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The map label for this gene is ygcA [C]

Identifier: 162447371

GI number: 162447371

Start: 533101

End: 534456

Strand: Direct

Name: ygcA [C]

Synonym: ACL_0509

Alternate gene names: 162447371

Gene position: 533101-534456 (Clockwise)

Preceding gene: 162447370

Following gene: 162447372

Centisome position: 35.61

GC content: 31.19

Gene sequence:

>1356_bases
ATGCAACAAAAAATTAGATTAAATGATATTTTAGAAATTGATGTAAAACGCATGGGTATTAATGGTGAGGGCATAGGTTA
CTATGAACGACTTGCTATTTTTATTGATAATGCACTACCTGGAGAAAAAGTCTTAGCTCAAATTACAGAAGTTTTTCCCA
ACAGAGCGACTGCTAAGGTACTACAAACATTAGAAAAGAGTCCAAATAGGGTAAAACCTTTATGTCCTGTTTATGATGCA
TGTGGTGGTTGTCAGGTACAACATTTTGATTATAATGCAATGCTTAATCAAAAAAAGGATATCATTAAAAAAGCCTTAGA
TAGATATGTCAAACGTTATGATGCACATATTATTTCAGACACAATTGGTATGGATAATCCTAAAAATTATAGAAATAAAG
CATCACTACCTTTAAGACTAATAGATAATAAAAACCGTTTTGGTATGTTTGCTAGAAACTCTAATGCTTTTGTTCCTATT
AATACATGTGGTGTTCAACATACTAAAATTAATGACATCTTTACAACTATGACAAGTCTCATGGATAGATATCACATTGA
TGCATATGACCCGGTACTACAAACTGGTATCATTTCACATGCTATTGTTAGAATTACAGAAAACTTAAATGAAGTACAAG
TTACATTTATTCTATCTAAAGCATTTGATGGTTTAGATAATCTAGTTCATGAACTAGTGAAGCTACATCCGGAAATCATG
TCTATATATAAAGTAATTAACCCAGATGTTAGACAAAGTTATTTTACTGAAGATATCCATTTAATCTATGGTAAAGAAAT
GATAGAAGAACAACTAGGTGGACATACATTTAGTCTATCACCTGATGCTTTCTTCCAACTAAACACCTCACAAGCAGATA
AATTCTATAATGAAATGAAGCGACTAGCACAAATTAAGCCAACTGAAATTGTAGTTGATGCGTATGCTGGTATTGCACCA
ATTTCACACTATATTGGTCAAGATGCTAAACATATTTATGCAATTGAGATTAATGAAGATGCGGTGAAGTCTGCAAAAAT
ATCACTGGACCAAAATCATATTGATAATGTGACTGTGATTGAAAATAATTTTGAGGATGCGCTAGATAATTTAAAAGATA
TAAAAATTGATGTCATGTTATTTGATCCTCCACGTAGTGGACTAGGAGAAGATGTTATTCAAACCATTTTAAAATATAAA
CCTAAACGATTAGTCTATGGTTCATGTAATCCATCAACACTCGCAAAGGATTTATCACTATTATTAGAGGATTATGATCT
TAAACACATTACACCAATTGATATGTTTCCATATACTAGTTTGGTGGAAAGTATCACATTACTTGAGTTAAAATAA

Upstream 100 bases:

>100_bases
TGACTCTATTTTCATAAAATAAGGCATACTTATGACCAAAATAACTCATATTTTTGGGTTTGATTTAAGTACATGTTATA
ATAATTTTGAAGGTGAAATT

Downstream 100 bases:

>100_bases
TTTGATTGAATCATCAATGAATTTAGGCTATAAAATGATTTTACTAGCTTTTTTGTTAGAATAGAAGCAATGATATGTAA
ACATTAAAACAGTATAAAAG

Product: TrmA family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQQKIRLNDILEIDVKRMGINGEGIGYYERLAIFIDNALPGEKVLAQITEVFPNRATAKVLQTLEKSPNRVKPLCPVYDA
CGGCQVQHFDYNAMLNQKKDIIKKALDRYVKRYDAHIISDTIGMDNPKNYRNKASLPLRLIDNKNRFGMFARNSNAFVPI
NTCGVQHTKINDIFTTMTSLMDRYHIDAYDPVLQTGIISHAIVRITENLNEVQVTFILSKAFDGLDNLVHELVKLHPEIM
SIYKVINPDVRQSYFTEDIHLIYGKEMIEEQLGGHTFSLSPDAFFQLNTSQADKFYNEMKRLAQIKPTEIVVDAYAGIAP
ISHYIGQDAKHIYAIEINEDAVKSAKISLDQNHIDNVTVIENNFEDALDNLKDIKIDVMLFDPPRSGLGEDVIQTILKYK
PKRLVYGSCNPSTLAKDLSLLLEDYDLKHITPIDMFPYTSLVESITLLELK

Sequences:

>Translated_451_residues
MQQKIRLNDILEIDVKRMGINGEGIGYYERLAIFIDNALPGEKVLAQITEVFPNRATAKVLQTLEKSPNRVKPLCPVYDA
CGGCQVQHFDYNAMLNQKKDIIKKALDRYVKRYDAHIISDTIGMDNPKNYRNKASLPLRLIDNKNRFGMFARNSNAFVPI
NTCGVQHTKINDIFTTMTSLMDRYHIDAYDPVLQTGIISHAIVRITENLNEVQVTFILSKAFDGLDNLVHELVKLHPEIM
SIYKVINPDVRQSYFTEDIHLIYGKEMIEEQLGGHTFSLSPDAFFQLNTSQADKFYNEMKRLAQIKPTEIVVDAYAGIAP
ISHYIGQDAKHIYAIEINEDAVKSAKISLDQNHIDNVTVIENNFEDALDNLKDIKIDVMLFDPPRSGLGEDVIQTILKYK
PKRLVYGSCNPSTLAKDLSLLLEDYDLKHITPIDMFPYTSLVESITLLELK
>Mature_451_residues
MQQKIRLNDILEIDVKRMGINGEGIGYYERLAIFIDNALPGEKVLAQITEVFPNRATAKVLQTLEKSPNRVKPLCPVYDA
CGGCQVQHFDYNAMLNQKKDIIKKALDRYVKRYDAHIISDTIGMDNPKNYRNKASLPLRLIDNKNRFGMFARNSNAFVPI
NTCGVQHTKINDIFTTMTSLMDRYHIDAYDPVLQTGIISHAIVRITENLNEVQVTFILSKAFDGLDNLVHELVKLHPEIM
SIYKVINPDVRQSYFTEDIHLIYGKEMIEEQLGGHTFSLSPDAFFQLNTSQADKFYNEMKRLAQIKPTEIVVDAYAGIAP
ISHYIGQDAKHIYAIEINEDAVKSAKISLDQNHIDNVTVIENNFEDALDNLKDIKIDVMLFDPPRSGLGEDVIQTILKYK
PKRLVYGSCNPSTLAKDLSLLLEDYDLKHITPIDMFPYTSLVESITLLELK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=292, Percent_Identity=26.3698630136986, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI51173878, Length=292, Percent_Identity=26.3698630136986, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI269784678, Length=229, Percent_Identity=29.2576419213974, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI269784680, Length=229, Percent_Identity=29.2576419213974, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI269784676, Length=229, Percent_Identity=29.2576419213974, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI20149688, Length=229, Percent_Identity=29.2576419213974, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789148, Length=456, Percent_Identity=26.7543859649123, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787083, Length=385, Percent_Identity=22.3376623376623, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790403, Length=155, Percent_Identity=30.3225806451613, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24666579, Length=473, Percent_Identity=23.0443974630021, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51316; Mature: 51316

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKIRLNDILEIDVKRMGINGEGIGYYERLAIFIDNALPGEKVLAQITEVFPNRATAKV
CCCCCCCCHHHEEEHHHCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHCCCHHHHHH
LQTLEKSPNRVKPLCPVYDACGGCQVQHFDYNAMLNQKKDIIKKALDRYVKRYDAHIISD
HHHHHCCCCCCCCCCCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIGMDNPKNYRNKASLPLRLIDNKNRFGMFARNSNAFVPINTCGVQHTKINDIFTTMTSL
HCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
MDRYHIDAYDPVLQTGIISHAIVRITENLNEVQVTFILSKAFDGLDNLVHELVKLHPEIM
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCHHHH
SIYKVINPDVRQSYFTEDIHLIYGKEMIEEQLGGHTFSLSPDAFFQLNTSQADKFYNEMK
HHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHH
RLAQIKPTEIVVDAYAGIAPISHYIGQDAKHIYAIEINEDAVKSAKISLDQNHIDNVTVI
HHHHCCCHHEEEEHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHEEEECCCCCCCEEEE
ENNFEDALDNLKDIKIDVMLFDPPRSGLGEDVIQTILKYKPKRLVYGSCNPSTLAKDLSL
ECCHHHHHCCHHHEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LLEDYDLKHITPIDMFPYTSLVESITLLELK
HHHHCCCCCCCCCCCCCHHHHHHHHHHEECC
>Mature Secondary Structure
MQQKIRLNDILEIDVKRMGINGEGIGYYERLAIFIDNALPGEKVLAQITEVFPNRATAKV
CCCCCCCCHHHEEEHHHCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHCCCHHHHHH
LQTLEKSPNRVKPLCPVYDACGGCQVQHFDYNAMLNQKKDIIKKALDRYVKRYDAHIISD
HHHHHCCCCCCCCCCCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIGMDNPKNYRNKASLPLRLIDNKNRFGMFARNSNAFVPINTCGVQHTKINDIFTTMTSL
HCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
MDRYHIDAYDPVLQTGIISHAIVRITENLNEVQVTFILSKAFDGLDNLVHELVKLHPEIM
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCHHHH
SIYKVINPDVRQSYFTEDIHLIYGKEMIEEQLGGHTFSLSPDAFFQLNTSQADKFYNEMK
HHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHH
RLAQIKPTEIVVDAYAGIAPISHYIGQDAKHIYAIEINEDAVKSAKISLDQNHIDNVTVI
HHHHCCCHHEEEEHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHEEEECCCCCCCEEEE
ENNFEDALDNLKDIKIDVMLFDPPRSGLGEDVIQTILKYKPKRLVYGSCNPSTLAKDLSL
ECCHHHHHCCHHHEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LLEDYDLKHITPIDMFPYTSLVESITLLELK
HHHHCCCCCCCCCCCCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]