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The map label for this gene is ada [H]

Identifier: 162447955

GI number: 162447955

Start: 1138568

End: 1139035

Strand: Direct

Name: ada [H]

Synonym: ACL_1101

Alternate gene names: 162447955

Gene position: 1138568-1139035 (Clockwise)

Preceding gene: 162447948

Following gene: 162447956

Centisome position: 76.06

GC content: 32.26

Gene sequence:

>468_bases
ATGTTGTATTATGATTTCATAAGCTATGGTGATTGGACCTTTACCATTAAAGTGTCCAAAAACGGTTTAAAATCTATTGA
TATTTATAATCATCAAGACCTTTCAAATTTAACCTTAAACAAAGAAATAACTGCACCGTATATTCAAATACTTGATAACT
ATTTTAAAGGTATACCCATACCTAAAGATATTAAACTTGATTTAGAAGGTAGTTCATTTGAAAAATTAGTGTGGCAACAA
TTAATTGAGATACCTTACGGAAAGACTGCATCCTATAAAGATATTGCAGTTAGAATTTCAAACCCACGAGCATATCAAGC
CGTTGGTAATGCGGTGGGCAGTAATCCTTTATTGATCGTCATACCTTGCCATAGGGTGATCAAATCAGATGGCAGGTTAG
GTGGATTCTCATCAGATATTCAGTTAAAAATAGATTTATTACATAAAGAAGGGGGAAAGTATTTATGA

Upstream 100 bases:

>100_bases
TTTTTTCATGTCGAATTCCCTCTTTTGCGAATCATTTGCAAAAGTATTATATCACACAAAATAGACTTTGCAAACGATTT
GCAAAAGAAAGGTGCATGCA

Downstream 100 bases:

>100_bases
AGAAACTTTATGATTTTTATCTAAACCAATTGAAACATACCCATTATGTTGAACTAAATTTTGAGCAGTTTGGAGATATG
CTTACTAATTTAGAATATAT

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MLYYDFISYGDWTFTIKVSKNGLKSIDIYNHQDLSNLTLNKEITAPYIQILDNYFKGIPIPKDIKLDLEGSSFEKLVWQQ
LIEIPYGKTASYKDIAVRISNPRAYQAVGNAVGSNPLLIVIPCHRVIKSDGRLGGFSSDIQLKIDLLHKEGGKYL

Sequences:

>Translated_155_residues
MLYYDFISYGDWTFTIKVSKNGLKSIDIYNHQDLSNLTLNKEITAPYIQILDNYFKGIPIPKDIKLDLEGSSFEKLVWQQ
LIEIPYGKTASYKDIAVRISNPRAYQAVGNAVGSNPLLIVIPCHRVIKSDGRLGGFSSDIQLKIDLLHKEGGKYL
>Mature_155_residues
MLYYDFISYGDWTFTIKVSKNGLKSIDIYNHQDLSNLTLNKEITAPYIQILDNYFKGIPIPKDIKLDLEGSSFEKLVWQQ
LIEIPYGKTASYKDIAVRISNPRAYQAVGNAVGSNPLLIVIPCHRVIKSDGRLGGFSSDIQLKIDLLHKEGGKYL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=116, Percent_Identity=35.3448275862069, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788542, Length=92, Percent_Identity=42.3913043478261, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=40.1960784313725, Blast_Score=80, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=43.3734939759036, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=43.3734939759036, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=129, Percent_Identity=34.8837209302326, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17525; Mature: 17525

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYYDFISYGDWTFTIKVSKNGLKSIDIYNHQDLSNLTLNKEITAPYIQILDNYFKGIPI
CEEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCC
PKDIKLDLEGSSFEKLVWQQLIEIPYGKTASYKDIAVRISNPRAYQAVGNAVGSNPLLIV
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEE
IPCHRVIKSDGRLGGFSSDIQLKIDLLHKEGGKYL
ECCHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MLYYDFISYGDWTFTIKVSKNGLKSIDIYNHQDLSNLTLNKEITAPYIQILDNYFKGIPI
CEEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCC
PKDIKLDLEGSSFEKLVWQQLIEIPYGKTASYKDIAVRISNPRAYQAVGNAVGSNPLLIV
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEE
IPCHRVIKSDGRLGGFSSDIQLKIDLLHKEGGKYL
ECCHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA