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The map label for this gene is yefA [H]

Identifier: 162447431

GI number: 162447431

Start: 604495

End: 605637

Strand: Direct

Name: yefA [H]

Synonym: ACL_0569

Alternate gene names: 162447431

Gene position: 604495-605637 (Clockwise)

Preceding gene: 162447430

Following gene: 162447432

Centisome position: 40.38

GC content: 28.78

Gene sequence:

>1143_bases
ATGAGTAAAGATTATAATGATGATATAGCATTTGATACATTAGATACGACAGCTTTTGAACAAATGCCTTATAAAGAACA
ACTGCAAGTAAAAGAAAAATACATAAAATCTCTTTTTAATAGAGAATTTTTACCAATAGTACCTAATCCTGTACCTAGAG
GTTATAGACATAAAGCGGTGTTATCTGCAACCAATGTCAATGTGAATAATAACCAACAAATACGTTTAGGTTTATTTATT
GAAGGCACTACAAAAATTAAACCTAAACTAGGTCATTTTTTACATGATAAAGAAATAGATTTAGTATTTGAAACAGTTGA
AAAACTTCTTATTAAATATAAACATAAAGCCTATGCTAAAAATTACCGACAAGGAATTATTAAGCATGTTATGATTAGAA
AATCATTTAAAACAGGTGATTTAATGGTTATCTTTTCTACCCAATTAAATAATTTTCCAAATCATAAAAAGATTGTAAAT
GAGCTGATTGCACTACACCCAAATGTAAAAACTGTGATTCAAAATATCCATAAAAAAGACTCTAAGTTTGTCTTACTAGA
AGAACAACGTATTTTATATGGTAAAGGCTTTATCATCGATAGTATCGGTGATATAGATTTTAAGATATCTGCTAATGCAT
TCTATCAAATTAATCCGATGCAAATGTTCAATTTATATGATAAGGTTTTTGAGTTTGCAGGCATTGAAAAAACAGATGTA
GTCATAGATGCCTATTCTGGAATTGGTACGATCACACTACTAGCATCTAAATATGCTAAAAAAGTATATGGTTTAGAAAT
TAACCCCGCATCTCATAAAGATGCTATTGATAACAAACGTATGAATCAAATTACTAACGCAGAATTTATTTTAGGTGATG
TGGAACACACACTTGAAAACTTTACTGACAAGGTAGATACATTAATTATGGACCCAGCAAGAGATGGTGCAAGTCTTAAA
TTTATCCAAACAGTATTAAAACTAAAACCTAAAAAAATAGTGTATGTATCATGTAATCCGGATACTCAAGAAAGAGATTA
TAAGCAATTAAGAAACTTTTATAATCTAACCAAAATATTACCATTTGATATGTTTAGTTACACACCACACGTTGAAAACA
TCGTATTGCTCGAATTAAAATAG

Upstream 100 bases:

>100_bases
CTGGTTGGTACAACTATGATCAAGTATTAAAATACTTAACATATGATAATGATAAAATCGCCTTTATTAAACTGACCAAA
GAATTAAATAAGGATATAAA

Downstream 100 bases:

>100_bases
CCTTATAAAGCCATCAAGTAGACAATCAAGAAGCCGTATTCCAGCGAATACGGCTCATTTTTTGAAATTTATACGAAAAC
ATATCAAAAATATGTTACTT

Product: TrmA family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSKDYNDDIAFDTLDTTAFEQMPYKEQLQVKEKYIKSLFNREFLPIVPNPVPRGYRHKAVLSATNVNVNNNQQIRLGLFI
EGTTKIKPKLGHFLHDKEIDLVFETVEKLLIKYKHKAYAKNYRQGIIKHVMIRKSFKTGDLMVIFSTQLNNFPNHKKIVN
ELIALHPNVKTVIQNIHKKDSKFVLLEEQRILYGKGFIIDSIGDIDFKISANAFYQINPMQMFNLYDKVFEFAGIEKTDV
VIDAYSGIGTITLLASKYAKKVYGLEINPASHKDAIDNKRMNQITNAEFILGDVEHTLENFTDKVDTLIMDPARDGASLK
FIQTVLKLKPKKIVYVSCNPDTQERDYKQLRNFYNLTKILPFDMFSYTPHVENIVLLELK

Sequences:

