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The map label for this gene is braF [H]

Identifier: 162447361

GI number: 162447361

Start: 522415

End: 523392

Strand: Direct

Name: braF [H]

Synonym: ACL_0499

Alternate gene names: 162447361

Gene position: 522415-523392 (Clockwise)

Preceding gene: 162447360

Following gene: 162447362

Centisome position: 34.9

GC content: 31.19

Gene sequence:

>978_bases
ATGTCAAAAAAGATTAATCAGATAAAACTCAATTGGCTTATTCAAAGAAAAAAAGCATTAAATCTAAAAATTGATTATTA
CAAGTTGCAAGATAAAGTGGATAGAGTAACTAGTCTGAAACTTAAACTTGATCACATCAATAAGTCTATTGAAAAGATAG
TACAACTTCAGGATAATCAAAATAGTTATTCAATCGATGAAGGTGTGATACTGGATATTCAAAATCTAACCATGAAGTTT
GGTGGATTAACAGCAGTAGATGATTTAAGTTTTAAAGTGAAAAAAGGCGAGATATTTGGACTTATCGGACCAAACGGTGC
TGGTAAAACTACAATATTTAACTGTATTACACAGTTTTATAAGACACCTCATGGACGTACTTTCTTTGTTAATAATGAAA
ATGAGTTAGTTAATTTAAGTGAGATTGCTGTGCATAACGTCATTAAAGAAGGTGTTGTAAGAACGTTCCAAAATATTGAA
CTTATTTGGGAACTATCTATCTTAGAAAACTTACTGATAGCTGGACATACATTATATACTTCAAATTTCTTTGATCACTT
ATTTAGAACCCCTAAGTATCGTAGAGAAGATAAAGTAATTCGTGCTAAAGCAATGCATATTCTTAAAGAGCTAAACTTAG
AAGCTTATACACACGTATATCCTCTAGGGTTACCTTATGGTATCTTAAAATTAGTAGAACTTGCTCGTACATTAATGACA
AATCCATCACTTATCATCTTGGATGAACCAGCTGCTGGTTTAAATGAACAAGAAACTGAAAAATTAGCACAAACCATCAA
AAAGATTCAACAAGATTTTAAAGCAACCATATTCTTGGTTGAACACGATATGGGTTTTGTGATGAATTTATGTAATACAA
TTTGTGCAATATCCTTTGGTAAAATGCTTGCCATAGGTACACCTGAAGAAATTAAGAAGCATCCTAAAGTACAAGAAGCA
TATTTGGGTGGTGAATAA

Upstream 100 bases:

>100_bases
TACCCACAAGGTTTAATTAAATTACTTGAAAAACAGTGGTTTAAACTTAAGAACTATATAAAAAACAAGATGAATAAGAA
GGGAGTTTTAGAACATGAAA

Downstream 100 bases:

>100_bases
CATGAACATTATTGAAATTAAAGATCTTGTGATCGATTACGGTATCGTAAGAGCTGTAAAAGGTGTCAACCTCAACGTAA
AAAAAGGAACCATTGTTGCC

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSKKINQIKLNWLIQRKKALNLKIDYYKLQDKVDRVTSLKLKLDHINKSIEKIVQLQDNQNSYSIDEGVILDIQNLTMKF
GGLTAVDDLSFKVKKGEIFGLIGPNGAGKTTIFNCITQFYKTPHGRTFFVNNENELVNLSEIAVHNVIKEGVVRTFQNIE
LIWELSILENLLIAGHTLYTSNFFDHLFRTPKYRREDKVIRAKAMHILKELNLEAYTHVYPLGLPYGILKLVELARTLMT
NPSLIILDEPAAGLNEQETEKLAQTIKKIQQDFKATIFLVEHDMGFVMNLCNTICAISFGKMLAIGTPEEIKKHPKVQEA
YLGGE

Sequences:

>Translated_325_residues
MSKKINQIKLNWLIQRKKALNLKIDYYKLQDKVDRVTSLKLKLDHINKSIEKIVQLQDNQNSYSIDEGVILDIQNLTMKF
GGLTAVDDLSFKVKKGEIFGLIGPNGAGKTTIFNCITQFYKTPHGRTFFVNNENELVNLSEIAVHNVIKEGVVRTFQNIE
LIWELSILENLLIAGHTLYTSNFFDHLFRTPKYRREDKVIRAKAMHILKELNLEAYTHVYPLGLPYGILKLVELARTLMT
NPSLIILDEPAAGLNEQETEKLAQTIKKIQQDFKATIFLVEHDMGFVMNLCNTICAISFGKMLAIGTPEEIKKHPKVQEA
YLGGE
>Mature_324_residues
SKKINQIKLNWLIQRKKALNLKIDYYKLQDKVDRVTSLKLKLDHINKSIEKIVQLQDNQNSYSIDEGVILDIQNLTMKFG
GLTAVDDLSFKVKKGEIFGLIGPNGAGKTTIFNCITQFYKTPHGRTFFVNNENELVNLSEIAVHNVIKEGVVRTFQNIEL
IWELSILENLLIAGHTLYTSNFFDHLFRTPKYRREDKVIRAKAMHILKELNLEAYTHVYPLGLPYGILKLVELARTLMTN
PSLIILDEPAAGLNEQETEKLAQTIKKIQQDFKATIFLVEHDMGFVMNLCNTICAISFGKMLAIGTPEEIKKHPKVQEAY
LGGE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=249, Percent_Identity=27.3092369477912, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI30795238, Length=230, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI27881501, Length=231, Percent_Identity=25.974025974026, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI45446740, Length=248, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI47078218, Length=248, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=40.1574803149606, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789991, Length=239, Percent_Identity=30.5439330543933, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789032, Length=235, Percent_Identity=27.2340425531915, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994943, Length=258, Percent_Identity=26.3565891472868, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789593, Length=253, Percent_Identity=28.8537549407115, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87081791, Length=250, Percent_Identity=26.8, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081709, Length=245, Percent_Identity=23.6734693877551, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786398, Length=235, Percent_Identity=27.6595744680851, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789672, Length=250, Percent_Identity=24, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788225, Length=249, Percent_Identity=26.9076305220884, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081782, Length=262, Percent_Identity=25.9541984732824, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788761, Length=249, Percent_Identity=23.2931726907631, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788472, Length=235, Percent_Identity=24.2553191489362, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787712, Length=251, Percent_Identity=25.0996015936255, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=24.8945147679325, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082268, Length=261, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787029, Length=252, Percent_Identity=23.8095238095238, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI48994997, Length=253, Percent_Identity=24.5059288537549, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI145693107, Length=272, Percent_Identity=25, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790190, Length=236, Percent_Identity=24.1525423728814, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790467, Length=245, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790544, Length=251, Percent_Identity=24.7011952191235, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788897, Length=224, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787500, Length=233, Percent_Identity=24.0343347639485, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790525, Length=235, Percent_Identity=25.1063829787234, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786872, Length=251, Percent_Identity=24.3027888446215, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI48995001, Length=240, Percent_Identity=25.4166666666667, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788210, Length=253, Percent_Identity=22.5296442687747, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788506, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994883, Length=261, Percent_Identity=23.3716475095785, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789963, Length=265, Percent_Identity=23.7735849056604, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787089, Length=227, Percent_Identity=22.9074889867841, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788450, Length=247, Percent_Identity=23.4817813765182, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787105, Length=195, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789962, Length=242, Percent_Identity=21.900826446281, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786803, Length=241, Percent_Identity=22.4066390041494, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789586, Length=248, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081834, Length=225, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787792, Length=240, Percent_Identity=24.1666666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786703, Length=214, Percent_Identity=25.2336448598131, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788644, Length=258, Percent_Identity=23.2558139534884, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=245, Percent_Identity=29.3877551020408, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115534520, Length=273, Percent_Identity=20.5128205128205, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533971, Length=230, Percent_Identity=24.7826086956522, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=239, Percent_Identity=23.0125523012552, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI28574150, Length=318, Percent_Identity=24.5283018867925, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007328, Length=318, Percent_Identity=24.5283018867925, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24641342, Length=244, Percent_Identity=27.4590163934426, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221512771, Length=270, Percent_Identity=27.037037037037, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 37092; Mature: 36961

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKINQIKLNWLIQRKKALNLKIDYYKLQDKVDRVTSLKLKLDHINKSIEKIVQLQDNQ
CCCCCHHEEHHHHEEHHHHCEEEEEEEHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCC
NSYSIDEGVILDIQNLTMKFGGLTAVDDLSFKVKKGEIFGLIGPNGAGKTTIFNCITQFY
CCEECCCCEEEEHHHHHHHHCCEEEEECCEEEEECCCEEEEECCCCCCHHHHHHHHHHHH
KTPHGRTFFVNNENELVNLSEIAVHNVIKEGVVRTFQNIELIWELSILENLLIAGHTLYT
HCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCHHHH
SNFFDHLFRTPKYRREDKVIRAKAMHILKELNLEAYTHVYPLGLPYGILKLVELARTLMT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHHHC
NPSLIILDEPAAGLNEQETEKLAQTIKKIQQDFKATIFLVEHDMGFVMNLCNTICAISFG
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHC
KMLAIGTPEEIKKHPKVQEAYLGGE
CEEECCCHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure 
SKKINQIKLNWLIQRKKALNLKIDYYKLQDKVDRVTSLKLKLDHINKSIEKIVQLQDNQ
CCCCHHEEHHHHEEHHHHCEEEEEEEHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCC
NSYSIDEGVILDIQNLTMKFGGLTAVDDLSFKVKKGEIFGLIGPNGAGKTTIFNCITQFY
CCEECCCCEEEEHHHHHHHHCCEEEEECCEEEEECCCEEEEECCCCCCHHHHHHHHHHHH
KTPHGRTFFVNNENELVNLSEIAVHNVIKEGVVRTFQNIELIWELSILENLLIAGHTLYT
HCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCHHHH
SNFFDHLFRTPKYRREDKVIRAKAMHILKELNLEAYTHVYPLGLPYGILKLVELARTLMT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHHHC
NPSLIILDEPAAGLNEQETEKLAQTIKKIQQDFKATIFLVEHDMGFVMNLCNTICAISFG
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHC
KMLAIGTPEEIKKHPKVQEAYLGGE
CEEECCCHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]