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The map label for this gene is nqrF [H]

Identifier: 162447824

GI number: 162447824

Start: 994733

End: 995809

Strand: Direct

Name: nqrF [H]

Synonym: ACL_0970

Alternate gene names: 162447824

Gene position: 994733-995809 (Clockwise)

Preceding gene: 162447823

Following gene: 162447852

Centisome position: 66.45

GC content: 34.26

Gene sequence:

>1077_bases
ATGGATCCATTATATACACCAATAATTCTTATTGGTTTACTCATCGTTGTTACACTTGTAGTTATCTTAGTTGATAAATT
ACTCGGTGGTGGTGGTGAACGTAAAATCACTGTTAATAAAGATAATGTCATTACAATCTCTGGTAGAGAAACAGCATTAA
ATGCATTAACTAATAATAAAATTTTCTTACCTTCTTCTTGTGGTGGTAAAGCTACTTGTGGTACATGTAAATTTAGATTA
GTTGACTGGCATGAAGCTCCAAAACCTACAGAAATACCATTCTTATCAAAAGATGAAATTAGCGAAGGCGTTCGCTTATC
ATGTCAAGTGGTTGTCACTGAAGACATGCAAGTTGAAGTACCACCTTCCGTATTAAATGTTAAACCTTTCTATGGTAAAG
TCGTTGAAGTTGAGCAATTAACACACGATATTAGACGTATTAAATTCGAAATGAAAGAAGATTTCGTATTCAAACCAGGA
CAATACTTACAAATCGAAGTACCTGGTATTGAAACAACGAGAGCATACTCTATTGCATCAAATCCAAATGATTTAAGACA
CCCTGAGGTAATTATTCGTTTAGTACCAGGCGGTGTTGCAACTAAGTTTATTCATAAAGCTTTAGTTATAGGCGATACAT
TAAAAATTACAGGTCCATTTGGTGACTTCTTCTTACAAGAAGAGTCTACTCGCCCAATCATTATGATTGCTGGTGGTTCT
GGTAAAGCTCCAATTCGTTCAATTATCTATAAATTAATTGAACAAAACATGCCAAGAAAAGCACAATACTTCTTTGGTGC
AAGAACTAAAAAAGATTTATATTATACTAAAGAATTCAAAGATGTTGAAGCGATGTATCCAAACTTCAAATATATTCCAG
CTTTATCTACACCACTTCCAGAAGATGAGTGGGATCTAGATATCGGTCTTATTACAGAAGTTGTTGCAAGACACACTGGT
GATCTAAGTGGTCACGAAGCATATCTTTGTGGATCTCCTGGTATGATCGATGCATGTATTAAAGTATTAAGAGAAAAAGG
TATGCCTGAAGAACATATCTACTTCGATAAATTCTAA

Upstream 100 bases:

>100_bases
TTTACGTGGTCGCGCGATTGCATTCTTAATTTTAGGGATCTTAGCATTAGCATTCATTGGCTTTACCGGTATCGGTAGAG
TGTCATTTTAGGAGGAAACT

Downstream 100 bases:

>100_bases
TTTTAAAGGTGAGCTTAATGCTCACCTTTTTATTTTATGATATTCAGCTTATAAAAAAACTCACCTTATTTTGGGTGAGT
TTTATAATCATTTTTGACTA

Product: Na+-transporting NADH:ubiquinone oxidoreductase subunit F

Products: NA

Alternate protein names: Phenol 2-monooxygenase P5 component [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDPLYTPIILIGLLIVVTLVVILVDKLLGGGGERKITVNKDNVITISGRETALNALTNNKIFLPSSCGGKATCGTCKFRL
VDWHEAPKPTEIPFLSKDEISEGVRLSCQVVVTEDMQVEVPPSVLNVKPFYGKVVEVEQLTHDIRRIKFEMKEDFVFKPG
QYLQIEVPGIETTRAYSIASNPNDLRHPEVIIRLVPGGVATKFIHKALVIGDTLKITGPFGDFFLQEESTRPIIMIAGGS
GKAPIRSIIYKLIEQNMPRKAQYFFGARTKKDLYYTKEFKDVEAMYPNFKYIPALSTPLPEDEWDLDIGLITEVVARHTG
DLSGHEAYLCGSPGMIDACIKVLREKGMPEEHIYFDKF

Sequences:

>Translated_358_residues
MDPLYTPIILIGLLIVVTLVVILVDKLLGGGGERKITVNKDNVITISGRETALNALTNNKIFLPSSCGGKATCGTCKFRL
VDWHEAPKPTEIPFLSKDEISEGVRLSCQVVVTEDMQVEVPPSVLNVKPFYGKVVEVEQLTHDIRRIKFEMKEDFVFKPG
QYLQIEVPGIETTRAYSIASNPNDLRHPEVIIRLVPGGVATKFIHKALVIGDTLKITGPFGDFFLQEESTRPIIMIAGGS
GKAPIRSIIYKLIEQNMPRKAQYFFGARTKKDLYYTKEFKDVEAMYPNFKYIPALSTPLPEDEWDLDIGLITEVVARHTG
DLSGHEAYLCGSPGMIDACIKVLREKGMPEEHIYFDKF
>Mature_358_residues
MDPLYTPIILIGLLIVVTLVVILVDKLLGGGGERKITVNKDNVITISGRETALNALTNNKIFLPSSCGGKATCGTCKFRL
VDWHEAPKPTEIPFLSKDEISEGVRLSCQVVVTEDMQVEVPPSVLNVKPFYGKVVEVEQLTHDIRRIKFEMKEDFVFKPG
QYLQIEVPGIETTRAYSIASNPNDLRHPEVIIRLVPGGVATKFIHKALVIGDTLKITGPFGDFFLQEESTRPIIMIAGGS
GKAPIRSIIYKLIEQNMPRKAQYFFGARTKKDLYYTKEFKDVEAMYPNFKYIPALSTPLPEDEWDLDIGLITEVVARHTG
DLSGHEAYLCGSPGMIDACIKVLREKGMPEEHIYFDKF

Specific function: Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme [H]

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:
GO:0009055: Na+-transporting NADH:ubiquinone oxidoreductase subunit F
GO:0016491: Na+-transporting NADH:ubiquinone oxidoreductase subunit F
GO:0046872: Na+-transporting NADH:ubiquinone oxidoreductase subunit F
GO:0051536: Na+-transporting NADH:ubiquinone oxidoreductase subunit F
GO:0051537: Na+-transporting NADH:ubiquinone oxidoreductase subunit F
GO:0055114: Na+-transporting NADH:ubiquinone oxidoreductase subunit F

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=227, Percent_Identity=25.9911894273128, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787658, Length=243, Percent_Identity=25.1028806584362, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788903, Length=212, Percent_Identity=24.5283018867925, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787098, Length=158, Percent_Identity=24.6835443037975, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322146, Length=214, Percent_Identity=30.3738317757009, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323510, Length=212, Percent_Identity=24.5283018867925, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323552, Length=190, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=249, Percent_Identity=22.8915662650602, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 40019; Mature: 40019

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLYTPIILIGLLIVVTLVVILVDKLLGGGGERKITVNKDNVITISGRETALNALTNNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHCCCE
IFLPSSCGGKATCGTCKFRLVDWHEAPKPTEIPFLSKDEISEGVRLSCQVVVTEDMQVEV
EEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCEEEEC
PPSVLNVKPFYGKVVEVEQLTHDIRRIKFEMKEDFVFKPGQYLQIEVPGIETTRAYSIAS
CCCCEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCEEEEECC
NPNDLRHPEVIIRLVPGGVATKFIHKALVIGDTLKITGPFGDFFLQEESTRPIIMIAGGS
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHEEECCCCCCEEEEECCC
GKAPIRSIIYKLIEQNMPRKAQYFFGARTKKDLYYTKEFKDVEAMYPNFKYIPALSTPLP
CCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEHHHCHHHHHCCCCEECCCCCCCCC
EDEWDLDIGLITEVVARHTGDLSGHEAYLCGSPGMIDACIKVLREKGMPEEHIYFDKF
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHEEEECC
>Mature Secondary Structure
MDPLYTPIILIGLLIVVTLVVILVDKLLGGGGERKITVNKDNVITISGRETALNALTNNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHCCCE
IFLPSSCGGKATCGTCKFRLVDWHEAPKPTEIPFLSKDEISEGVRLSCQVVVTEDMQVEV
EEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCEEEEC
PPSVLNVKPFYGKVVEVEQLTHDIRRIKFEMKEDFVFKPGQYLQIEVPGIETTRAYSIAS
CCCCEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCEEEEECC
NPNDLRHPEVIIRLVPGGVATKFIHKALVIGDTLKITGPFGDFFLQEESTRPIIMIAGGS
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHEEECCCCCCEEEEECCC
GKAPIRSIIYKLIEQNMPRKAQYFFGARTKKDLYYTKEFKDVEAMYPNFKYIPALSTPLP
CCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEHHHCHHHHHCCCCEECCCCCCCCC
EDEWDLDIGLITEVVARHTGDLSGHEAYLCGSPGMIDACIKVLREKGMPEEHIYFDKF
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12732969 [H]