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The map label for this gene is eno

Identifier: 162447267

GI number: 162447267

Start: 422407

End: 423702

Strand: Direct

Name: eno

Synonym: ACL_0402

Alternate gene names: 162447267

Gene position: 422407-423702 (Clockwise)

Preceding gene: 162447266

Following gene: 162447268

Centisome position: 28.22

GC content: 35.96

Gene sequence:

>1296_bases
ATGCCATTTATTACAAGCGTATATGCTAGAGAAGTACTTGATAGCCGCGGTAACCCAACTGTTGAAGTTGAAATCGTTAC
AGACTCAGGTGCATTTGGTAGAACATTAGTACCATCAGGTGCTTCAACAGGTGAACACGAAGCTGTTGAATTACGTGACG
GTGATAAATCACGTTACTTAGGTAAAGGTGTTTTAAAAGCTGTTGAAAATGTTAATGACATTATCGGACCAGCAATCTTA
GGAGATTCAGTTTTAGATCAAGTAGCTCTTGATAGAAAATTAATCGCTTTAGATGGTACAAAAAACAAAGGTAAATTAGG
TGCAAACGCTATTTTAGGTGTTTCAATCGCTGCTGCTAAAGCTGCTGCTGATTTATTAAACCTTGAATTATACCAATACT
TAGGCGGATTTAACGCTAAACAATTACCAGTACCAATGATGAATATCATCAATGGTGGTGCACACTCAGATGCTCCAATC
GACTTCCAAGAATTCATGATTTTCCCAGTAGGTGCGCCTTCATTTAAAGAAGCTATCCGTTGGGGAGCTGAAATCTTCCA
TGCTTTAAAAGCTATTTTAAAGAAAAAAGGTCTTTCAACTGCAGTAGGTGATGAAGGTGGATTTGCTCCAAACTTAGCAT
CTAATGAAGATACAATTGATAACATCTTAGAAGCTATTAAAAATGCTGGTTATAAAGCTGGTGAAGAAGTATTTATCGGT
TTTGACGTAGCTGCTTCAGAATTCTTTGATAAAGCTAAAGGTAAATATGTATTCAAAAAATCTACTAAAGAAGAATTTAC
TTCTGCTGAATTAGTAGAATACTATGCTGGATTAGTTCAAAAATACCCAATCATCTCAATCGAAGATGGTATGGATGAAA
ATGACTGGGATGGCTGGAAGTTATTAACTGACAAATTAGGTTCAAAAATCCAATTAGTTGGGGATGACCTATTTGTTACA
AACACTGAATATCTTGCAAGAGGTATCAAAACAAACACAGCAAACTCAATTTTAGTTAAAGTAAACCAAATCGGTACATT
AACTGAAACATTTGACGCAATCGAAATGGCTAAACGTGCTGGTTATACAGCAGTTATCTCACACCGTTCAGGTGAAACAG
AAGATACAACAATTGCTGATATCGCTGTTGCTGCAAATACAGGACAAATTAAAACTGGTTCAGCTTCACGTACAGACCGT
GTTGCTAAATACAACCAATTAATGCGTATTGAAGATCAACTAGGTGAAGATGCTGTTTACTTAGGTAAAGATTCTTTCTA
CAACCTAAAAAAATAA

Upstream 100 bases:

>100_bases
TATGCGTTTTCATTGCAAGATTACTTTCACTAAAGTTTTAATTATTGTATAATATAGGTGGAAAAATTCTATAAAAAATT
TTATAAAGGAGAATTAAAAT

Downstream 100 bases:

>100_bases
GTTTAAAACCTTAAAAGAAAGTGCTGCAATTTATTTGCAGCACTTTTTATTATGTTTTATAAGATAAATGATATATATTT
CATATATAAAATCCATATTC

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPFITSVYAREVLDSRGNPTVEVEIVTDSGAFGRTLVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVENVNDIIGPAIL
GDSVLDQVALDRKLIALDGTKNKGKLGANAILGVSIAAAKAAADLLNLELYQYLGGFNAKQLPVPMMNIINGGAHSDAPI
DFQEFMIFPVGAPSFKEAIRWGAEIFHALKAILKKKGLSTAVGDEGGFAPNLASNEDTIDNILEAIKNAGYKAGEEVFIG
FDVAASEFFDKAKGKYVFKKSTKEEFTSAELVEYYAGLVQKYPIISIEDGMDENDWDGWKLLTDKLGSKIQLVGDDLFVT
NTEYLARGIKTNTANSILVKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDTTIADIAVAANTGQIKTGSASRTDR
VAKYNQLMRIEDQLGEDAVYLGKDSFYNLKK

Sequences:

>Translated_431_residues
MPFITSVYAREVLDSRGNPTVEVEIVTDSGAFGRTLVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVENVNDIIGPAIL
GDSVLDQVALDRKLIALDGTKNKGKLGANAILGVSIAAAKAAADLLNLELYQYLGGFNAKQLPVPMMNIINGGAHSDAPI
DFQEFMIFPVGAPSFKEAIRWGAEIFHALKAILKKKGLSTAVGDEGGFAPNLASNEDTIDNILEAIKNAGYKAGEEVFIG
FDVAASEFFDKAKGKYVFKKSTKEEFTSAELVEYYAGLVQKYPIISIEDGMDENDWDGWKLLTDKLGSKIQLVGDDLFVT
NTEYLARGIKTNTANSILVKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDTTIADIAVAANTGQIKTGSASRTDR
VAKYNQLMRIEDQLGEDAVYLGKDSFYNLKK
>Mature_430_residues
PFITSVYAREVLDSRGNPTVEVEIVTDSGAFGRTLVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVENVNDIIGPAILG
DSVLDQVALDRKLIALDGTKNKGKLGANAILGVSIAAAKAAADLLNLELYQYLGGFNAKQLPVPMMNIINGGAHSDAPID
FQEFMIFPVGAPSFKEAIRWGAEIFHALKAILKKKGLSTAVGDEGGFAPNLASNEDTIDNILEAIKNAGYKAGEEVFIGF
DVAASEFFDKAKGKYVFKKSTKEEFTSAELVEYYAGLVQKYPIISIEDGMDENDWDGWKLLTDKLGSKIQLVGDDLFVTN
TEYLARGIKTNTANSILVKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGETEDTTIADIAVAANTGQIKTGSASRTDRV
AKYNQLMRIEDQLGEDAVYLGKDSFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:
GO:0000015: Enolase
GO:0000287: Enolase
GO:0004634: Enolase
GO:0005576: Enolase
GO:0005737: Enolase
GO:0006096: Enolase
GO:0009986: Enolase
GO:0016829: Enolase
GO:0046872: Enolase

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=436, Percent_Identity=51.1467889908257, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=436, Percent_Identity=51.1467889908257, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=51.6279069767442, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=49.0697674418605, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=434, Percent_Identity=45.6221198156682, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=25.8823529411765, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=25.8823529411765, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=25.8823529411765, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=58.7822014051522, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=52.3041474654378, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=52.3041474654378, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.875, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=50.5800464037123, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=50.5800464037123, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=50.3480278422274, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=50.2293577981651, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.3464203233256, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.3023255813954, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.3023255813954, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.3023255813954, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.3023255813954, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=49.3023255813954, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=49.3023255813954, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ACHLI (A9NF93)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620399.1
- ProteinModelPortal:   A9NF93
- SMR:   A9NF93
- GeneID:   5803119
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0402
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   ALAI441768:ACL_0402-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46517; Mature: 46386

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 340-340 BINDING 155-155 BINDING 164-164 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFITSVYAREVLDSRGNPTVEVEIVTDSGAFGRTLVPSGASTGEHEAVELRDGDKSRYL
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNDIIGPAILGDSVLDQVALDRKLIALDGTKNKGKLGANAILGVSIAAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEHHHHHHHH
AAADLLNLELYQYLGGFNAKQLPVPMMNIINGGAHSDAPIDFQEFMIFPVGAPSFKEAIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGAEIFHALKAILKKKGLSTAVGDEGGFAPNLASNEDTIDNILEAIKNAGYKAGEEVFIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
FDVAASEFFDKAKGKYVFKKSTKEEFTSAELVEYYAGLVQKYPIISIEDGMDENDWDGWK
EEHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
LLTDKLGSKIQLVGDDLFVTNTEYLARGIKTNTANSILVKVNQIGTLTETFDAIEMAKRA
HHHHHCCCEEEEECCCEEEECHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVISHRSGETEDTTIADIAVAANTGQIKTGSASRTDRVAKYNQLMRIEDQLGEDAVY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LGKDSFYNLKK
ECCCCCCCCCC
>Mature Secondary Structure 
PFITSVYAREVLDSRGNPTVEVEIVTDSGAFGRTLVPSGASTGEHEAVELRDGDKSRYL
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNDIIGPAILGDSVLDQVALDRKLIALDGTKNKGKLGANAILGVSIAAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEHHHHHHHH
AAADLLNLELYQYLGGFNAKQLPVPMMNIINGGAHSDAPIDFQEFMIFPVGAPSFKEAIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGAEIFHALKAILKKKGLSTAVGDEGGFAPNLASNEDTIDNILEAIKNAGYKAGEEVFIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
FDVAASEFFDKAKGKYVFKKSTKEEFTSAELVEYYAGLVQKYPIISIEDGMDENDWDGWK
EEHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
LLTDKLGSKIQLVGDDLFVTNTEYLARGIKTNTANSILVKVNQIGTLTETFDAIEMAKRA
HHHHHCCCEEEEECCCEEEECHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVISHRSGETEDTTIADIAVAANTGQIKTGSASRTDRVAKYNQLMRIEDQLGEDAVY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LGKDSFYNLKK
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA