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The map label for this gene is tpx [H]

Identifier: 162447212

GI number: 162447212

Start: 362361

End: 362855

Strand: Direct

Name: tpx [H]

Synonym: ACL_0346

Alternate gene names: 162447212

Gene position: 362361-362855 (Clockwise)

Preceding gene: 162447211

Following gene: 162447213

Centisome position: 24.21

GC content: 29.49

Gene sequence:

>495_bases
ATGAAAACAATGAAAAACAAACCCATAACAGTTTTAGGGGAACCATTACAGATTGGTGATAAAGCACTCGACTTTATCGC
TGTAGATAATAATTTAAATGAAGTATCATTTTTTGCTAATTTTAAAAAAGACTATACACTGATTAGCGCTGTACCTAGTT
TAGATACATCTGTATGTGATATTCAAACACGTACAATTAATGAAAGATTATCGAGTTATAAAAACTTAGATTTTATTACC
ATTTCTAATGACCTACCATTTGCACAAAAAAGATGGTGTGGCTCAAATGGTCTAGAAATCACAACTTTAAGTGACCACAA
ACTCTTGGATTTTGGTATGAAGTATGGTGCACTTATTAAAGAACTTAGATTACTTGCAAGATCTATTTTCGTTTTAGACC
ATCAAAGAAAGGTAGTTTATGTTGAATATTTAGATGAAATGAGCAATCATCCGAATTATGATAAACTATTTAAATTTTTA
GATACTGTACTATAA

Upstream 100 bases:

>100_bases
ATGTTAAGAGCTAAAAGAACCTAAAACAAGTCAGGGCGAAAATGACTAAAATATTTACCTTCTATCCTATAATAGACTTA
TAAGGATAGGAGGTATTTTT

Downstream 100 bases:

>100_bases
CAAAAAAAACAAGGGCAAGAAGATAACCATAATCTGTCAAAGAGCCTTTATAAAAATATGTAATTATGATAAAATACTAT
AGATAAATAAAGGAAGTGAC

Product: peroxiredoxin family protein

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: Superoxide-inducible protein 8; SOI8 [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKTMKNKPITVLGEPLQIGDKALDFIAVDNNLNEVSFFANFKKDYTLISAVPSLDTSVCDIQTRTINERLSSYKNLDFIT
ISNDLPFAQKRWCGSNGLEITTLSDHKLLDFGMKYGALIKELRLLARSIFVLDHQRKVVYVEYLDEMSNHPNYDKLFKFL
DTVL

Sequences:

>Translated_164_residues
MKTMKNKPITVLGEPLQIGDKALDFIAVDNNLNEVSFFANFKKDYTLISAVPSLDTSVCDIQTRTINERLSSYKNLDFIT
ISNDLPFAQKRWCGSNGLEITTLSDHKLLDFGMKYGALIKELRLLARSIFVLDHQRKVVYVEYLDEMSNHPNYDKLFKFL
DTVL
>Mature_164_residues
MKTMKNKPITVLGEPLQIGDKALDFIAVDNNLNEVSFFANFKKDYTLISAVPSLDTSVCDIQTRTINERLSSYKNLDFIT
ISNDLPFAQKRWCGSNGLEITTLSDHKLLDFGMKYGALIKELRLLARSIFVLDHQRKVVYVEYLDEMSNHPNYDKLFKFL
DTVL

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2) [H]

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:
GO:0004601: Peroxiredoxin family protein
GO:0016491: Peroxiredoxin family protein
GO:0016684: Peroxiredoxin family protein
GO:0055114: Peroxiredoxin family protein

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1787584, Length=160, Percent_Identity=32.5, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: 1.11.1.- [C]

Molecular weight: Translated: 18832; Mature: 18832

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMKNKPITVLGEPLQIGDKALDFIAVDNNLNEVSFFANFKKDYTLISAVPSLDTSVCD
CCCCCCCCEEEECCCHHHCCHHEEEEEECCCCCHHHHEECCCCCEEEEECCCCCCCHHHH
IQTRTINERLSSYKNLDFITISNDLPFAQKRWCGSNGLEITTLSDHKLLDFGMKYGALIK
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ELRLLARSIFVLDHQRKVVYVEYLDEMSNHPNYDKLFKFLDTVL
HHHHHHHHHHHEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKTMKNKPITVLGEPLQIGDKALDFIAVDNNLNEVSFFANFKKDYTLISAVPSLDTSVCD
CCCCCCCCEEEECCCHHHCCHHEEEEEECCCCCHHHHEECCCCCEEEEECCCCCCCHHHH
IQTRTINERLSSYKNLDFITISNDLPFAQKRWCGSNGLEITTLSDHKLLDFGMKYGALIK
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ELRLLARSIFVLDHQRKVVYVEYLDEMSNHPNYDKLFKFLDTVL
HHHHHHHHHHHEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]