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The map label for this gene is ptsA [H]

Identifier: 162447301

GI number: 162447301

Start: 458065

End: 459777

Strand: Direct

Name: ptsA [H]

Synonym: ACL_0436

Alternate gene names: 162447301

Gene position: 458065-459777 (Clockwise)

Preceding gene: 162447300

Following gene: 162447302

Centisome position: 30.6

GC content: 32.75

Gene sequence:

>1713_bases
ATGACAAAACTTAAAGGTAATGGTGTATCCAGTGGTATAGCAATTGCTAAGGTACATCAATTCAAACTAAATAACATGTT
TGCAATGAATAAGTATGCAACCGACAAAGCATTTGAAATCAAGCGCTTAGGGGATGCACTTTATAAAGCAGATAAGGAAC
TTGAAAATCTAACCAAGCAAGCTGAAGAAAGAATTGGGGTTGAACATGCCCAAATCTTTAGTGCGCAACAAATGATGATT
AATGACCCTGAAATGATAAAAAATGCGCATGTTAAAATAAATGACTTTAATCTAGATGCTGCCTACGCATTTAAGCAAAC
TATGAGTGAAATGATAGATATGTTTGAGTTAAGTGATAATCCATACATTAAGGAACGTATCTCTGATTTAGAAGATATTT
CAAATAGAGTATTACATATTTTAAGTGGAAAAACGCATAGTCTTTATGTGTTTAATGAAGATGTGATACTTGTAGCAACA
GACTTAGTCCCTTCAGAAACAATCAATTTAGACACAACTTTTATTAAAGGTATTGTCATTGAAAAAGGATCTAAAACAAG
TCACTCAGCAATTCTTGCACGTAATTTAGGTATTCCTGCAATTACGGGTGTTGATGTAAGTAAAATACATCACGACACAC
TTGCAATCATTGATGGACACACAGGGGATATTTTATTAGAACCTAGTGAAGATACCATCAAAACATACCAAACTAAACAG
CAAGAAGCACTTAAATTACAATCAACGTATGAAACAATAAAAGACCTTAAAACAGCAACCATAGACAATCATGAAGTCCA
TTTAGCTGCAAACATTGGTGTTGATTTAGACATTCAAGAAGCACTAGATGCTAATGCTTCAGGTATTGGTTTATTACGTA
CAGAAAACCAGTATTTAGAATCTGATGATTTTCCAACCGAAGATGAACTATTTATCTTCTATGAAAAGGTAGCAACATCA
TTTGAAACTAATGAAGTCACTATTAGAACGCTTGATATTGGTGGCGATAAAAACTTAAGTTACCTCAATATGCGTAAAGA
ATTAAACCCATTTTTAGGTAATAGAGCAATCAGATATAGCCTAAGTTATCCATCATTATTTAAAACTCAGCTTCGTGCTA
TCTTACGTGCTAATACACATCAAAATATTAAAGTCATGTTTCCAATGATTTCAACTTTAAAAGAACTCATGGATGCGAAG
AAGATACTAGATGAAACTTATCAAGCATTAAAGATTGAAGGACATGAAGTAAATTATCCAAAGATTGGTATGATGGTTGA
AGTACCATCCGCTGCACTTGGTATAGAAAAGTTTTTACCACATATTGATTTCATCAGTATTGGAACCAATGACCTGATTC
AATACATGTTTGCAGCAGATAGAATGAATGAACGTGTAGCATATCTTTATCAACCATATAATCCAATTCTTTTGTCCACC
ATCAAATCTATGATTGACAAGACTAATAAGCATGGTATAATAGTTAGCGTGTGCGGTGAAATGGCTGGCCATAAAGCTCA
AAGTTTACTACTTATTGGCTTAGGTATCAAACATTTAAGCATGCATGCAAACTTAATTTTAGAAAACAAATACTTGATTT
CTAAAACAACTTATAAGGCACTAGAAGAAATTGCCGAAAAAGCATTATTACTAGATCACCCTTACGAAGTTGAAGCACTT
ATAAATACATATATTAAAGGACTTAAATATTAA

