGTTGGATAATGCAGACG

Product: D-mannonate oxidoreductase

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEINQSGGKAKAYKTNVLELENIK
EVRNQIETDFGTCDILINGAGGNNPKATTDNEFHQFDLNETTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQG
ANIINISSMNAFTPLTKIPAYSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKILTN
TPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV

Sequences:

>Translated_285_residues
MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEINQSGGKAKAYKTNVLELENIK
EVRNQIETDFGTCDILINGAGGNNPKATTDNEFHQFDLNETTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQG
ANIINISSMNAFTPLTKIPAYSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKILTN
TPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV
>Mature_285_residues
MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEINQSGGKAKAYKTNVLELENIK
EVRNQIETDFGTCDILINGAGGNNPKATTDNEFHQFDLNETTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQG
ANIINISSMNAFTPLTKIPAYSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKILTN
TPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI40254992, Length=254, Percent_Identity=25.1968503937008, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI15277342, Length=280, Percent_Identity=27.1428571428571, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI66933014, Length=274, Percent_Identity=28.4671532846715, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705925, Length=159, Percent_Identity=28.9308176100629, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI10190704, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI31542939, Length=207, Percent_Identity=27.0531400966184, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790717, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789208, Length=271, Percent_Identity=27.6752767527675, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787905, Length=269, Percent_Identity=28.6245353159851, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787335, Length=268, Percent_Identity=27.2388059701493, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788459, Length=234, Percent_Identity=28.6324786324786, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082160, Length=269, Percent_Identity=27.5092936802974, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082100, Length=276, Percent_Identity=26.4492753623188, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367175, Length=236, Percent_Identity=26.271186440678, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789378, Length=269, Percent_Identity=26.3940520446097, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789057, Length=273, Percent_Identity=24.5421245421245, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=161, Percent_Identity=32.9192546583851, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536025, Length=269, Percent_Identity=26.0223048327138, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536651, Length=268, Percent_Identity=25.7462686567164, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=251, Percent_Identity=26.2948207171315, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=268, Percent_Identity=26.1194029850746, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=269, Percent_Identity=26.3940520446097, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=281, Percent_Identity=25.6227758007117, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=266, Percent_Identity=27.4436090225564, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17508895, Length=223, Percent_Identity=26.9058295964126, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=281, Percent_Identity=27.0462633451957, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=213, Percent_Identity=29.5774647887324, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=197, Percent_Identity=30.9644670050761, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=297, Percent_Identity=24.9158249158249, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=283, Percent_Identity=25.4416961130742, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=286, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=275, Percent_Identity=25.0909090909091, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=203, Percent_Identity=28.0788177339901, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=283, Percent_Identity=25.0883392226148, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=269, Percent_Identity=22.6765799256506, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=265, Percent_Identity=29.4339622641509, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=279, Percent_Identity=27.9569892473118, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=274, Percent_Identity=25.5474452554745, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=271, Percent_Identity=25.0922509225092, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI23397609, Length=269, Percent_Identity=28.2527881040892, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357041, Length=273, Percent_Identity=24.9084249084249, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=277, Percent_Identity=27.0758122743682, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571526, Length=273, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639444, Length=270, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644337, Length=271, Percent_Identity=28.7822878228782, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24643142, Length=270, Percent_Identity=28.1481481481481, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355319, Length=271, Percent_Identity=24.3542435424354, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24649181, Length=226, Percent_Identity=25.6637168141593, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640492, Length=176, Percent_Identity=24.4318181818182, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y048_HAEIN (P44481)

Other databases:

- EMBL:   L42023
- PIR:   B64045
- RefSeq:   NP_438221.1
- ProteinModelPortal:   P44481
- SMR:   P44481
- GeneID:   950942
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0048
- NMPDR:   fig|71421.1.peg.47
- TIGR:   HI_0048
- HOGENOM:   HBG750976
- OMA:   CGYMAKE
- ProtClustDB:   PRK08277
- BioCyc:   HINF71421:HI_0048-MONOMER
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 30907; Mature: 30907

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 181-181 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEIN
CEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHH
QSGGKAKAYKTNVLELENIKEVRNQIETDFGTCDILINGAGGNNPKATTDNEFHQFDLNE
HCCCCCEEEEECCEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCH
TTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQGANIINISSMNAFTPLTKIPA
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCC
YSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKILTN
CCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCEEEEECCCCCCCHHHCEEECC
TPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV
CCCCCCCCHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHCCCC
>Mature Secondary Structure
MEFTMNIAANHNLENKLIIITGAGGVLCSFLAKQLAYTKANIALLDLNFEAADKVAKEIN
CEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHH
QSGGKAKAYKTNVLELENIKEVRNQIETDFGTCDILINGAGGNNPKATTDNEFHQFDLNE
HCCCCCEEEEECCEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCH
TTRTFFDLDKSGIEFVFNLNYLGSLLPTQVFAKDMLGKQGANIINISSMNAFTPLTKIPA
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHCCC
YSGAKAAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNLALLFDTEGKPTDRANKILTN
CCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCEEEEECCCCCCCHHHCEEECC
TPMGRFGESEELLGALLFLIDENYSAFVNGVVLPVDGGFSAYSGV
CCCCCCCCHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023