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The map label for this gene is nfo

Identifier: 162447345

GI number: 162447345

Start: 506191

End: 507075

Strand: Direct

Name: nfo

Synonym: ACL_0482

Alternate gene names: 162447345

Gene position: 506191-507075 (Clockwise)

Preceding gene: 162447344

Following gene: 162447346

Centisome position: 33.81

GC content: 34.35

Gene sequence:

>885_bases
ATGCTGATCTTAGGAAGTCACGTAAGTCTCAAGGGGAATGATATGTTCTATGGTAGTGTATTAGAGGCACTCGAATACAA
TGCAAATACCATGATGGTATATACCGGAGCCCCACAAAATACAATTAGAAAACCAATTGAAACAATGAAAATAGAAGAGG
CGCATGCACTCATGAAGGAAAAAGGTATTGATTTAAATCATGTCGTGGTGCATGCACCTTACATTATTAATTTATGTAAC
CCAGATCCAGAACGTCGTGCATTTGCTGTTGAGTTTCTTACAAAAGAAGTCATTCGTGTTGGACAAATGGGCATTAACCA
AATGGTTTTACACCCGGGTTCAGCAGTTGGTGGTAATAGAGAAGAAGCGGTTCAATGGATTAGTGAAGGCTTAAATCAAA
TTATCGATAACACTAAATCATACAACGTCAGAATTGCTTTAGAAACGATGGCTGGAAAAGGCAATGAAATTGGTAAAACC
TTTGAAGAAATCAAAGCAATCATAGATGGCGTTGATGATAAATCAAGAGTATCTGTTTGTTTTGATACATGTCACGTACA
TGATGCAGGTTATGATGTCATTCATGATCTAGATAAAACACTCCAATTATTTGATGATATTGTAGGATTAAACTATATTT
CTGTAGTCCACGTAAACGACTCTAAAAATGAATTAGGTGCTTCCAAAGACCGTCATGAAAATATCGGTTTTGGCTATATA
GGTTATGACGCTTTACTAAAAGTGATTTATCATGAAGCATTTAAAGAGATTCCTAAAATACTAGAAACTCCGTATGTAAA
TGATAAACCACCATACAAATTAGAAATTCAAATGATTAAAGATAGAGTATTTGATAACGAATTAAAAACAAAGCTAGAAA
AATAA

Upstream 100 bases:

>100_bases
AAAATAGCCAAACCAAAGCGTGTCACACCAGGCTATAAGAAAAAACACGCGGAAAAAGTTAAAGCTGCAATTAAAAAGGT
AAAGAAAGGTAGGTACAGGG

Downstream 100 bases:

>100_bases
CATTGAATTAACATTTTAATCATGTTATAATTATATTTGCGAAAACTATAAGGAGAATTTGACATGGCAAAGAAATATCG
TGCTGTTGAAAGCAAAATTC

Product: endodeoxyribonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKEKGIDLNHVVVHAPYIINLCN
PDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNREEAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKT
FEEIKAIIDGVDDKSRVSVCFDTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYI
GYDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK

Sequences:

>Translated_294_residues
MLILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKEKGIDLNHVVVHAPYIINLCN
PDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNREEAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKT
FEEIKAIIDGVDDKSRVSVCFDTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYI
GYDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK
>Mature_294_residues
MLILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKEKGIDLNHVVVHAPYIINLCN
PDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNREEAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKT
FEEIKAIIDGVDDKSRVSVCFDTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYI
GYDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=266, Percent_Identity=34.5864661654135, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17531193, Length=270, Percent_Identity=36.2962962962963, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322735, Length=284, Percent_Identity=35.5633802816901, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_ACHLI (A9NFH1)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620477.1
- ProteinModelPortal:   A9NFH1
- SMR:   A9NFH1
- GeneID:   5804398
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0482
- HOGENOM:   HBG565018
- OMA:   QIALETM
- BioCyc:   ALAI441768:ACL_0482-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 33121; Mature: 33121

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKE
CEEECCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KGIDLNHVVVHAPYIINLCNPDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNR
CCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCH
EEAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKTFEEIKAIIDGVDDKSRVSVC
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHEEE
FDTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYI
EHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCCCCCCCEEE
GYDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKE
CEEECCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KGIDLNHVVVHAPYIINLCNPDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNR
CCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCH
EEAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKTFEEIKAIIDGVDDKSRVSVC
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHEEE
FDTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYI
EHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCCCCCCCEEE
GYDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA