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The map label for this gene is mcrB [H]

Identifier: 162447493

GI number: 162447493

Start: 657505

End: 659949

Strand: Direct

Name: mcrB [H]

Synonym: ACL_0633

Alternate gene names: 162447493

Gene position: 657505-659949 (Clockwise)

Preceding gene: 162447492

Following gene: 162447494

Centisome position: 43.92

GC content: 27.89

Gene sequence:

>2445_bases
ATGATTAATGATATTAATAGGTTTATACAATTAGCAGACACTAATAATTTAAAAACAAAAGAAATAGAGACTCAAATTAC
AAATAATAATAGAGATATTATTGGCAATTTTCATGTAACTGTATCTTTTGGAGTTACAAATGTCGCTAACATACCGTGGT
TTTCTATTAGCAGATTTACTGACTATAAAGAAGATGCTCCGGTTTTTTTGTATTACAAAAAGCAAAACAAATTAGTTCTA
TCATTTGGTGTTAAAGAGGAAAAAAATAATAAGGATAGTTTGAAATATAATTTTAAATGGAATGATTTAATAAGAGAAAA
TTATCAAACTGTTCAAGAATATTTTGGTGAAAGAGTAGATAGATATGGTGACAGTTATGTGTATAAAACCTTTGATGTTG
CAAATTCAGTAGTAGCTAATGGAGAATCTATTAGCACTGATTTAACAGAAATGTTAATGACATATAAAAAACAATTACCT
TGTGGTTCACTAATTAATTATATAAAGGCTAATTATACAAGTTATCTTAATAAATTTTTAGGAGTAATGGATAAAGAAAG
AGTGGATTTTCTTAATGCATTCCCGTTAAACAATTTGTTGAATATAACCTTAGATGATTATAGTAAAAACGGGAATAAAG
ATTCATTCACAAATTATATTGAAAATAAAACTTCTAACATATGTTCGGGAAATTTAGGTATAAATCCAAACAAATTATTT
TATCCTAAGAATAATGGGTATGAAGTTTTAAGTACAGTAAGTAGTGCATATGAAACGTTCGGATCTGTTAATTTAAAGTT
TGATGAATTCAAGAGAGAATTACACTATTTTATAACGAACTTTAATATTAATAATTATCAAACACTAAATGTATTGAAAT
TTAGAGCAAATATTATAAAGTTCAATGTACTTAGACTATATAGAAATGATGTAGCTTTATATGGTTTGCCATCACAAAGA
GAATTGTCTAAGATATTAGTGAAGATAGGGCTGAACATAAATAGCGATGATAGTATTGCAAAAAGCATCATTCTTACTAA
CTATTTAATTAGTCAAGACCCACAAATTGTAAATTTGAATACGGATATTGTAAATAGATTAATTTGGGATTACAAAGTTG
ATTGTATAGATTCTAATGTTAATCAAGAAGATTCAAGCGAAGACTCTAATGAGGAGGAGAAAATTTCAATGAATAAGAAC
TTTGATAAGAACTTAATATTATATGGTCCTCCAGGGACAGGTAAAACATATAACACTAAGATATATGCTGTTGCTATTTG
CGATGATCTTGATTTAGATGAAGTAAAAAATCGAAACTATGACGATGTTTTGAAAAGATATGATGAGTTAGTGTTAGAAA
ACAGAGTTAAATTTACAACGTTCCATCAATCCTATGGTTATGAAGACTTTATTGAAGGTTTATATCCTGAAATTAAAAAT
GGCTCTGACCAAATTACTTATCGAGTTAAAGCAGGAGTTTTTAAAGCTTTTTGTGCGAATGAATCCTTTGATAGTCTTTG
GGATTCCTTTGTCTTAGATGTTAAGAATGGAAAAGTTAAATGGGGAGATATTCTCCCTAACGCTGACGAACACAAAAAAG
ATACAGTTTTAAGCGTAAATGGCAACGGAAATATTGATCTTCCTTATGCAAACGGGGTTCCATTTACAAGAGAAAATGCA
AAAAAAATCTATTTAGGTCAAGAAGTTGATAACACGCAAAAAGATTATTTTGAAAAAGTATTTAATTATTTCAAGAAAAA
TTATATGAATAAACAAAATAAGGGCAATAAAAAAGTGTTTATTATTGACGAAATTAACCGAGGCAATATTTCTAAAATAT
TTGGAGAATTAATCACCTTAATTGAAGATACAAAACGTAAAGGTGAAAAAGAAGAAATGAGTATTACATTACCATACTCT
AAAAAACCATTTACAGTTCCAAATAATGTTCACATTCTAGGTACAATGAACACTGCTGACAGATCGATTGCTTTAATGGA
TACAGCATTAAGAAGAAGATTTGCATTTGAAGAGATGATGCCAGAACCAGAACTTATTAAAGCAGAAGTTGATGGATTGA
AAATATCTGAGATGTTGACAGCAATTAACAAAAGAATTGAAGTTTTATATGATAGAGAACATACTATTGGACATGCATTT
TTTATGGACAAAAAGTTGGAACTAGGTGATCTAGCTCACATATTTAAAAATAAAATAATTCCTTTATTACAAGAGTACTT
CTATGAGGACTATGAAAAAATCCAATGGGTGCTTGGTGATAATGAAAAAACTGATGAATCATTTAAATTTATCAAACAAA
TAAAAAATGAAGCAAATATTTTCAAAGGTAAAAATGGTGCTGATATGAATGCTCTTCCAGAGTATAGATATGAGATAAAT
AAAAAGGCATTTGACAAAATAGAAAGTTACAAACAAATAAAATAA

Upstream 100 bases:

>100_bases
CATCATTAGCGTAGTTACTATGTAATGTGCTAGAATATATCTAAATGAGAACGGTAAGTAAACAAGATTTAAAAATGAAT
TAAATGAGAGGGCTTTTATA

Downstream 100 bases:

>100_bases
TAGATAGGTGGTGAACTCAGTGAAAAAGCCTCTGATTGTTAGAGAATATGATGAAATTATTAACTTAGATAATAAAAAAG
ATGGAATGATAGTTAAAACT

Product: McrBC 5-methylcytosine restriction system component

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MINDINRFIQLADTNNLKTKEIETQITNNNRDIIGNFHVTVSFGVTNVANIPWFSISRFTDYKEDAPVFLYYKKQNKLVL
SFGVKEEKNNKDSLKYNFKWNDLIRENYQTVQEYFGERVDRYGDSYVYKTFDVANSVVANGESISTDLTEMLMTYKKQLP
CGSLINYIKANYTSYLNKFLGVMDKERVDFLNAFPLNNLLNITLDDYSKNGNKDSFTNYIENKTSNICSGNLGINPNKLF
YPKNNGYEVLSTVSSAYETFGSVNLKFDEFKRELHYFITNFNINNYQTLNVLKFRANIIKFNVLRLYRNDVALYGLPSQR
ELSKILVKIGLNINSDDSIAKSIILTNYLISQDPQIVNLNTDIVNRLIWDYKVDCIDSNVNQEDSSEDSNEEEKISMNKN
FDKNLILYGPPGTGKTYNTKIYAVAICDDLDLDEVKNRNYDDVLKRYDELVLENRVKFTTFHQSYGYEDFIEGLYPEIKN
GSDQITYRVKAGVFKAFCANESFDSLWDSFVLDVKNGKVKWGDILPNADEHKKDTVLSVNGNGNIDLPYANGVPFTRENA
KKIYLGQEVDNTQKDYFEKVFNYFKKNYMNKQNKGNKKVFIIDEINRGNISKIFGELITLIEDTKRKGEKEEMSITLPYS
KKPFTVPNNVHILGTMNTADRSIALMDTALRRRFAFEEMMPEPELIKAEVDGLKISEMLTAINKRIEVLYDREHTIGHAF
FMDKKLELGDLAHIFKNKIIPLLQEYFYEDYEKIQWVLGDNEKTDESFKFIKQIKNEANIFKGKNGADMNALPEYRYEIN
KKAFDKIESYKQIK

Sequences:

>Translated_814_residues
MINDINRFIQLADTNNLKTKEIETQITNNNRDIIGNFHVTVSFGVTNVANIPWFSISRFTDYKEDAPVFLYYKKQNKLVL
SFGVKEEKNNKDSLKYNFKWNDLIRENYQTVQEYFGERVDRYGDSYVYKTFDVANSVVANGESISTDLTEMLMTYKKQLP
CGSLINYIKANYTSYLNKFLGVMDKERVDFLNAFPLNNLLNITLDDYSKNGNKDSFTNYIENKTSNICSGNLGINPNKLF
YPKNNGYEVLSTVSSAYETFGSVNLKFDEFKRELHYFITNFNINNYQTLNVLKFRANIIKFNVLRLYRNDVALYGLPSQR
ELSKILVKIGLNINSDDSIAKSIILTNYLISQDPQIVNLNTDIVNRLIWDYKVDCIDSNVNQEDSSEDSNEEEKISMNKN
FDKNLILYGPPGTGKTYNTKIYAVAICDDLDLDEVKNRNYDDVLKRYDELVLENRVKFTTFHQSYGYEDFIEGLYPEIKN
GSDQITYRVKAGVFKAFCANESFDSLWDSFVLDVKNGKVKWGDILPNADEHKKDTVLSVNGNGNIDLPYANGVPFTRENA
KKIYLGQEVDNTQKDYFEKVFNYFKKNYMNKQNKGNKKVFIIDEINRGNISKIFGELITLIEDTKRKGEKEEMSITLPYS
KKPFTVPNNVHILGTMNTADRSIALMDTALRRRFAFEEMMPEPELIKAEVDGLKISEMLTAINKRIEVLYDREHTIGHAF
FMDKKLELGDLAHIFKNKIIPLLQEYFYEDYEKIQWVLGDNEKTDESFKFIKQIKNEANIFKGKNGADMNALPEYRYEIN
KKAFDKIESYKQIK
>Mature_814_residues
MINDINRFIQLADTNNLKTKEIETQITNNNRDIIGNFHVTVSFGVTNVANIPWFSISRFTDYKEDAPVFLYYKKQNKLVL
SFGVKEEKNNKDSLKYNFKWNDLIRENYQTVQEYFGERVDRYGDSYVYKTFDVANSVVANGESISTDLTEMLMTYKKQLP
CGSLINYIKANYTSYLNKFLGVMDKERVDFLNAFPLNNLLNITLDDYSKNGNKDSFTNYIENKTSNICSGNLGINPNKLF
YPKNNGYEVLSTVSSAYETFGSVNLKFDEFKRELHYFITNFNINNYQTLNVLKFRANIIKFNVLRLYRNDVALYGLPSQR
ELSKILVKIGLNINSDDSIAKSIILTNYLISQDPQIVNLNTDIVNRLIWDYKVDCIDSNVNQEDSSEDSNEEEKISMNKN
FDKNLILYGPPGTGKTYNTKIYAVAICDDLDLDEVKNRNYDDVLKRYDELVLENRVKFTTFHQSYGYEDFIEGLYPEIKN
GSDQITYRVKAGVFKAFCANESFDSLWDSFVLDVKNGKVKWGDILPNADEHKKDTVLSVNGNGNIDLPYANGVPFTRENA
KKIYLGQEVDNTQKDYFEKVFNYFKKNYMNKQNKGNKKVFIIDEINRGNISKIFGELITLIEDTKRKGEKEEMSITLPYS
KKPFTVPNNVHILGTMNTADRSIALMDTALRRRFAFEEMMPEPELIKAEVDGLKISEMLTAINKRIEVLYDREHTIGHAF
FMDKKLELGDLAHIFKNKIIPLLQEYFYEDYEKIQWVLGDNEKTDESFKFIKQIKNEANIFKGKNGADMNALPEYRYEIN
KKAFDKIESYKQIK

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:
GO:0000166: McrBC 5-methylcytosine restriction system component
GO:0005524: McrBC 5-methylcytosine restriction system component
GO:0006200: McrBC 5-methylcytosine restriction system component
GO:0016787: McrBC 5-methylcytosine restriction system component
GO:0016887: McrBC 5-methylcytosine restriction system component
GO:0017111: McrBC 5-methylcytosine restriction system component

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=200, Percent_Identity=39, Blast_Score=113, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 94542; Mature: 94542