Upstream 100 bases:

>100_bases
TAAGTAACATTGTAAACTATGTGACAACATCACTAGCGATAGTTAAAGATAACGATTTTGCAATTGTAGAGGCATTCAAA
AGAGAGGTTATTTAACCCTA

Downstream 100 bases:

>100_bases
GTCCTTCACACGGAACAATACTCAAGAGGCTGAAGAGGCACGCTTGGAAAGCGTGTAGGGTGTAATAGCCGCGAGGGTTC
AAATCCCTCTTGTTCCGCCA

Product: phosphoenolpyruvate-protein kinase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQAEERIGVEHAQIFSAQQMMI
NDPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDNPYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVAT
DLVPSETINLDTTFIKGIVIEKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQ
QEALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLESDDFPTEDELFIFYEKVATS
FETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYSLSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAK
KILDETYQALKIEGHEVNYPKIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLST
IKSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKALEEIAEKALLLDHPYEVEAL
INTYIKGLKY

Sequences:

>Translated_570_residues
MTKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQAEERIGVEHAQIFSAQQMMI
NDPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDNPYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVAT
DLVPSETINLDTTFIKGIVIEKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQ
QEALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLESDDFPTEDELFIFYEKVATS
FETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYSLSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAK
KILDETYQALKIEGHEVNYPKIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLST
IKSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKALEEIAEKALLLDHPYEVEAL
INTYIKGLKY
>Mature_569_residues
TKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQAEERIGVEHAQIFSAQQMMIN
DPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDNPYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVATD
LVPSETINLDTTFIKGIVIEKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQQ
EALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLESDDFPTEDELFIFYEKVATSF
ETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYSLSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAKK
ILDETYQALKIEGHEVNYPKIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLSTI
KSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKALEEIAEKALLLDHPYEVEALI
NTYIKGLKY

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:
GO:0003824: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0005351: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0005737: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0008965: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0009401: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0016301: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0016310: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0016740: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0016772: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
GO:0046872: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=563, Percent_Identity=36.4120781527531, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI1789193, Length=555, Percent_Identity=33.5135135135135, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=33.9191564147627, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI48994992, Length=475, Percent_Identity=34.3157894736842, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI1787994, Length=404, Percent_Identity=28.4653465346535, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI226510935, Length=150, Percent_Identity=34, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64186; Mature: 64055

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQ
CCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEERIGVEHAQIFSAQQMMINDPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDN
HHHHHCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
PYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVATDLVPSETINLDTTFIKGIVI
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHEEEEEEE
EKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQ
ECCCCCCCHHHHHHCCCCCEECCCCHHHCCCCEEEEEECCCCCEEECCCHHHHHHHHHHH
QEALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLE
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHCCCCCCEEEEECCCCCCC
SDDFPTEDELFIFYEKVATSFETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYS
CCCCCCCCCEEEEEEHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCHHHCCCEEEEE
LSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAKKILDETYQALKIEGHEVNYP
ECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
KIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLST
CEEEEEECCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
IKSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKA
HHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECHHHHHEEEEEEEECCEEEHHHHHHH
LEEIAEKALLLDHPYEVEALINTYIKGLKY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQ
CCCCCCCCCCCEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEERIGVEHAQIFSAQQMMINDPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDN
HHHHHCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
PYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVATDLVPSETINLDTTFIKGIVI
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHEEEEEEE
EKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQ
ECCCCCCCHHHHHHCCCCCEECCCCHHHCCCCEEEEEECCCCCEEECCCHHHHHHHHHHH
QEALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLE
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHCCCCCCEEEEECCCCCCC
SDDFPTEDELFIFYEKVATSFETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYS
CCCCCCCCCEEEEEEHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCHHHCCCEEEEE
LSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAKKILDETYQALKIEGHEVNYP
ECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
KIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLST
CEEEEEECCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
IKSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKA
HHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECHHHHHEEEEEEEECCEEEHHHHHHH
LEEIAEKALLLDHPYEVEALINTYIKGLKY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]