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The map label for this gene is lplA1 [H]

Identifier: 162447414

GI number: 162447414

Start: 588709

End: 589707

Strand: Direct

Name: lplA1 [H]

Synonym: ACL_0552

Alternate gene names: 162447414

Gene position: 588709-589707 (Clockwise)

Preceding gene: 162447413

Following gene: 162447415

Centisome position: 39.33

GC content: 30.23

Gene sequence:

>999_bases
ATGATACTTATAAAACATCATAATGAAGGTAATTTAGAACCATACTTTTATTTTGCATTAGAAAAATATGTATTAGAACA
TTTACTTAAAGACAATGAAACCTATTTCTTCACCTGGAGAATAAAAGGCATTGTTTCAGGTAAGAACCAAGTCATTGAAA
ATGAAATTAATATTCCCTATGTCAAAGAACATAATGTGAAATTCTTTAGAAGACCCACTGGTGGCGGATCTGTCTATGCA
GATGAAAATAACACCATGTTTACAATTATTACCAAAAGAAGTGGACAAAACTTCTCATTTAAAAACTACTTAGTCAAAAT
CACTGATGCATTTAAAAAACTAGGTGTTAATCTAGATTTTAGTGGTAGAAATGATATCTTATTAGATGGTAAAAAGGTAT
CTGGTAATGCATTCTTACAAAACAAGTATGGTATGTTAATGCATGGAACTATGATGTATGATTTAGATATTGAAACGATG
GTGCGTGCATTAAATCCACCGGATGAGAAACTTATTTCTAAAGGTATTGCAAGTGTAAGAAGTCGTGTAAACAATCTAAA
ACCTTATTTAAATGGAATGAGTCATGATGAATTAGTTAGTTATCTTGAAACAGAACTAACAAGCAGTGTTTATGAAATCA
ATCAAGAAGAAGTAGAACAACTTAAAAAAGATGCTAAATTTTATGCAAGTTCAGAATGGCTATACCGTTCTCAACCAGAA
TATACTCAAAAGCTATCTAAACGATTTGATTGGGGACATATCGATATGCGTTTTAACATCAAATCAGGTATGATTCAAAA
CGTAGATATCACTGGAGATTTCTTTGAGCTCAAAGAAGATTTAAAATCCCTTGAAAAACCATTATTAAACCAAGTTTGGA
GCTATGCATCCATTAAAGAAATTTTAAACAGTATCGAAGTAGATAAGATCATTTTAAACGCTACAAATGAAGATATCTTA
GCACTCTTTGATAATGCTTTTATAGAGACAAATAACTAA

Upstream 100 bases:

>100_bases
AGAAAGAAATCTGGTTGGGATAAATTCAAATCATTCTTTACAAATTAAGAGGATTTTTCCTCTTTTTGTTTTATAATGGA
TAAAGGAAGGAAGTGGCAAT

Downstream 100 bases:

>100_bases
AAGATAGTTCATAGGTCAATTGGTTATAACATAATTACAATTTTTGTGATAAAATGGCTCAAACTAATTGACTTATGAAG
GGTTATTTAATATAATTAAC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPYVKEHNVKFFRRPTGGGSVYA
DENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDFSGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETM
VRALNPPDEKLISKGIASVRSRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKEILNSIEVDKIILNATNEDIL
ALFDNAFIETNN

Sequences:

>Translated_332_residues
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPYVKEHNVKFFRRPTGGGSVYA
DENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDFSGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETM
VRALNPPDEKLISKGIASVRSRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKEILNSIEVDKIILNATNEDIL
ALFDNAFIETNN
>Mature_332_residues
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPYVKEHNVKFFRRPTGGGSVYA
DENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDFSGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETM
VRALNPPDEKLISKGIASVRSRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKEILNSIEVDKIILNATNEDIL
ALFDNAFIETNN

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21729884, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21729882, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21729880, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21729878, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1790846, Length=275, Percent_Identity=30.1818181818182, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322415, Length=303, Percent_Identity=24.7524752475248, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38562; Mature: 38562

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPY
CEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECCCCCCCE
VKEHNVKFFRRPTGGGSVYADENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDF
EECCCCEEEECCCCCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEE
SGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETMVRALNPPDEKLISKGIASVR
CCCCCEEEECCEECCCEEEECCCCEEEECEEEEECCHHHHHHHCCCCHHHHHHHHHHHHH
SRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
HHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCH
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKE
HHHHHHHCCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILNSIEVDKIILNATNEDILALFDNAFIETNN
HHHHHCCCEEEEECCCCHHEEEECCCEEECCC
>Mature Secondary Structure
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPY
CEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECCCCCCCE
VKEHNVKFFRRPTGGGSVYADENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDF
EECCCCEEEECCCCCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEE
SGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETMVRALNPPDEKLISKGIASVR
CCCCCEEEECCEECCCEEEECCCCEEEECEEEEECCHHHHHHHCCCCHHHHHHHHHHHHH
SRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
HHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCH
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKE
HHHHHHHCCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILNSIEVDKIILNATNEDILALFDNAFIETNN
HHHHHCCCEEEEECCCCHHEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]