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The map label for this gene is xthA

Identifier: 16272016

GI number: 16272016

Start: 43660

End: 44463

Strand: Reverse

Name: xthA

Synonym: HI0041

Alternate gene names: 16272016

Gene position: 44463-43660 (Counterclockwise)

Preceding gene: 16272017

Following gene: 16272015

Centisome position: 2.43

GC content: 37.94

Gene sequence:

>804_bases
ATGAAATTTATTTCTTTCAATATAAATGGGTTACGTGCTCGCCCACATCAACTTGAAGCCATTATTGAAAAATATCAGCC
AGATGTGATTGGCTTACAGGAAATTAAAGTTGCTGACGAGGCTTTTCCCTATGAAATTACAGAAAACTTAGGTTATCACG
TATTTCATCACGGTCAGAAAGGTCATTATGGCGTGGCATTATTGACTAAACAAGAACCTAAAGTGATTCGTCGTGGTTTT
CCAACTGACAATGAAGATGCACAAAAACGCATTATTATGGCTGATCTGGAAACTGAATTTGGCTTATTGACTGTCATTAA
TGGCTATTTCCCACAGGGGGAAAGCCGTGCGCACGAGACTAAATTTCCAGCTAAAGAGAAATTTTATGCAGATCTTCAAC
AGTATTTAGAAAAAGAACACGACAAATCGAATCCGATTTTAATTATGGGCGATATGAATATTAGCCCAAGTGATTTAGAT
ATTGGTATCGGAGATGAAAATCGTAAACGTTGGTTACGCACAGGGAAATGTTCTTTCTTACCGGAAGAACGAGCGTGGTA
TCAACGTTTGTATGATTACGGATTAGAAGACAGTTTTCGTAAGCTGAACCCAACAGCAAATGATAAATTCTCGTGGTTTG
ATTACCGTTCTAAAGGTTTTGATGATAATCGAGGTTTACGAATTGATCATATTTTGGTAAGCCAAAAATTAGCTGAACGA
TGTGTCGATGTGGGCATTGCATTAGATATTCGAGCAATGGAAAAACCGTCTGATCACGCACCGATTTGGGCTGAATTTAA
ATAG

Upstream 100 bases:

>100_bases
CAGTATTTGAGCAAATTCAGGTTTATCTGACAAAGCCGTTTTTATCAAAATAGAAATCTCTGTATAATAAAGCCAATTTC
ACTCTTTAATAGGAACAAAA

Downstream 100 bases:

>100_bases
GAAAGCGAAATGGATATGGCAAATTGGCAGAATTATCGTTCTACTGTGAATGGTATGCCTGCCGTGTTTACCGCAAATAT
TGAAGATGTTGAAAAATATC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKVIRRGF
PTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK

Sequences:

>Translated_267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKVIRRGF
PTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK
>Mature_267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKVIRRGF
PTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=27.1375464684015, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=27.1375464684015, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=27.1375464684015, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=71.9101123595506, Blast_Score=413, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=26.1194029850746, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=30.9433962264151, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=30.9433962264151, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EX3_HAEIN (P44318)

Other databases:

- EMBL:   L42023
- PIR:   H64044
- RefSeq:   NP_438214.1
- ProteinModelPortal:   P44318
- SMR:   P44318
- GeneID:   950938
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0041
- NMPDR:   fig|71421.1.peg.40
- TIGR:   HI_0041
- HOGENOM:   HBG704164
- OMA:   MADLETE
- ProtClustDB:   PRK11756
- BioCyc:   HINF71421:HI_0041-MONOMER
- BRENDA:   3.1.11.2
- GO:   GO:0005622
- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808
- PANTHER:   PTHR22748
- TIGRFAMs:   TIGR00195
- TIGRFAMs:   TIGR00633

Pfam domain/function: PF03372 Exo_endo_phos; SSF56219 Exo_endo_phos

EC number: =3.1.11.2

Molecular weight: Translated: 31025; Mature: 31025

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00726 AP_NUCLEASE_F1_1; PS00727 AP_NUCLEASE_F1_2; PS00728 AP_NUCLEASE_F1_3; PS51435 AP_NUCLEASE_F1_4

Important sites: ACT_SITE 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQK
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECHHHEEECCCCCCCHHHHCCCEEEEECCCC
GHYGVALLTKQEPKVIRRGFPTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHET
CCEEEEEEECCCCHHHHCCCCCCCCHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
KFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCC
PEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
CVDVGIALDIRAMEKPSDHAPIWAEFK
HHHCCEEEEEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQK
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECHHHEEECCCCCCCHHHHCCCEEEEECCCC
GHYGVALLTKQEPKVIRRGFPTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHET
CCEEEEEEECCCCHHHHCCCCCCCCHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
KFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCC
PEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
CVDVGIALDIRAMEKPSDHAPIWAEFK
HHHCCEEEEEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800