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The map label for this gene is metK2 [H]

Identifier: 162447461

GI number: 162447461

Start: 631894

End: 633042

Strand: Direct

Name: metK2 [H]

Synonym: ACL_0600

Alternate gene names: 162447461

Gene position: 631894-633042 (Clockwise)

Preceding gene: 162447460

Following gene: 162447462

Centisome position: 42.21

GC content: 38.82

Gene sequence:

>1149_bases
ATGAAAATTATAACAAGTGAATCTGTCTTTAAAGGACACCCAGATAAAATATGTGACCAAATCAGTGATGCAATCTTAGA
TGCACATTTAGAGCAAGACAGAAATGCAAGAGTAGCAGTAGAAACAGCTATCAAAGATGATGTTGTTTTTATCTTTGGTG
AGGTCACATCCAAAGCTTCAGTGAATTATAAAGAAGTCGCACTAACCACATTAAAAGAAATCGGATATGATGATCCATTC
GATGTGATTGAAAAGATATCCAAACAGTCCGATGATATTGCACTTGGTGTAAATCATACGAACGATCATGAACAAGGTGC
AGGTGATCAAGGCTTAATGTTTGGCTACGCTTGCAAAGAAACGCCAGAATTTATGCCTTTACCGATTGTGGTGGCACATG
ACATTGCAAGAAGCATTGACGAGCTTAGAAAGGCAAAGTATGGCCACGTGTTTGGTCCAGATGGTAAGTGTCAAGTTTCT
GTAGTCTACGAGAATGATGAACCGATTGCTTATGAAACAATCGTAGTGTCTGCGCAAACAAAGCATGGCATTCAATTAGA
TTATGCTAAAGAGATAATTTTAAATGAAGTATTGAAACTACTGATAGGTGAAGACCTTTCACACATTCAAGTCTTAATCA
ATCCAACAGGTGCTTTTGTCGTTGGTGGACCATATGGAGATTCTGGATTAACTGGTAGAAAGATTATTGTTGATACCTAT
GGTGGTTATGCTAAACATGGCGGTGGCGCATTCTCTGGCAAGGATGTGAGCAAGGTAGACCGCAGTGCGGCTTACTATGC
CAGATTCGTAGCAAAAGCCCTTGTAGCGGCAAATTTAGCGAACAGGTGCGAAGTTAGTGTGTCCTATTCCATCGGTGTAG
CAAACCCTGTAAGTGTATCCGTTGATACCTTTGGAACAGGTGTTACTTCTGATGATGTTTTATTAAAGTTAATTAAAGAG
AACTTCAACTTTACACCGTCAAGTATTATCAAAGAACTAAACCTGGACAAAGTGAAGTTTAAACCGTTAGCATCTTATGG
ACATATAGGTCGTGTTGATCTTGATGTTGCATGGGAAAAAGTAGATGCAAAAGCTAAACAATTAAGTGAATCTTATGAGC
AAACCAAAAGAACTGCACAGGTTCTATAA

Upstream 100 bases:

>100_bases
ACGCTAGGTATGCAGCATTCATACCACTTATTAAAACCAAAACAGAAAATTAAGGAAGCGAGTAAGCTTCCTTTTTAACT
ATTTAAAGCGAGGAAAACAA

Downstream 100 bases:

>100_bases
GTCGAAGGCTTGGCTCGTTGCTCGTAATATCAAAACAAACGCCACACAGGGCAAGTGTGAGCGGTGTGGCGCGATTGGTG
AAGAGGTTCACCATAAGATT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKIITSESVFKGHPDKICDQISDAILDAHLEQDRNARVAVETAIKDDVVFIFGEVTSKASVNYKEVALTTLKEIGYDDPF
DVIEKISKQSDDIALGVNHTNDHEQGAGDQGLMFGYACKETPEFMPLPIVVAHDIARSIDELRKAKYGHVFGPDGKCQVS
VVYENDEPIAYETIVVSAQTKHGIQLDYAKEIILNEVLKLLIGEDLSHIQVLINPTGAFVVGGPYGDSGLTGRKIIVDTY
GGYAKHGGGAFSGKDVSKVDRSAAYYARFVAKALVAANLANRCEVSVSYSIGVANPVSVSVDTFGTGVTSDDVLLKLIKE
NFNFTPSSIIKELNLDKVKFKPLASYGHIGRVDLDVAWEKVDAKAKQLSESYEQTKRTAQVL

Sequences:

>Translated_382_residues
MKIITSESVFKGHPDKICDQISDAILDAHLEQDRNARVAVETAIKDDVVFIFGEVTSKASVNYKEVALTTLKEIGYDDPF
DVIEKISKQSDDIALGVNHTNDHEQGAGDQGLMFGYACKETPEFMPLPIVVAHDIARSIDELRKAKYGHVFGPDGKCQVS
VVYENDEPIAYETIVVSAQTKHGIQLDYAKEIILNEVLKLLIGEDLSHIQVLINPTGAFVVGGPYGDSGLTGRKIIVDTY
GGYAKHGGGAFSGKDVSKVDRSAAYYARFVAKALVAANLANRCEVSVSYSIGVANPVSVSVDTFGTGVTSDDVLLKLIKE
NFNFTPSSIIKELNLDKVKFKPLASYGHIGRVDLDVAWEKVDAKAKQLSESYEQTKRTAQVL
>Mature_382_residues
MKIITSESVFKGHPDKICDQISDAILDAHLEQDRNARVAVETAIKDDVVFIFGEVTSKASVNYKEVALTTLKEIGYDDPF
DVIEKISKQSDDIALGVNHTNDHEQGAGDQGLMFGYACKETPEFMPLPIVVAHDIARSIDELRKAKYGHVFGPDGKCQVS
VVYENDEPIAYETIVVSAQTKHGIQLDYAKEIILNEVLKLLIGEDLSHIQVLINPTGAFVVGGPYGDSGLTGRKIIVDTY
GGYAKHGGGAFSGKDVSKVDRSAAYYARFVAKALVAANLANRCEVSVSYSIGVANPVSVSVDTFGTGVTSDDVLLKLIKE
NFNFTPSSIIKELNLDKVKFKPLASYGHIGRVDLDVAWEKVDAKAKQLSESYEQTKRTAQVL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:
GO:0000166: S-adenosylmethionine synthase
GO:0004478: S-adenosylmethionine synthase
GO:0005524: S-adenosylmethionine synthase
GO:0005737: S-adenosylmethionine synthase
GO:0006556: S-adenosylmethionine synthase
GO:0006730: S-adenosylmethionine synthase
GO:0016740: S-adenosylmethionine synthase
GO:0046872: S-adenosylmethionine synthase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=49.2021276595745, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=48.1382978723404, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1789311, Length=379, Percent_Identity=49.868073878628, Blast_Score=347, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=47.9892761394102, Blast_Score=317, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=47.9892761394102, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI25145633, Length=373, Percent_Identity=48.2573726541555, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17551082, Length=376, Percent_Identity=47.6063829787234, Blast_Score=307, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI32565905, Length=365, Percent_Identity=44.3835616438356, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=45.4268292682927, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI25145635, Length=328, Percent_Identity=45.7317073170732, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI32565903, Length=287, Percent_Identity=46.3414634146341, Blast_Score=230, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI32565909, Length=76, Percent_Identity=57.8947368421053, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=373, Percent_Identity=48.5254691689008, Blast_Score=325, Evalue=7e-90,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=48.2573726541555, Blast_Score=322, Evalue=9e-89,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=50, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=49.4652406417112, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=49.4652406417112, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24580541, Length=362, Percent_Identity=48.8950276243094, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=52.5423728813559, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41593; Mature: 41593

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITSESVFKGHPDKICDQISDAILDAHLEQDRNARVAVETAIKDDVVFIFGEVTSKAS
CEEECCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCC
VNYKEVALTTLKEIGYDDPFDVIEKISKQSDDIALGVNHTNDHEQGAGDQGLMFGYACKE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCC
TPEFMPLPIVVAHDIARSIDELRKAKYGHVFGPDGKCQVSVVYENDEPIAYETIVVSAQT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCEEEEEEEEEECC
KHGIQLDYAKEIILNEVLKLLIGEDLSHIQVLINPTGAFVVGGPYGDSGLTGRKIIVDTY
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEECC
GGYAKHGGGAFSGKDVSKVDRSAAYYARFVAKALVAANLANRCEVSVSYSIGVANPVSVS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCEEE
VDTFGTGVTSDDVLLKLIKENFNFTPSSIIKELNLDKVKFKPLASYGHIGRVDLDVAWEK
EECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHH
VDAKAKQLSESYEQTKRTAQVL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIITSESVFKGHPDKICDQISDAILDAHLEQDRNARVAVETAIKDDVVFIFGEVTSKAS
CEEECCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCC
VNYKEVALTTLKEIGYDDPFDVIEKISKQSDDIALGVNHTNDHEQGAGDQGLMFGYACKE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCC
TPEFMPLPIVVAHDIARSIDELRKAKYGHVFGPDGKCQVSVVYENDEPIAYETIVVSAQT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCEEEEEEEEEECC
KHGIQLDYAKEIILNEVLKLLIGEDLSHIQVLINPTGAFVVGGPYGDSGLTGRKIIVDTY
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEECC
GGYAKHGGGAFSGKDVSKVDRSAAYYARFVAKALVAANLANRCEVSVSYSIGVANPVSVS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCEEE
VDTFGTGVTSDDVLLKLIKENFNFTPSSIIKELNLDKVKFKPLASYGHIGRVDLDVAWEK
EECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHH
VDAKAKQLSESYEQTKRTAQVL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA