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The map label for this gene is ygcA [C]

Identifier: 162447375

GI number: 162447375

Start: 537193

End: 538575

Strand: Direct

Name: ygcA [C]

Synonym: ACL_0513

Alternate gene names: 162447375

Gene position: 537193-538575 (Clockwise)

Preceding gene: 162447374

Following gene: 162447377

Centisome position: 35.88

GC content: 31.16

Gene sequence:

>1383_bases
ATGATAAACGAACAAATCAAAATAAATGATCTTATAGAAATAGAAATTAAAAAACTAGGTATTAATGGCGAAGGTATTGG
ATATTACCATAAACTTGCTGTTTTTGTTGATAATGCATTACCTGGTGAAATAATTACAGCAAAGGTGACTGAAATCTTTC
CTAACCGCTTATTAGCAGATATTGAAGAAATTAAAGTTAAAAGTCCAGATAGACTAGAAGTGATGTTTCCAGAGTATGAA
CTATGTGGTGCATACAGTATGCAACATGTGACATATGAAAAGTCACTACAAATTAAACGTGATATTTTAATTAACGCCCT
TAATAGATACGTAGATAAGAAGATAGTTTATTCAAAAATCAAACAAACAATAGGTATGAATGAACCACTTGGTTATAGAA
ACAAAGTGTCTTTACCCGTTAGAAAATTAGAAGGTAAAAACAAATTTGGATTATATGCTAAAGGTGGTAATGAATTTATA
CCAGTAAATGATACACCTGTACATCAACCTAGAATTAATGAGATTATCCAAATTGTTGAAACACTCATGGATCAACATAA
ATTTCATGCCTATATCCAAAAAGATAAAAGTGGTTATATGAAGTCTATGGTTATTAGACGATCATATACGACTGGTGAAG
TTCAAGTATCATTCTTACTCATGAAAAAATTTGAGGGTATTGCTAAGTTTACTGAAGCGCTTGTTGCACAATGTCCAGAC
ATTGTCAGTGTATTTGCATTCTATACGGATAAATATAAAGAGCAGATATTCTTTACTAACAACTACGAAAACCTATTTGG
TAAAGCAACCATTAATGAGAAAATAAATCATCAAACATTCTCATTATATCCTGAAGCCTTTTTCCAATTAAATACGGTGA
TGGCAGATAAGTTCTATAATAAAATGAGAGAACTAGCAAATCTACAACCTACAGATGTTGTCATAGACGCTTATGCAGGA
TCAGCACCAATTAGCCATTACATTGCTAAAGATGTGAAAAAAGTTTATGCAATAGAAATTGATCAAAGAAGTGTTCAAAG
TGCTATATTATCTTTAAAGCGAAATAACATTAAAAATGTGACAGTCATTCAATCTGACTTTAAGAAGGCCTTAAAAAATC
TTGAAGTGGATACGATTGATGCAATGTTCTTTGACCCACCTAGAACTGGTCTTGGCTATGATACAATTAAGCAAATACTG
AAATACAAGCCGAAGAAACTTATTTATGGTTCTTGTAATCCTTCGACTTTAGCTAAAGATTTAGAAGATTTATTAAAGAC
TTATGAACTTAAGAGTTTAATTCCAATGGATATGTTTCCTTATACCCCCTTGGTCGAAAGTGTTTCATTACTAGAATTAA
GAACAGATTTAGAGACTGTATAA

Upstream 100 bases:

>100_bases
ACACCAAACAGTCCCTTTTTTCATGAAAAAACACTAAAAAACTGTTACAATATAATTGAACTAATGATTGAAAATCATCC
TTAGTTTTTAAGGAGCACGT

Downstream 100 bases:

>100_bases
AATATATAATTATAACGAAACAGATACATAAAAGCAAAAATGCTTTATGTATCTGTTTTGTTATAGGTGATAAAAAGATA
ATTATATTATGGCTATATTG

Product: TrmA family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MINEQIKINDLIEIEIKKLGINGEGIGYYHKLAVFVDNALPGEIITAKVTEIFPNRLLADIEEIKVKSPDRLEVMFPEYE
LCGAYSMQHVTYEKSLQIKRDILINALNRYVDKKIVYSKIKQTIGMNEPLGYRNKVSLPVRKLEGKNKFGLYAKGGNEFI
PVNDTPVHQPRINEIIQIVETLMDQHKFHAYIQKDKSGYMKSMVIRRSYTTGEVQVSFLLMKKFEGIAKFTEALVAQCPD
IVSVFAFYTDKYKEQIFFTNNYENLFGKATINEKINHQTFSLYPEAFFQLNTVMADKFYNKMRELANLQPTDVVIDAYAG
SAPISHYIAKDVKKVYAIEIDQRSVQSAILSLKRNNIKNVTVIQSDFKKALKNLEVDTIDAMFFDPPRTGLGYDTIKQIL
KYKPKKLIYGSCNPSTLAKDLEDLLKTYELKSLIPMDMFPYTPLVESVSLLELRTDLETV

Sequences:

>Translated_460_residues
MINEQIKINDLIEIEIKKLGINGEGIGYYHKLAVFVDNALPGEIITAKVTEIFPNRLLADIEEIKVKSPDRLEVMFPEYE
LCGAYSMQHVTYEKSLQIKRDILINALNRYVDKKIVYSKIKQTIGMNEPLGYRNKVSLPVRKLEGKNKFGLYAKGGNEFI
PVNDTPVHQPRINEIIQIVETLMDQHKFHAYIQKDKSGYMKSMVIRRSYTTGEVQVSFLLMKKFEGIAKFTEALVAQCPD
IVSVFAFYTDKYKEQIFFTNNYENLFGKATINEKINHQTFSLYPEAFFQLNTVMADKFYNKMRELANLQPTDVVIDAYAG
SAPISHYIAKDVKKVYAIEIDQRSVQSAILSLKRNNIKNVTVIQSDFKKALKNLEVDTIDAMFFDPPRTGLGYDTIKQIL
KYKPKKLIYGSCNPSTLAKDLEDLLKTYELKSLIPMDMFPYTPLVESVSLLELRTDLETV
>Mature_460_residues
MINEQIKINDLIEIEIKKLGINGEGIGYYHKLAVFVDNALPGEIITAKVTEIFPNRLLADIEEIKVKSPDRLEVMFPEYE
LCGAYSMQHVTYEKSLQIKRDILINALNRYVDKKIVYSKIKQTIGMNEPLGYRNKVSLPVRKLEGKNKFGLYAKGGNEFI
PVNDTPVHQPRINEIIQIVETLMDQHKFHAYIQKDKSGYMKSMVIRRSYTTGEVQVSFLLMKKFEGIAKFTEALVAQCPD
IVSVFAFYTDKYKEQIFFTNNYENLFGKATINEKINHQTFSLYPEAFFQLNTVMADKFYNKMRELANLQPTDVVIDAYAG
SAPISHYIAKDVKKVYAIEIDQRSVQSAILSLKRNNIKNVTVIQSDFKKALKNLEVDTIDAMFFDPPRTGLGYDTIKQIL
KYKPKKLIYGSCNPSTLAKDLEDLLKTYELKSLIPMDMFPYTPLVESVSLLELRTDLETV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=350, Percent_Identity=23.7142857142857, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI51173878, Length=350, Percent_Identity=23.7142857142857, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI269784678, Length=361, Percent_Identity=23.8227146814404, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI269784680, Length=361, Percent_Identity=23.8227146814404, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI269784676, Length=361, Percent_Identity=23.8227146814404, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI20149688, Length=361, Percent_Identity=23.8227146814404, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789148, Length=453, Percent_Identity=23.6203090507726, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790403, Length=155, Percent_Identity=34.1935483870968, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=22.1538461538462, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24666579, Length=420, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52811; Mature: 52811

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEQIKINDLIEIEIKKLGINGEGIGYYHKLAVFVDNALPGEIITAKVTEIFPNRLLAD
CCCCCEEECCEEEEEEEEECCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
IEEIKVKSPDRLEVMFPEYELCGAYSMQHVTYEKSLQIKRDILINALNRYVDKKIVYSKI
HHHHCCCCCCCEEEECCCHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQTIGMNEPLGYRNKVSLPVRKLEGKNKFGLYAKGGNEFIPVNDTPVHQPRINEIIQIVE
HHHHCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHH
TLMDQHKFHAYIQKDKSGYMKSMVIRRSYTTGEVQVSFLLMKKFEGIAKFTEALVAQCPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCHH
IVSVFAFYTDKYKEQIFFTNNYENLFGKATINEKINHQTFSLYPEAFFQLNTVMADKFYN
HHHHHHHHHHHHHCEEEEECCHHHHCCCEEHHHCCCCCCEEECHHHHHHHHHHHHHHHHH
KMRELANLQPTDVVIDAYAGSAPISHYIAKDVKKVYAIEIDQRSVQSAILSLKRNNIKNV
HHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCEE
TVIQSDFKKALKNLEVDTIDAMFFDPPRTGLGYDTIKQILKYKPKKLIYGSCNPSTLAKD
EEEHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
LEDLLKTYELKSLIPMDMFPYTPLVESVSLLELRTDLETV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINEQIKINDLIEIEIKKLGINGEGIGYYHKLAVFVDNALPGEIITAKVTEIFPNRLLAD
CCCCCEEECCEEEEEEEEECCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
IEEIKVKSPDRLEVMFPEYELCGAYSMQHVTYEKSLQIKRDILINALNRYVDKKIVYSKI
HHHHCCCCCCCEEEECCCHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQTIGMNEPLGYRNKVSLPVRKLEGKNKFGLYAKGGNEFIPVNDTPVHQPRINEIIQIVE
HHHHCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHH
TLMDQHKFHAYIQKDKSGYMKSMVIRRSYTTGEVQVSFLLMKKFEGIAKFTEALVAQCPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCHH
IVSVFAFYTDKYKEQIFFTNNYENLFGKATINEKINHQTFSLYPEAFFQLNTVMADKFYN
HHHHHHHHHHHHHCEEEEECCHHHHCCCEEHHHCCCCCCEEECHHHHHHHHHHHHHHHHH
KMRELANLQPTDVVIDAYAGSAPISHYIAKDVKKVYAIEIDQRSVQSAILSLKRNNIKNV
HHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCEE
TVIQSDFKKALKNLEVDTIDAMFFDPPRTGLGYDTIKQILKYKPKKLIYGSCNPSTLAKD
EEEHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
LEDLLKTYELKSLIPMDMFPYTPLVESVSLLELRTDLETV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]