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDINRFIQLADTNNLKTKEIETQITNNNRDIIGNFHVTVSFGVTNVANIPWFSISRFT
CCCCHHHHEEEECCCCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCEEEHHCC
DYKEDAPVFLYYKKQNKLVLSFGVKEEKNNKDSLKYNFKWNDLIRENYQTVQEYFGERVD
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
RYGDSYVYKTFDVANSVVANGESISTDLTEMLMTYKKQLPCGSLINYIKANYTSYLNKFL
HCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GVMDKERVDFLNAFPLNNLLNITLDDYSKNGNKDSFTNYIENKTSNICSGNLGINPNKLF
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEE
YPKNNGYEVLSTVSSAYETFGSVNLKFDEFKRELHYFITNFNINNYQTLNVLKFRANIIK
ECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEEECCCCCEEEEHEEEEHHHHHH
FNVLRLYRNDVALYGLPSQRELSKILVKIGLNINSDDSIAKSIILTNYLISQDPQIVNLN
HHEEEHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
TDIVNRLIWDYKVDCIDSNVNQEDSSEDSNEEEKISMNKNFDKNLILYGPPGTGKTYNTK
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCCCEE
IYAVAICDDLDLDEVKNRNYDDVLKRYDELVLENRVKFTTFHQSYGYEDFIEGLYPEIKN
EEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHCCHHHCC
GSDQITYRVKAGVFKAFCANESFDSLWDSFVLDVKNGKVKWGDILPNADEHKKDTVLSVN
CCCEEEEEEEHHHHHHHHCCCCHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCEEEEC
GNGNIDLPYANGVPFTRENAKKIYLGQEVDNTQKDYFEKVFNYFKKNYMNKQNKGNKKVF
CCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IIDEINRGNISKIFGELITLIEDTKRKGEKEEMSITLPYSKKPFTVPNNVHILGTMNTAD
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC
RSIALMDTALRRRFAFEEMMPEPELIKAEVDGLKISEMLTAINKRIEVLYDREHTIGHAF
CHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCEEHHHHHHHHHHHHEEEECCCCCCEEEE
FMDKKLELGDLAHIFKNKIIPLLQEYFYEDYEKIQWVLGDNEKTDESFKFIKQIKNEANI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHE
FKGKNGADMNALPEYRYEINKKAFDKIESYKQIK
ECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure
MINDINRFIQLADTNNLKTKEIETQITNNNRDIIGNFHVTVSFGVTNVANIPWFSISRFT
CCCCHHHHEEEECCCCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCEEEHHCC
DYKEDAPVFLYYKKQNKLVLSFGVKEEKNNKDSLKYNFKWNDLIRENYQTVQEYFGERVD
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
RYGDSYVYKTFDVANSVVANGESISTDLTEMLMTYKKQLPCGSLINYIKANYTSYLNKFL
HCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GVMDKERVDFLNAFPLNNLLNITLDDYSKNGNKDSFTNYIENKTSNICSGNLGINPNKLF
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEE
YPKNNGYEVLSTVSSAYETFGSVNLKFDEFKRELHYFITNFNINNYQTLNVLKFRANIIK
ECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEEECCCCCEEEEHEEEEHHHHHH
FNVLRLYRNDVALYGLPSQRELSKILVKIGLNINSDDSIAKSIILTNYLISQDPQIVNLN
HHEEEHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
TDIVNRLIWDYKVDCIDSNVNQEDSSEDSNEEEKISMNKNFDKNLILYGPPGTGKTYNTK
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCCCEE
IYAVAICDDLDLDEVKNRNYDDVLKRYDELVLENRVKFTTFHQSYGYEDFIEGLYPEIKN
EEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHCCHHHCC
GSDQITYRVKAGVFKAFCANESFDSLWDSFVLDVKNGKVKWGDILPNADEHKKDTVLSVN
CCCEEEEEEEHHHHHHHHCCCCHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCEEEEC
GNGNIDLPYANGVPFTRENAKKIYLGQEVDNTQKDYFEKVFNYFKKNYMNKQNKGNKKVF
CCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IIDEINRGNISKIFGELITLIEDTKRKGEKEEMSITLPYSKKPFTVPNNVHILGTMNTAD
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC
RSIALMDTALRRRFAFEEMMPEPELIKAEVDGLKISEMLTAINKRIEVLYDREHTIGHAF
CHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCEEHHHHHHHHHHHHEEEECCCCCCEEEE
FMDKKLELGDLAHIFKNKIIPLLQEYFYEDYEKIQWVLGDNEKTDESFKFIKQIKNEANI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHE
FKGKNGADMNALPEYRYEINKKAFDKIESYKQIK
ECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]