>Translated_380_residues
MSKDYNDDIAFDTLDTTAFEQMPYKEQLQVKEKYIKSLFNREFLPIVPNPVPRGYRHKAVLSATNVNVNNNQQIRLGLFI
EGTTKIKPKLGHFLHDKEIDLVFETVEKLLIKYKHKAYAKNYRQGIIKHVMIRKSFKTGDLMVIFSTQLNNFPNHKKIVN
ELIALHPNVKTVIQNIHKKDSKFVLLEEQRILYGKGFIIDSIGDIDFKISANAFYQINPMQMFNLYDKVFEFAGIEKTDV
VIDAYSGIGTITLLASKYAKKVYGLEINPASHKDAIDNKRMNQITNAEFILGDVEHTLENFTDKVDTLIMDPARDGASLK
FIQTVLKLKPKKIVYVSCNPDTQERDYKQLRNFYNLTKILPFDMFSYTPHVENIVLLELK
>Mature_379_residues
SKDYNDDIAFDTLDTTAFEQMPYKEQLQVKEKYIKSLFNREFLPIVPNPVPRGYRHKAVLSATNVNVNNNQQIRLGLFIE
GTTKIKPKLGHFLHDKEIDLVFETVEKLLIKYKHKAYAKNYRQGIIKHVMIRKSFKTGDLMVIFSTQLNNFPNHKKIVNE
LIALHPNVKTVIQNIHKKDSKFVLLEEQRILYGKGFIIDSIGDIDFKISANAFYQINPMQMFNLYDKVFEFAGIEKTDVV
IDAYSGIGTITLLASKYAKKVYGLEINPASHKDAIDNKRMNQITNAEFILGDVEHTLENFTDKVDTLIMDPARDGASLKF
IQTVLKLKPKKIVYVSCNPDTQERDYKQLRNFYNLTKILPFDMFSYTPHVENIVLLELK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=420, Percent_Identity=24.2857142857143, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI51173878, Length=420, Percent_Identity=24.2857142857143, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI269784680, Length=426, Percent_Identity=23.0046948356808, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI269784676, Length=426, Percent_Identity=23.0046948356808, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI20149688, Length=426, Percent_Identity=23.0046948356808, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI269784678, Length=247, Percent_Identity=25.1012145748988, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789148, Length=334, Percent_Identity=27.2455089820359, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787083, Length=362, Percent_Identity=22.0994475138122, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=374, Percent_Identity=26.2032085561497, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 43981; Mature: 43849

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDYNDDIAFDTLDTTAFEQMPYKEQLQVKEKYIKSLFNREFLPIVPNPVPRGYRHKAV
CCCCCCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LSATNVNVNNNQQIRLGLFIEGTTKIKPKLGHFLHDKEIDLVFETVEKLLIKYKHKAYAK
EEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NYRQGIIKHVMIRKSFKTGDLMVIFSTQLNNFPNHKKIVNELIALHPNVKTVIQNIHKKD
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
SKFVLLEEQRILYGKGFIIDSIGDIDFKISANAFYQINPMQMFNLYDKVFEFAGIEKTDV
CCEEEEECCEEEEECCEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCEE
VIDAYSGIGTITLLASKYAKKVYGLEINPASHKDAIDNKRMNQITNAEFILGDVEHTLEN
EEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHH
FTDKVDTLIMDPARDGASLKFIQTVLKLKPKKIVYVSCNPDTQERDYKQLRNFYNLTKIL
HHHHHHHEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHC
PFDMFSYTPHVENIVLLELK
CHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SKDYNDDIAFDTLDTTAFEQMPYKEQLQVKEKYIKSLFNREFLPIVPNPVPRGYRHKAV
CCCCCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LSATNVNVNNNQQIRLGLFIEGTTKIKPKLGHFLHDKEIDLVFETVEKLLIKYKHKAYAK
EEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NYRQGIIKHVMIRKSFKTGDLMVIFSTQLNNFPNHKKIVNELIALHPNVKTVIQNIHKKD
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
SKFVLLEEQRILYGKGFIIDSIGDIDFKISANAFYQINPMQMFNLYDKVFEFAGIEKTDV
CCEEEEECCEEEEECCEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHHHCCCCCCEE
VIDAYSGIGTITLLASKYAKKVYGLEINPASHKDAIDNKRMNQITNAEFILGDVEHTLEN
EEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHH
FTDKVDTLIMDPARDGASLKFIQTVLKLKPKKIVYVSCNPDTQERDYKQLRNFYNLTKIL
HHHHHHHEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHC
PFDMFSYTPHVENIVLLELK
CHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]